Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,121	2 bp→CT	intergenic (‑5464/‑67096)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,121	0	T	C	100.0%	50.1 / NA	19	intergenic (‑5464/‑67097)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/10);  total (9/10)
*	1	855,122	0	C	T	100.0%	46.5 / NA	19	intergenic (‑5465/‑67096)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/10);  total (9/10)

CACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGATCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTGCGAATCAAGTTTCTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAATTTTTGATTTGAGGCTCTTAAAAAAACTTGTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCA  >  1/854908‑855358                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                             cgctagAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGt                                                                                                                                                                                                                  >  1:484854/6‑243 (MQ=255)                                     ccTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAg                                                                                                                                                                                                                   <  1:255825/206‑1 (MQ=255)                                     ccTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTaaaaaaa                                                                                                                                                                      >  1:225040/1‑251 (MQ=255)                                                                     ttaCGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATagag                                                                                                                                                 <  1:420882/240‑1 (MQ=255)                                                                                    aGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTatag                                                                                                                                                   <  1:726634/223‑4 (MQ=255)                                                                                                            ccAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGt                                                                                               <  1:192908/251‑1 (MQ=255)                                                                                                             caacaaGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGta                                                                                              >  1:657485/1‑251 (MQ=255)                                                                                                                                  acacTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTaa                                                                                                                                                                                                                     >  1:785880/1‑111 (MQ=255)                                                                                                                                  acacTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATagag                                                                                                                                                 >  1:492030/1‑179 (MQ=255)                                                                                                                                            gtCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGtaa                                                                  <  1:414867/247‑3 (MQ=255)                                                                                                                                                atatGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATagag                                                                                                                                                 >  1:897551/1‑165 (MQ=255)                                                                                                                                                atatGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATaga                                                                                                                                                  <  1:604747/164‑1 (MQ=255)                                                                                                                                                    gAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATaga                                                                                                                                                  <  1:831896/160‑1 (MQ=255)                                                                                                                                                               aCATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATAc                                                                                                                                        <  1:482514/159‑1 (MQ=255)                                                                                                                                                               aCATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGtatat                                                                                           >  1:299468/1‑204 (MQ=255)                                                                                                                                                                        aCAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcac                                   <  1:795947/251‑11 (MQ=255)                                                                                                                                                                                                  acTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGt                                                                                               >  1:431997/1‑165 (MQ=255)                                                                                                                                                                                                     tataACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcactcagc                              <  1:660140/227‑16 (MQ=255)                                                                                                                                                                                                          cAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTaactcagta  >  1:84604/1‑242 (MQ=255)                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                             CACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGATCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTGCGAATCAAGTTTCTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAATTTTTGATTTGAGGCTCTTAAAAAAACTTGTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCA  >  1/854908‑855358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑