Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,387	2 bp→AC	intergenic (‑4730/‑67830)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,387	0	T	A	100.0%	75.3 / NA	30	intergenic (‑4730/‑67831)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/15);  total (15/15)
*	1	854,388	0	A	C	100.0%	79.5 / NA	30	intergenic (‑4731/‑67830)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/15);  total (15/15)

GCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTTTAATCATCATGTTGACTTCCTCATCAACCACCAAATGATTCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATT  >  1/854165‑854606                                                                                                                                                                                                                               ||                                                                                                                                                                                                                           gtaaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATaa                                                                                                                                                                                                 >  1:347992/18‑251 (MQ=255)  taaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAg                                                                                                                                                                                                <  1:761644/235‑1 (MQ=255)          taatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGacagtaat                                                                                                                                                                                        <  1:741339/243‑8 (MQ=255)                 taaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgct                                                                                                                                                                                    <  1:170225/247‑3 (MQ=255)                                                         tttaaaacgggATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAGTTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTccc                                                                                                                                         <  1:889899/242‑1 (MQ=255)                                                                  ggATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGt                                                                                                                                                                        <  1:318174/211‑1 (MQ=255)                                                                       attgttaatcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgc                                                                                                                                                                                     <  1:177660/189‑2 (MQ=255)                                                                       attgttaatcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAAt                                                                                                                                                <  1:258979/226‑1 (MQ=255)                                                                              atcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGac                                                                                                                                                                                              >  1:299930/4‑176 (MQ=255)                                                                              atcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCa                                                                                                                    >  1:343804/4‑251 (MQ=255)                                                                                  gtaacagctcaagttcaaTTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATAc                                                                                                                                     >  1:877369/9‑230 (MQ=255)                                                                                              gttcaaTTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCa                                                                                                    >  1:460298/1‑251 (MQ=255)                                                                                                                         tggctatttcatcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCattaatta                                                                         >  1:194400/20‑251 (MQ=255)                                                                                                                          ggctatttcatcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTc                                                                                                                                           <  1:172888/166‑1 (MQ=255)                                                                                                                                  catcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATaa                                                                                                                                                                                                        >  1:465881/11‑114 (MQ=255)                                                                                                                                    tcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAAc                                                                                                               <  1:791350/194‑1 (MQ=255)                                                                                                                                         actttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAAGTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGt                                                                                       <  1:439354/218‑1 (MQ=255)                                                                                                                                          ctttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGac                                                                                                                                                                                              <  1:143458/115‑2 (MQ=255)                                                                                                                                                    tAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCaa                                              <  1:746596/251‑1 (MQ=255)                                                                                                                                                       gTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCaaa                                             >  1:279608/1‑249 (MQ=255)                                                                                                                                                             ctcCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAAt                                                                                                                                                <  1:637747/144‑1 (MQ=255)                                                                                                                                                                  gTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTAc                                                                                           >  1:163611/1‑192 (MQ=255)                                                                                                                                                                  gTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGt                                                             >  1:719046/1‑222 (MQ=255)                                                                                                                                                                    aataatttctttcctatgctGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCattaa                                                                            >  1:496282/20‑205 (MQ=255)                                                                                                                                                                         tttctttcctatgctGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATaaa                                    <  1:693126/226‑1 (MQ=255)                                                                                                                                                                         tttctttcctatgctGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAaagaaaaagaaa                          >  1:273/15‑250 (MQ=255)                                                                                                                                                                              ttcctatgctGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAggg                                                                                   >  1:852618/10‑188 (MQ=255)                                                                                                                                                                                   atgctGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACg                                                                                                  >  1:606635/5‑168 (MQ=255)                                                                                                                                                                                          aaTTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCt        <  1:465374/251‑1 (MQ=255)                                                                                                                                                                                                cttCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGAtt  >  1:785683/2‑251 (MQ=255)                                                                                                                                                                                                                               ||                                                                                                                                                                                                                           GCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTTTAATCATCATGTTGACTTCCTCATCAACCACCAAATGATTCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATT  >  1/854165‑854606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑