Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,137	2 bp→CA	intergenic (‑9480/‑63080)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,137	0	A	C	100.0%	135.0 / NA	47	intergenic (‑9480/‑63081)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/27);  total (20/27)
*	1	859,138	0	G	A	100.0%	124.3 / NA	47	intergenic (‑9481/‑63080)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/27);  total (20/27)

ACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAA  >  1/858916‑859293                                                                                                                                                                                                                              ||                                                                                                                                                            aCGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAAc                                                                                                                                   >  1:341443/1‑249 (MQ=255)  cGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGTCTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCtt                                                                                                                                >  1:92207/1‑251 (MQ=255)       tACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTcgac                                                                                                                           <  1:227691/251‑4 (MQ=255)         cacCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGAccg                                                                                                                         >  1:322667/1‑250 (MQ=255)               gATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGAccgtttc                                                                                                                     >  1:808615/1‑244 (MQ=255)                 tttCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTaa                                                                                                                 <  1:430707/251‑1 (MQ=255)                    ccATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGc                                                                                                              <  1:225218/251‑1 (MQ=255)                      aTTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCtt                                                                                                            <  1:412058/251‑1 (MQ=255)                                  tCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTc                                                                                                <  1:82274/251‑1 (MQ=255)                                       aaCCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGa                                                                                           <  1:647886/251‑1 (MQ=255)                                                 aCCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGc                                                                                 <  1:276557/251‑1 (MQ=255)                                                        aCTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttcttc                                                                          <  1:481702/251‑1 (MQ=255)                                                         cTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTc                                                                                                                                         <  1:804591/187‑1 (MQ=255)                                                         cTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttctt                                                                           >  1:494294/1‑249 (MQ=255)                                                           aaTGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTg                                                                       <  1:686430/251‑1 (MQ=255)                                                            aTGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGc                                                                                                              <  1:305182/211‑1 (MQ=255)                                                                             aCGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttacctt                                                      >  1:766454/1‑245 (MQ=255)                                                                              tattccgttctttAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttaccttca                                                    >  1:175643/12‑244 (MQ=255)                                                                                   cTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTa                                                >  1:548782/1‑250 (MQ=255)                                                                                     ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                             <  1:769744/251‑1 (MQ=255)                                                                                     ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                             <  1:17735/251‑1 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTcgac                                                                                                                           <  1:175589/171‑4 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGa                                                                                           >  1:391127/1‑203 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTgtg                                                                     <  1:40864/225‑3 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:159963/1‑250 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:806362/1‑250 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:878691/1‑250 (MQ=255)                                                                                       aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:237683/1‑250 (MQ=255)                                                                                        gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                          >  1:117591/1‑249 (MQ=255)                                                                                        gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                          >  1:226130/1‑249 (MQ=255)                                                                                          tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAGCACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                        <  1:653579/251‑1 (MQ=255)                                                                                          tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                        <  1:894327/251‑1 (MQ=255)                                                                                          tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                        <  1:340674/251‑1 (MQ=255)                                                                                               cccATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                   <  1:781966/251‑4 (MQ=255)                                                                                                  atcgCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTAc                                <  1:244491/248‑1 (MQ=255)                                                                                                            aaaGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACtttcttcac                       <  1:216054/250‑7 (MQ=255)                                                                                                                tttcttatttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACagat                   <  1:176042/246‑1 (MQ=255)                                                                                                                      ttttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAg            <  1:837636/250‑1 (MQ=255)                                                                                                                         taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt         <  1:795982/251‑1 (MQ=255)                                                                                                                         taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt         <  1:862888/251‑1 (MQ=255)                                                                                                                            ctctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTg      >  1:251446/1‑251 (MQ=255)                                                                                                                              ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttcttc                                                                          <  1:361749/181‑1 (MQ=255)                                                                                                                              ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga    >  1:742506/1‑250 (MQ=255)                                                                                                                              ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga    >  1:735964/1‑250 (MQ=255)                                                                                                                              ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga    >  1:591569/1‑250 (MQ=255)                                                                                                                                acaacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTggaaa  <  1:567193/251‑4 (MQ=255)                                                                                                                                 caacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                   >  1:546111/1‑214 (MQ=255)                                                                                                                                                                                                                              ||                                                                                                                                                            ACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAA  >  1/858916‑859293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑