Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	764,882	C→T	intergenic (+27760/+55411)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	764,882	0	C	T	100.0%	242.4 / NA	86	intergenic (+27760/+55411)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/34);  total (52/34)

ATCCTGTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGCCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAAGAGGTATTGTCAAA  >  1/764638‑765109                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    aTCCTGTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGttt                                                                                                                                                                                                                               >  1:418236/1‑251 (MQ=255) aTCCTGTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGttt                                                                                                                                                                                                                               >  1:149604/1‑251 (MQ=255) aTCCTGTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGttt                                                                                                                                                                                                                               >  1:842680/1‑251 (MQ=255) aTCCTGTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGttt                                                                                                                                                                                                                               >  1:220473/1‑251 (MQ=255)      gTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCGATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTc                                                                                                                                                                                                                            >  1:426626/1‑249 (MQ=255)       tACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTc                                                                                                                                                                                                                            >  1:515529/1‑248 (MQ=255)               tcGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCtt                                                                                                                                                                                                                 <  1:32093/251‑1 (MQ=255)                  ccGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTgg                                                                                                                                                                                                               <  1:90468/250‑1 (MQ=255)                        ggACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCAtttt                                                                                                                                                                                                        >  1:396896/1‑251 (MQ=255)                                         actcactGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTAt                                                                                                                                                                                       <  1:712440/251‑1 (MQ=255)                                           tcactGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATtg                                                                                                                                                                                     <  1:875695/251‑1 (MQ=255)                                            cactGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCacac                                                                                                                                                                                             >  1:689423/1‑242 (MQ=255)                                            cactGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATtgt                                                                                                                                                                                    >  1:229125/1‑251 (MQ=255)                                              ctGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTgtgg                                                                                                                                                                                  <  1:461961/251‑1 (MQ=255)                                                     agaaAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATtg                                                                                                                                                                                     >  1:181848/1‑241 (MQ=255)                                                             cAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGt                                                                                                                                                                   >  1:192066/1‑251 (MQ=255)                                                                    tCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            <  1:621311/251‑1 (MQ=255)                                                                    tCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            <  1:472568/251‑1 (MQ=255)                                                                      aaTTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:531445/1‑249 (MQ=255)                                                                           atttTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTgtggtg                                                                                                                                                                                >  1:198013/1‑224 (MQ=255)                                                                           atttTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            <  1:135360/244‑1 (MQ=255)                                                                           atttTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTAt                                                                                                                                                     >  1:369750/1‑251 (MQ=255)                                                                             ttttAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTc                                                                                                                                                   <  1:164340/251‑1 (MQ=255)                                                                              tttAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATtg                                                                                                                                                                                     <  1:429967/216‑1 (MQ=255)                                                                                      cGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTc                                                                                                                                                                                                                            >  1:732661/1‑169 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTcc                                                                                                                                                                                                                           <  1:814161/166‑1 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTAtt                                                                                                                                                                                      <  1:53034/203‑1 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTgtgg                                                                                                                                                                                  <  1:595756/207‑1 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGtat                                                                                                                                                                 >  1:578744/1‑224 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAgg                                                                                                                                                              <  1:194244/227‑1 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:882458/1‑229 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            <  1:368433/229‑1 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            <  1:326673/229‑1 (MQ=255)                                                                                          tCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:6099/1‑229 (MQ=255)                                                                                            aTTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTg                                                                                                                                    >  1:363728/1‑251 (MQ=255)                                                                                               ggATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCacac                                                                                                                                                                                             >  1:764051/1‑191 (MQ=255)                                                                                                            aaaaaaaTCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAgg                                                                                                                                                              <  1:570912/209‑1 (MQ=255)                                                                                                                 aaTCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAAc                                                                                                                                       <  1:764857/227‑1 (MQ=255)                                                                                                                         ccAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACaa                                                                                                                                        >  1:675100/1‑218 (MQ=255)                                                                                                                              atTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGtat                                                                                                                                                                 <  1:190075/188‑1 (MQ=255)                                                                                                                                  aaaTTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTCGCCATAGGAAATTTAAAAATtaat                                                                                              >  1:309100/1‑251 (MQ=255)                                                                                                                                      tGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGa                                                                                                             <  1:785571/232‑1 (MQ=255)                                                                                                                                           gTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAgg                                                                                                              >  1:438125/1‑226 (MQ=255)                                                                                                                                                     tctGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:590339/1‑170 (MQ=255)                                                                                                                                                                aTCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAg                                                                >  1:71549/1‑251 (MQ=255)                                                                                                                                                                aTCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAg                                                                >  1:729406/1‑251 (MQ=255)                                                                                                                                                                 tCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:633102/1‑158 (MQ=255)                                                                                                                                                                     gAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACg                                                           >  1:662666/1‑251 (MQ=255)                                                                                                                                                                          tAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACg                                                           <  1:701088/246‑1 (MQ=255)                                                                                                                                                                          tAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAg                                                      >  1:447506/1‑251 (MQ=255)                                                                                                                                                                                atcTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAAc                                                                                                                                       <  1:202097/164‑1 (MQ=255)                                                                                                                                                                                atcTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGa                                                 >  1:151343/1‑250 (MQ=255)                                                                                                                                                                                atcTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGAACGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            <  1:724883/143‑1 (MQ=255)                                                                                                                                                                                 tcTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGc                                                                                                                                                                      >  1:553807/1‑132 (MQ=255)                                                                                                                                                                                 tcTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTG‑TCGTCCGTTTTTCCTGatcatc                                                                                                                                                                                                                   <  1:764624/86‑1 (MQ=255)                                                                                                                                                                                  cTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:725810/1‑141 (MQ=255)                                                                                                                                                                                       aGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCcac                                                                                                                                                                                               >  1:660226/1‑101 (MQ=255)                                                                                                                                                                                       aGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGttt                                         >  1:830886/1‑251 (MQ=255)                                                                                                                                                                                        gCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGtttt                                        >  1:415294/1‑251 (MQ=255)                                                                                                                                                                                            atatTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAgg                                                                                                                                                              >  1:101553/1‑129 (MQ=255)                                                                                                                                                                                            atatTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGt                                                     >  1:550895/1‑234 (MQ=255)                                                                                                                                                                                            atatTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGAc                                                <  1:813319/239‑1 (MQ=255)                                                                                                                                                                                             tatTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCg                                   <  1:868333/251‑1 (MQ=255)                                                                                                                                                                                                 ttGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCatg                               >  1:215471/1‑251 (MQ=255)                                                                                                                                                                                                      aaTCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCg                                                                                                                                                  <  1:782098/131‑1 (MQ=255)                                                                                                                                                                                                      aaTCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGACATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCatgatg                            >  1:398285/1‑249 (MQ=255)                                                                                                                                                                                                           gATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTGCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCg                                                                                                                                                  <  1:712377/126‑1 (MQ=255)                                                                                                                                                                                                           gATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTc                                                                                                                                                            >  1:668226/1‑116 (MQ=255)                                                                                                                                                                                                           gATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGt                                                                        <  1:809431/200‑1 (MQ=255)                                                                                                                                                                                                           gATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGtt                                                              >  1:783213/1‑210 (MQ=255)                                                                                                                                                                                                           gATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGt                                                     >  1:492771/1‑219 (MQ=255)                                                                                                                                                                                                                aTGCACAGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGaa                >  1:631847/1‑251 (MQ=255)                                                                                                                                                                                                                     caGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAAc                                                                                                                                       <  1:133419/127‑1 (MQ=255)                                                                                                                                                                                                                     caGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGc                                                                                                                          <  1:755341/140‑1 (MQ=255)                                                                                                                                                                                                                     caGAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAAGAGGt           >  1:662058/1‑251 (MQ=255)                                                                                                                                                                                                                       gAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAgg                                                                                                                                                              >  1:856728/1‑102 (MQ=255)                                                                                                                                                                                                                       gAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTc                                                         >  1:646689/1‑203 (MQ=255)                                                                                                                                                                                                                       gAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAAGAGGTAt         >  1:829545/1‑251 (MQ=255)                                                                                                                                                                                                                       gAAAGAGATCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAAGAGGTAt         >  1:829569/1‑251 (MQ=255)                                                                                                                                                                                                                             gaTCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAag               <  1:794846/239‑1 (MQ=255)                                                                                                                                                                                                                              aTCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAAttt                                                                                                                                                                                                   <  1:9028/58‑1 (MQ=255)                                                                                                                                                                                                                              aTCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCATACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTtct                      <  1:433894/231‑1 (MQ=255)                                                                                                                                                                                                                              aTCCTAAACTACATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAAGAGGTATTGTCaaa  >  1:165627/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCt                                                                                                                                                                             >  1:129369/1‑70 (MQ=255)                                                                                                                                                                                                                                        aCATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTg                  >  1:339814/1‑225 (MQ=255)                                                                                                                                                                                                                                        aCATTTTTGATCGTCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAag               >  1:342649/1‑228 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    ATCCTGTACTAATCTCGCCGAGTGGACAAATTCTCGGCGCACTCACTGAAGAAGAAAAATCAGTACGTCAATTTATTTTTAACAACGCTTCATTGGATCAACCGTTAAAAAAAATCTATTCCAATATTCAAATTGTCGGTCCGTTTTCTCTGTACCTAAATCCTGAGCATAATCAATCTTATAGCTTATATTTTGTCAATCAGATCAATGCACAGAAAGAGATCCTAAACTACATTTTTGATCGCCCGTTTTTCCTGATCATCTTGGTCATTTTAATTTCCACACCGTTATTGTGGTGGCTATCACGCAGTATAGGTCAGCCTATTCGTAACCTACAACTTGCAGCAAATGCCGTTGCCATAGGAAATTTAAAAATTAATAAAAATCTTGAAACCAAAGGGTTGATCGAGTTACGTCAAGTAGGACAAAGTTTTAACCGCATGATGCTTTCTCTTGAAGAGGTATTGTCAAA  >  1/764638‑765109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑