Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	857,107	2 bp→AG	intergenic (‑7450/‑65110)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	857,107	0	G	A	100.0%	68.0 / NA	27	intergenic (‑7450/‑65111)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/10);  total (17/10)
*	1	857,108	0	A	G	100.0%	73.6 / NA	27	intergenic (‑7451/‑65110)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/10);  total (17/10)

GAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAA‑TATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTT  >  1/856875‑857327                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                             gAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAGCTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAa‑tatat                                                                                                                                                                                                             >  1:860437/1‑248 (MQ=255)                       ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCTGTTAAA‑TATATCTAAATTAGGAGCATATTCAATa                                                                                                                                                                                      <  1:206159/251‑1 (MQ=255)                       ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAAtg                                                                                                                                                                                      <  1:206128/251‑2 (MQ=255)                                      tactgcgaatacgcTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGc                                                                                                                                                                       <  1:504066/240‑1 (MQ=255)                                                   ctcacATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGCTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAaata                                                                                                                                                          >  1:26506/5‑251 (MQ=255)                                                             tCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAAtaaatctaa                                                                                                                                                >  1:32018/1‑251 (MQ=255)                                                                                            cgatttttctttctACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAa‑ta                                                                                                                                                                                                                <  1:424188/150‑1 (MQ=255)                                                                                               tttttctttctACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGc                                                                                                              <  1:496521/248‑1 (MQ=255)                                                                                                                         ttattatAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGAt                                                                                                                                                                          >  1:573624/2‑165 (MQ=255)                                                                                                                                                             tttttgacgttATAGATAGGTTGATCGCAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAACTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTgcgc                                                 >  1:158595/10‑250 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATTATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATc                                                                                                                                                               >  1:467729/2‑133 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAAtt                                                                                                                                                                                                      >  1:261018/2‑93 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAgc                                                                                                                                                                                                <  1:306341/98‑2 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGc                                                                                                                                                                       >  1:634047/2‑124 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                         >  1:817751/2‑249 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                         >  1:477338/2‑249 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                         >  1:711880/2‑249 (MQ=255)                                                                                                                                                                     gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                         >  1:556451/2‑249 (MQ=255)                                                                                                                                                                        atagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgcc                                       >  1:7724/1‑246 (MQ=255)                                                                                                                                                                         tagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgccctt                                    >  1:367582/1‑245 (MQ=255)                                                                                                                                                                                gTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAAc                               <  1:252111/249‑1 (MQ=255)                                                                                                                                                                                    aTCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTa                                                                                                                                <  1:470018/148‑1 (MQ=255)                                                                                                                                                                                    aTCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                   <  1:637905/193‑1 (MQ=255)                                                                                                                                                                                                      gTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTAt       >  1:407631/1‑251 (MQ=255)                                                                                                                                                                                                           tttCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGACAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCtt  >  1:538659/1‑251 (MQ=255)                                                                                                                                                                                                                               aGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCaa                   <  1:347677/214‑1 (MQ=255)                                                                                                                                                                                                                                gCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                        >  1:748852/1‑208 (MQ=255)                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                             GAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAA‑TATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTT  >  1/856875‑857327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑