Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	820,707	C→A	intergenic (‑30/+28874)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	820,707	0	C	A	100.0%	90.7 / NA	35	intergenic (‑30/+28874)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/16);  total (19/16)

TCCCATCGGTTACTCTTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAGCCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGCTTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAATTCAACACGTGGAATTTCCGGTTTCTCATTCTATTTCGGAGCAGCTTATGC  >  1/820464‑820936                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      tCCCATCGGTTACTCTTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGAtttttt                                                                                                                                                                                                                                 <  1:568464/250‑1 (MQ=255)     aTCGGTTACTCTTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGc                                                                                                                                                                                                                            <  1:213254/251‑1 (MQ=255)      tCGGTTACTCTTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTGTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGcc                                                                                                                                                                                                                           <  1:434838/251‑1 (MQ=255)             ctctTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAg                                                                                                                                                                                                                    >  1:787097/1‑251 (MQ=255)             ctctTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAg                                                                                                                                                                                                                    >  1:662172/1‑251 (MQ=255)                         ggtggAGGGAGAAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACa                                                                                                                                                                                                                      >  1:882355/1‑237 (MQ=255)                                 gagaAGGATTCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAg                                                                                                                                                                                                >  1:672806/1‑251 (MQ=255)                                         ttCGAACCTTCGAAGGCTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATCCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTc                                                                                                                                                                                        <  1:386546/251‑1 (MQ=255)                                                         cTGAACCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAAtt                                                                                                                                                                        <  1:685977/251‑1 (MQ=255)                                                                    gATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGt                                                                                                                                                                                               >  1:288055/1‑217 (MQ=255)                                                                    gATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCaaacaaaa                                                                                                                                                             >  1:252710/1‑251 (MQ=255)                                                                                 cTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCtt                                                                                                                                                 <  1:758503/250‑1 (MQ=255)                                                                                   cccTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGt                                                                                                                                              >  1:838737/1‑251 (MQ=255)                                                                                    ccTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTc                                                                                                                                             <  1:210745/251‑1 (MQ=255)                                                                                              cTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATcaaac                                                                                                                                                                 >  1:339692/1‑221 (MQ=255)                                                                                                cGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGa                                                                                                                                 >  1:741137/1‑251 (MQ=255)                                                                                                              ctctAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGtt                                                                                                                                                                                              <  1:466266/176‑1 (MQ=255)                                                                                                              ctctAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTc                                                                                                                                                                                >  1:867232/1‑190 (MQ=255)                                                                                                                           cTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAAtct                                                                                                      <  1:652962/251‑1 (MQ=255)                                                                                                                                aTTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCttat                                                                                                 <  1:434779/251‑1 (MQ=255)                                                                                                                                  tGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCttattt                                                                                               <  1:163249/251‑1 (MQ=255)                                                                                                                                        aaaTGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCttatttat                                                                                             <  1:298869/247‑1 (MQ=255)                                                                                                                                                             tCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATAttt                                                                    <  1:744227/251‑1 (MQ=255)                                                                                                                                                                               gATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAAtt                                                   >  1:84399/1‑250 (MQ=255)                                                                                                                                                                               gATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAAtt                                                   >  1:437396/1‑250 (MQ=255)                                                                                                                                                                                 ttACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCtt                                                                                                                                                 <  1:846021/154‑1 (MQ=255)                                                                                                                                                                                                 ctacctacTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAg                                                                                          >  1:164265/1‑193 (MQ=255)                                                                                                                                                                                                          gAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGcc                                                                                                                                                                                                                           >  1:351909/1‑55 (MQ=255)                                                                                                                                                                                                          gAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACa                                                                                                                                                                                                                      >  1:521223/1‑60 (MQ=255)                                                                                                                                                                                                          gAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAATTCAACACGTGGAATTTCCGGTTTCTCAtt                       >  1:42221/1‑251 (MQ=255)                                                                                                                                                                                                                               cAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCttat                                                                                                 >  1:208797/1‑156 (MQ=255)                                                                                                                                                                                                                               cAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAATTCAacac                                             <  1:102398/208‑1 (MQ=255)                                                                                                                                                                                                                               cAAGTGACGCGTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAATTCAACACGTGGAATTTCCGGTTTCTCATTCTATTTCGGAGCAGCTTATGc  <  1:292041/251‑1 (MQ=255)                                                                                                                                                                                                                                         gTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCttatttat                                                                                             >  1:867207/1‑150 (MQ=255)                                                                                                                                                                                                                                         gTATTATAGGGATTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTgg                                                                  >  1:724437/1‑177 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      TCCCATCGGTTACTCTTAGATAATGGTGGAGGGAGAAGGATTCGAACCTTCGAAGGCTGAGCCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAATCCCTCCACTCTAAGTTTGAACTTGTATTGCGAGAAATGGTGCCGACTATCGGAATCGAACTGATGACCTACTGATTACAAGTCAGTTGCTCTACCTACTGAGCTAAGTCGGCGTCGAAAACAAGTGACGCGTATTATAGGGCTTTTTTCATGCCTGACAAGTTTTTTTTTAGTAAATAAAGTTTTTTTCAATTATTCGTTTAATTATCAAACAAAAACAATTTATCTTCGTCTAATTTACCTGATTATGCTAAATTTTTACTGCTCAATCTCTTATTTATAAGGTATATTTTGCAACCTATATTTGGCTAAAAAGAGTAATTCAACACGTGGAATTTCCGGTTTCTCATTCTATTTCGGAGCAGCTTATGC  >  1/820464‑820936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑