| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 855,408 | 2 bp→AA | intergenic (‑5751/‑66809) | unknown ← / → unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 855,408 | 0 | G | A | 87.5% | 10.8 / ‑4.0 | 8 | intergenic (‑5751/‑66810) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (2/5); total (2/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
| * | 1 | 855,409 | 0 | T | A | 87.5% | 12.7 / ‑2.8 | 8 | intergenic (‑5752/‑66809) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (2/5); total (2/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACC > 1/855198‑855646 || gttgtcCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTa > 1:538473/1‑251 (MQ=255) gTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGc < 1:818760/250‑1 (MQ=255) tATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCAAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCt < 1:699117/251‑1 (MQ=255) aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACg < 1:794038/236‑1 (MQ=255) aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCtt > 1:604769/1‑251 (MQ=255) cATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGtacttact < 1:549885/237‑1 (MQ=255) ccAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTgccc < 1:322659/251‑4 (MQ=255) gTTAGTAGTCCGTGATTAATAAACTCGCCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCTTGCAAAACGATTTGACCGTTTTCCATTCCCATAACTTCAGTGATATTCATAATTTTTCTTGAACCATCGTTTAAGCGGGACGCCTGCACTATTATGTTTACTGCTGAAGAAATATTACGGCGAATTGCCTCCAACGGTAGGGATGCATTCGACATCATAAcc < 1:507049/246‑1 (MQ=255) || GTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACC > 1/855198‑855646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |