| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 855,519 | 2 bp→TT | intergenic (‑5862/‑66698) | unknown ← / → unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 855,519 | 0 | C | T | 90.0% | 16.2 / ‑4.1 | 10 | intergenic (‑5862/‑66699) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (2/7); total (2/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | 1 | 855,520 | 0 | C | T | 100.0% | 22.2 / NA | 10 | intergenic (‑5863/‑66698) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (2/8); total (2/8) | |||||||||||
GTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACTTTCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCA > 1/855401‑855754 || gTTAGTAGTCCGTGATTAATAAACTCGCCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCTTGCAAAACGATTTGACCGTTTTCCATTCCCATAACTTCAGTGATATTCATAATTTTTCTTGAACCATCGTTTAAGCGGGACGCCTGCACTATTATGTTTACTGCTGAAGAAATATTACGGCGAATTGCCTCCAACGGTAGGGATGCATTCGACATCATAAcc < 1:507049/246‑1 (MQ=255) ttACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc < 1:323313/251‑1 (MQ=255) ttACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTCTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc < 1:438528/251‑1 (MQ=255) ttGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCg < 1:105586/251‑1 (MQ=255) ttGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCg < 1:833597/251‑1 (MQ=255) gAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGAccagtgtt > 1:401941/1‑245 (MQ=255) aTTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa < 1:145787/251‑1 (MQ=255) aTTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa < 1:775030/251‑1 (MQ=255) ccattttCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTtc < 1:390491/248‑1 (MQ=255) tttCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTcca > 1:462289/1‑251 (MQ=255) || GTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACTTTCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCA > 1/855401‑855754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |