Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	787,626	A→G	intergenic (+50504/+32667)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	787,626	0	A	G	100.0%	150.0 / NA	56	intergenic (+50504/+32667)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

TTGTATAAAGGCACGTTAATGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGAGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGTAGTTAA  >  1/787402‑787866                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     ttgtATAAAGGCACGTTAATGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGaa                                                                                                                                                                                                                            <  1:153937/251‑1 (MQ=255)               gTTAATGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGtttt                                                                                                                                                                                                              >  1:891553/1‑251 (MQ=255)                  aaTGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTg                                                                                                                                                                                                           >  1:437466/1‑251 (MQ=255)                   aTGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTg                                                                                                                                                                                                           >  1:694940/1‑250 (MQ=255)                    tGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCg                                                                                                                                                                                                         <  1:185599/251‑1 (MQ=255)                               aTTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              <  1:552027/251‑1 (MQ=255)                               aTTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              <  1:341896/251‑1 (MQ=255)                                 tGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              <  1:106040/249‑1 (MQ=255)                                 tGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATag                                                                                                                                                                                            <  1:818873/251‑1 (MQ=255)                                  gTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              <  1:654191/248‑1 (MQ=255)                                  gTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATaga                                                                                                                                                                                           >  1:164353/1‑251 (MQ=255)                                                              gTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              >  1:105197/1‑220 (MQ=255)                                                              gTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCgggga                                                                                                                                                               >  1:709757/1‑249 (MQ=255)                                                                aCATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              <  1:457902/218‑1 (MQ=255)                                                                   tAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑ttt                                                                                                                                                         <  1:843111/251‑1 (MQ=255)                                                                            aTTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTTC‑CTaaaaa                                                                                                                                               >  1:316282/1‑250 (MQ=255)                                                                               aaaTAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGc                                                                                                                                                                                 <  1:809579/216‑1 (MQ=255)                                                                                  taatTGGAACTGTATTTGATGTTTAAACCGGCAAGGCCTGCAGCCCACGAGGCGGGAAAGTAAAGCAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTACA‑TTTACTCTaaattctaca                                                                                                                                          >  1:686219/1‑244 (MQ=255)                                                                                   aatTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTTC‑CTAAAATGCAATaa                                                                                                                                        >  1:531152/1‑251 (MQ=255)                                                                                        gAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGGAA‑TTTTC‑CTAAAAAGCAATaaaaaaa                                                                                                                                   >  1:785377/1‑250 (MQ=255)                                                                                           cTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGaa                                                                                                                                                                                                                            >  1:229651/1‑161 (MQ=255)                                                                                             gTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATag                                                                                                                                                                                            >  1:704086/1‑191 (MQ=255)                                                                                             gTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTGAA‑TTTTT‑CTAAAAATCAaaaaaaaaaaaatt                                                                                                                              >  1:251021/1‑249 (MQ=255)                                                                                             gTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGTAA‑TTTTC‑CTAAAATTAAAAAAAAAAAAAAg                                                                                                                               >  1:514587/1‑250 (MQ=255)                                                                                             gTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGCGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTTC‑ATAAAAAACAATaaaaaaaaaaaa                                                                                                                              >  1:488388/1‑249 (MQ=255)                                                                                                                    ggTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAata                                                                                                                  <  1:359184/240‑1 (MQ=255)                                                                                                                    ggTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAATTTTAC‑TTAAAAT‑CAATAAAAAAAAAAACAAAGTTCAAAAAttttattaaaa                                                                                                       >  1:275756/1‑243 (MQ=255)                                                                                                                     gTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAAAAAAAAATaaaaaaatggtt                                                                                                                       >  1:706831/1‑225 (MQ=255)                                                                                                                     gTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTTC‑CTAAAAAACAAAAAAAAAAAAAACaaaactggctctttttaacaatt                                                                                                       >  1:470834/1‑229 (MQ=255)                                                                                                                                             gtaaagtaaaGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTGAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAAAGTTCAAATATTTTATCAAACTAAC‑AGTTTTTTGGGAGAAAttttt                                                                             >  1:699921/1‑250 (MQ=255)                                                                                                                                             gtaaagtaaaGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGAAATGTTCAAATATTTGATCAAGCTAAA‑AGTTTTTTGGGAGAAAttttt                                                                             >  1:335800/1‑250 (MQ=255)                                                                                                                                                  gtaaagCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAata                                                                                                                  <  1:67454/210‑1 (MQ=255)                                                                                                                                                              gTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTaaa                                                                                                                                                                                                                                              <  1:604820/76‑1 (MQ=255)                                                                                                                                                              gTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAAt                                                                                                                                                                                              >  1:476718/1‑124 (MQ=255)                                                                                                                                                              gTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTTC‑CTAAAATGCAATAAAAAATAAAAAAATGTTTAAATATTTGATCAAGCTAAC‑AGTTTTTTTGGAGAAAATTTTATAGAATGTTGTTCAA‑t                                                           >  1:873779/1‑251 (MQ=255)                                                                                                                                                                      gTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAT‑CTAAAAAGCAATAAAAAAAAAAACAATGTTCAAAAATTTTATCAAACTAAC‑AGTTTTTTGGGAGAAATTTTCATAGAATGTTGTCCAA‑ttttttaat                                                   >  1:58320/1‑247 (MQ=255)                                                                                                                                                                      gTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTAT‑CTAAAATGCAATAAAAAATAAAAAAATGTTCAAATATTTTATCAAGCTAAC‑AATTTTTTGGGAGAAAATTTTATAGAATGTTGTTCAA‑tttttta                                                     >  1:354448/1‑247 (MQ=255)                                                                                                                                                                              tAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAacac                                           <  1:41338/251‑1 (MQ=255)                                                                                                                                                                              tAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAacac                                           <  1:706183/251‑1 (MQ=255)                                                                                                                                                                                aTTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAAtg                                                                     <  1:551249/224‑1 (MQ=255)                                                                                                                                                                                     aTGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAAAGCAATAAAAAAAAAAACAAAGTTTAAATATTTTATCAAGCTAAC‑AAGTTTTAGGGAGAAAATTTTATAGAATGTTGTCCCA‑TTTTTAACTTTTAACACAAAGAgg                                    >  1:525690/1‑251 (MQ=255)                                                                                                                                                                                        cTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGa                                 <  1:179922/251‑1 (MQ=255)                                                                                                                                                                                                  cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                       <  1:781274/251‑1 (MQ=255)                                                                                                                                                                                                  cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                       <  1:156300/251‑1 (MQ=255)                                                                                                                                                                                                  cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                       <  1:619885/251‑1 (MQ=255)                                                                                                                                                                                                  cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                       <  1:583014/251‑1 (MQ=255)                                                                                                                                                                                                   gggTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTAT‑CTAAAATGCAATAAAAAATAAAAAAATGTTCAAATATTTGATTAAGCTAAC‑AGttttttt                                                                                         >  1:88852/1‑183 (MQ=255)                                                                                                                                                                                                     gtggtTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAg                     <  1:167907/250‑1 (MQ=255)                                                                                                                                                                                                        gtTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGCAA‑TTTTT‑CTTAAATGTAATAAAAAAAAAAAAAAAGGTCAAAAATTTTATTAAGGTAAA‑AGATTTTAGGGAGAAAATTTTATAGAATGTTTTTCAA‑TTTTTTAATTTTTACAAAATGAGGGGAAGTATATGaaaaatat                 >  1:399784/1‑244 (MQ=255)                                                                                                                                                                                                            aTCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑Ct                                                                                                                                                    <  1:591626/118‑1 (MQ=255)                                                                                                                                                                                                            aTCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑ATTAC‑CCAAAATGCAATAAAAAATAAAACAATGTTCAAAAATTTTATCAAGACAACAAGTTTTT‑GGGAGAAAATTTTATAAAATGTTTTTCAA‑TTTTTTAATTTTAACACAAAGAGGGGAAGTATATGACAAAAGTaaaa             >  1:874142/1‑250 (MQ=255)                                                                                                                                                                                                             tCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGGAAA‑TTTTA‑CTAAAATGCAATAAAAAAAAAAACAAAGTTTAAAAATTTTATTAAGATAAA‑AGGTTTTAg                                                                                         >  1:768868/1‑175 (MQ=255)                                                                                                                                                                                                                    gtTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCt                                                                                                      <  1:738812/156‑1 (MQ=255)                                                                                                                                                                                                                       aCTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAacac                                           <  1:753275/210‑1 (MQ=255)                                                                                                                                                                                                                       aCTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAAAAAAACAATGTTCAAAAATTTTATCAAACTAAA‑AGTTTTTTGGGAGAAAATTTTATAAAAAGTTGTCCAATTTTTTAATTTTTAA‑AAAATGAGGGGAAGTATATGAAAGATGTAAAAAACGGAGTTaa  >  1:247489/1‑251 (MQ=255)                                                                                                                                                                                                                            gAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                       <  1:185701/225‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     TTGTATAAAGGCACGTTAATGTTAGCTTTAATTGTATATTCAAAGCCGTCCGATTTGAAATGTACATAAGCCGGCATTAAATAATTGGAACTGTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGAGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTAAA‑TTTAC‑CTAAAATGCAATAAAAAATAAAGCAATGTTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAGAAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGTAGTTAA  >  1/787402‑787866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑