Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 788,497 T→C intergenic (+51375/+31796) unknown → / ← unknown tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1788,4970TC98.5% 183.7 / NA 67intergenic (+51375/+31796)unknown/unknowntRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (29/37);  minor base A (1/0);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GAAGTGGCATGGCAGGCGCATTTCGTGAAAAATATGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTATTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCGGATATTTATCGCGCA  >  1/788252‑788743
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
gAAGTGGCATGGCAGGCGCATTTCGTGAAAAATATGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTc                                                                                                                                                                                                                                                     >  1:64778/1‑249 (MQ=255)
                              aaTATGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTg                                                                                                                                                                                                                     <  1:423665/251‑1 (MQ=255)
                               atatGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCGGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGt                                                                                                                                                                                                                    <  1:586756/251‑1 (MQ=255)
                               atatGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGt                                                                                                                                                                                                                    <  1:343065/251‑1 (MQ=255)
                               atatGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGt                                                                                                                                                                                                                    <  1:584850/251‑1 (MQ=255)
                                 atGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGtt                                                                                                                                                                                                                   >  1:689222/1‑250 (MQ=255)
                                 atGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTc                                                                                                                                                                                                                  <  1:421553/251‑1 (MQ=255)
                                     ttATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCt                                                                                                                                                                                                              <  1:200234/251‑1 (MQ=255)
                                      tATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTa                                                                                                                                                                                                             <  1:590405/251‑1 (MQ=255)
                                                                        aaaCCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGc                                                                                                                                                                                                                         <  1:891174/205‑1 (MQ=255)
                                                                            cAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAGCTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGc                                                                                                                                                                       <  1:811573/251‑1 (MQ=255)
                                                                            cAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGc                                                                                                                                                                       <  1:521597/251‑1 (MQ=255)
                                                                             aaGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGTCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCt                                                                                                                                                                      <  1:120367/251‑1 (MQ=255)
                                                                                       gtgATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAgg                                                                                                                                                                                                                                       <  1:221818/176‑1 (MQ=255)
                                                                                       gtgATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCAc                                                                                                                                                                                                                       >  1:818029/1‑192 (MQ=255)
                                                                                         gatgaACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCg                                                                                                                                                          >  1:68677/1‑251 (MQ=255)
                                                                                            gaACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCggga                                                                                                                                                       >  1:379927/1‑249 (MQ=255)
                                                                                                 gTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTc                                                                                                                                                                                                                                                     >  1:183956/1‑152 (MQ=255)
                                                                                                 gTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAac                                                                                                                                                   >  1:92791/1‑250 (MQ=255)
                                                                                                 gTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAac                                                                                                                                                   >  1:153819/1‑250 (MQ=255)
                                                                                                 gTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGCTCTTCGGCTTGTCCCGCACCGGTAAAac                                                                                                                                                   >  1:578155/1‑250 (MQ=255)
                                                                                                 gTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGCGTTCTTCGGCTTGTCCGGCACCGGTAAAac                                                                                                                                                   >  1:133623/1‑250 (MQ=255)
                                                                                                  ttCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAacca                                                                                                                                                 <  1:789386/251‑1 (MQ=255)
                                                                                                          gCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCttt                                                                                                                                           >  1:730225/1‑249 (MQ=255)
                                                                                                          gCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGGAAAACCACCCttt                                                                                                                                           >  1:289885/1‑249 (MQ=255)
                                                                                                                              gAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTa                                                                                                                                                                                                             <  1:223825/163‑1 (MQ=255)
                                                                                                                              gAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAg                                                                                                                       >  1:401046/1‑249 (MQ=255)
                                                                                                                               aaCAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGc                                                                                                                      <  1:207072/249‑1 (MQ=255)
                                                                                                                                     gCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTgg                                                                                                               <  1:537253/250‑1 (MQ=255)
                                                                                                                                           aTTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGtgatgat                                                                                                        <  1:720823/251‑1 (MQ=255)
                                                                                                                                                 aaaaCTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAacac                                                                                                  <  1:858751/251‑1 (MQ=255)
                                                                                                                                                 aaaaCTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAacac                                                                                                  <  1:284778/251‑1 (MQ=255)
                                                                                                                                                    aCTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGt                                                                                               >  1:696811/1‑251 (MQ=255)
                                                                                                                                                    aCTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGt                                                                                               >  1:459289/1‑251 (MQ=255)
                                                                                                                                                      ttCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTg                                                                                             <  1:811887/251‑1 (MQ=255)
                                                                                                                                                            gCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCAc                                                                                                                                                            >  1:20511/1‑182 (MQ=255)
                                                                                                                                                            gCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGga                                                                                          >  1:71243/1‑248 (MQ=255)
                                                                                                                                                             cGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGgatg                                                                                        <  1:647686/249‑1 (MQ=255)
                                                                                                                                                                   acttaacCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTa                                                                                                                    <  1:577818/215‑1 (MQ=255)
                                                                                                                                                                      taacCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTTGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTAc                                                                                                                                       >  1:876605/1‑193 (MQ=255)
                                                                                                                                                                              cGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACtt                                                                     <  1:824829/251‑1 (MQ=255)
                                                                                                                                                                                    cAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAgg                                                               <  1:487770/251‑1 (MQ=255)
                                                                                                                                                                                                   aCCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGCTGAACTAATTCCCGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGAGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTTGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGATACGCGaa                                                >  1:896368/1‑251 (MQ=255)
                                                                                                                                                                                                   aCCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGAc                                                                                                                                                                                               <  1:760508/108‑1 (MQ=255)
                                                                                                                                                                                                   aCCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTAc                                                                                                                                       >  1:322145/1‑164 (MQ=255)
                                                                                                                                                                                                   aCCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGtgatgatg                                                                                                       >  1:601405/1‑196 (MQ=255)
                                                                                                                                                                                                              cggcgAAATGAAGAAAGGTATGTTCTCTATGATGAACTCCTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCAcc                                     <  1:458870/251‑1 (MQ=255)
                                                                                                                                                                                                              cggcgAAATGAAGAAAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCAcc                                     <  1:171792/251‑1 (MQ=255)
                                                                                                                                                                                                                       gaagaaAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAaca                                                                                                   <  1:88607/180‑1 (MQ=255)
                                                                                                                                                                                                                       gaagaaAGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGt                                                                                >  1:265735/1‑199 (MQ=255)
                                                                                                                                                                                                                            aaGGTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCAc                                                                                                                                                                                                                       <  1:276422/59‑1 (MQ=255)
                                                                                                                                                                                                                              ggTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGa                                                                                                                                                                                          <  1:397024/86‑1 (MQ=255)
                                                                                                                                                                                                                              ggTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAg                                                                <  1:533524/208‑1 (MQ=255)
                                                                                                                                                                                                                              ggTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGa                     >  1:408857/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGC‑TGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGaa                    >  1:223408/1‑251 (MQ=255)
                                                                                                                                                                                                                               gTATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCAcc                                     <  1:833271/234‑1 (MQ=255)
                                                                                                                                                                                                                                tATGTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAAc                   <  1:576573/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGc                                                                                                                                                                                                     >  1:42366/1‑70 (MQ=255)
                                                                                                                                                                                                                                   gTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATc                                                                                                                                  <  1:650296/137‑1 (MQ=255)
                                                                                                                                                                                                                                   gTTCTCTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCgg                >  1:249554/1‑251 (MQ=255)
                                                                                                                                                                                                                                   gTTCACTATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTAc                                                                                                                                       <  1:67459/132‑1 (MQ=255)
                                                                                                                                                                                                                                      ctctATGATGAACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACg                                                                                                                                                                                                          >  1:876197/1‑62 (MQ=255)
                                                                                                                                                                                                                                           tgatgaACTTCTTCCTGCCGCTAAAAGGCGTGGGCCCAATGCACTGTTCCGCTAACGTAGGCAAAGGCGGTGATGTTGCTGGGTTCTTCGGCTTGTCCGGCACCGGTAAAACCCCCCTTTCTTCCGATCCGGAACGTGAGCTAATTGGTGGTGATGAACGCGGTTGGGGTGGTGTGGGTATCGTCAAGTTTTAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCGGATATTTAt        <  1:57718/251‑1 (MQ=255)
                                                                                                                                                                                                                                           tgatgaACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCGGATATTTAt        <  1:415783/251‑1 (MQ=255)
                                                                                                                                                                                                                                               gaACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTa                                                                               >  1:246333/1‑176 (MQ=255)
                                                                                                                                                                                                                                               gaACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCAcc                                     >  1:754952/1‑218 (MQ=255)
                                                                                                                                                                                                                                               gaACTACTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCGGATATTTATcgcg    >  1:704471/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 actactTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGTAAACGAACCGGATATTTATCGCGCa  >  1:774769/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
GAAGTGGCATGGCAGGCGCATTTCGTGAAAAATATGTTTATTCGTCCGACCGACGCCGAATTAAAAGATTTCAAACCAAGTTTCGTGGTGATGAACGGTTCTAAATGCACTAACCCGAACTGGAAAGAACAAGGCTTGAATTCCGAAAACTTCGTAGCGTTTAACTTAACCGAACGCATTCAATTAATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGTATGTTCTCTATGATGAACTATTTCCTGCCGCTAAAAGGCGTGGGCGCAATGCACTGTTCCGCTAACGTAGGCAAAGACGGTGATGTTGCTGTGTTCTTCGGCTTGTCCGGCACCGGTAAAACCACCCTTTCTACCGATCCGAAACGTGAGCTAATTGGTGATGATGAACACGGTTGGGATGATGTGGGTATCTTCAACTTTGAAGGTGGTTGTTACGCGAAAACCATTCACCTTTCCGAAGAAAACGAACCGGATATTTATCGCGCA  >  1/788252‑788743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑