Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,228	2 bp→CG	intergenic (‑4571/‑67989)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,228	0	T	C	100.0%	30.7 / NA	13	intergenic (‑4571/‑67990)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/6);  total (7/6)
*	1	854,229	0	A	G	100.0%	29.6 / NA	13	intergenic (‑4572/‑67989)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/6);  total (7/6)

GGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTT  >  1/854062‑854428                                                                                                                                                                       ||                                                                                                                                                                                                        ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCtt                                                                                                                      >  1:92602/1‑251 (MQ=255)                 ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTacctttggctatttcatcatt                                                                                                                                  >  1:450042/2‑203 (MQ=255)                 ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTtctc                                                                                                           >  1:611199/2‑246 (MQ=255)                            gTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTcttt                                                                                           >  1:282703/1‑248 (MQ=255)                                               gagaTGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAAtt                                                                                                                                                                     <  1:764065/158‑1 (MQ=255)                                                               aGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCACTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGcca                                                        >  1:643794/1‑249 (MQ=255)                                                                     atcaATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaata                                                  >  1:806403/1‑243 (MQ=255)                                                                                                        gtaaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATaa               >  1:347992/18‑251 (MQ=255)                                                                                                         taaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAg              <  1:761644/235‑1 (MQ=255)                                                                                                                 taatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGacagtaat      <  1:741339/243‑8 (MQ=255)                                                                                                                       gtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaataatac                                              <  1:738420/203‑13 (MQ=255)                                                                                                                        taaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgct  <  1:170225/247‑3 (MQ=255)                                                                                                                                               cAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCtatgc                                                                                    <  1:438670/143‑3 (MQ=255)                                                                                                                                                                       ||                                                                                                                                                                                                        GGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTT  >  1/854062‑854428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑