Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	758,553	C→T	intergenic (+21431/+61740)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	758,553	0	C	T	100.0%	197.6 / NA	69	intergenic (+21431/+61740)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/40);  total (29/40)

AAAAATTGCAAAAACGCTGTGCCAAAGTCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTCCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAATGCCCAACACTTTACGCCCGTTGATGCTGG  >  1/758309‑758799                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       aaaaaTTGCAAAAACGCTGTGCCAAAGTCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGt                                                                                                                                                                                                                                                     <  1:248099/248‑1 (MQ=255)         cAAAAACGCTGTGCCAAAGTCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTagaaga                                                                                                                                                                                                                                          >  1:378102/1‑251 (MQ=255)             aaCGCTGTGCCAAAGTCGGCTTTGACTGGACAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGaaaaa                                                                                                                                                                                                                                      >  1:456908/1‑251 (MQ=255)                    tgCCAAAGTCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGc                                                                                                                                                                                                                               <  1:736813/251‑1 (MQ=255)                          aGTCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGCGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGCAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGcaacaa                                                                                                                                                                                                                          >  1:229299/1‑250 (MQ=255)                            tCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCaacaac                                                                                                                                                                                                                         <  1:808071/249‑1 (MQ=255)                            tCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACAt                                                                                                                                                                                                                       <  1:657677/251‑1 (MQ=255)                               gCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCg                                                                                                                                                                                                                     <  1:354818/250‑1 (MQ=255)                                      cTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAGGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTgg                                                                                                                                                                                                             >  1:428327/1‑251 (MQ=255)                                         gAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAg                                                                                                                                                                                                          >  1:442767/1‑251 (MQ=255)                                                aTTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGtc                                                                                                                                                                                                   <  1:669150/251‑1 (MQ=255)                                                       cGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTc                                                                                                                                                                                            <  1:434092/251‑1 (MQ=255)                                                           ttttGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGC‑AGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAAt                                                                                                                                                                                       <  1:3966/251‑1 (MQ=255)                                                                  aaaGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGAt                                                                                                                                                                                   >  1:414695/1‑249 (MQ=255)                                                                     gTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTaga                                                                                                                                                                                                                                             >  1:795972/1‑188 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAAt                                                                                                                                                                                       >  1:636736/1‑235 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:210753/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:250243/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:204215/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:340348/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:186308/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:636370/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:864738/250‑1 (MQ=255)                                                                            agGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:88356/250‑1 (MQ=255)                                                                                 ttACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGAtgaagt                                                                                                                                                                  >  1:251253/1‑251 (MQ=255)                                                                                          gTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGt                                                                                                                                                                                                                                                     >  1:732934/1‑159 (MQ=255)                                                                                          gTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTc                                                                                                                                                                                                                  >  1:683658/1‑194 (MQ=255)                                                                                          gTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGAt                                                                                                                                                                       <  1:662313/237‑1 (MQ=255)                                                                                             aaaCAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGgaaga                                                                                                                                                      <  1:656053/251‑1 (MQ=255)                                                                                                  ggAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGaaaaa                                                                                                                                                  >  1:52028/1‑250 (MQ=255)                                                                                                             gCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTaaaa                                                                                                                                       >  1:140487/1‑250 (MQ=255)                                                                                                              ctctCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTa                                                                                                                                                                             <  1:608219/211‑1 (MQ=255)                                                                                                              ctctCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAAc                                                                                                                                      <  1:247562/250‑1 (MQ=255)                                                                                                              ctctCCACAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATc                                                                                                                                                                                  >  1:802172/1‑206 (MQ=255)                                                                                                                ctcCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:743550/214‑1 (MQ=255)                                                                                                                  ccGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATc                                                                                                                                                                                  <  1:663402/202‑1 (MQ=255)                                                                                                                        gATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACtt                                                                                                                           >  1:500272/1‑251 (MQ=255)                                                                                                                         aTCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACttt                                                                                                                          >  1:618708/1‑251 (MQ=255)                                                                                                                            aaCAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTcc                                                                                                                       <  1:711736/251‑1 (MQ=255)                                                                                                                                          gaagaaATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAAcc                                                                                                                                     >  1:315259/1‑223 (MQ=255)                                                                                                                                             gaaATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        <  1:173564/185‑1 (MQ=255)                                                                                                                                             gaaATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        >  1:245700/1‑185 (MQ=255)                                                                                                                                             gaaATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGga                                                                                                                                                         <  1:543370/200‑1 (MQ=255)                                                                                                                                             gaaATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGaaaa                                                                                                      >  1:102508/1‑251 (MQ=255)                                                                                                                                                   ggCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTg                                                                                                >  1:768993/1‑251 (MQ=255)                                                                                                                                                           gTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGt                                                                                                                                                                                                                                                     <  1:534709/94‑1 (MQ=255)                                                                                                                                                           gTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCa                                                                                          >  1:521809/1‑249 (MQ=255)                                                                                                                                                                ttGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGt                                                                                                                                                                                                                    <  1:781189/122‑1 (MQ=255)                                                                                                                                                                   cTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGc                                                                                 <  1:632331/250‑1 (MQ=255)                                                                                                                                                                    tACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGccc                                                                               <  1:299604/251‑1 (MQ=255)                                                                                                                                                                           aaTTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTG‑CCAATCAAAg                                                                       <  1:19111/251‑1 (MQ=255)                                                                                                                                                                            aTTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAGGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAg                                                                       >  1:437489/1‑251 (MQ=255)                                                                                                                                                                              ttAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGAt                                                                     <  1:708705/251‑1 (MQ=255)                                                                                                                                                                              ttAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGAt                                                                     <  1:643453/251‑1 (MQ=255)                                                                                                                                                                              ttAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGAt                                                                     <  1:487965/251‑1 (MQ=255)                                                                                                                                                                                   ccGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCg                                                                 <  1:479386/250‑1 (MQ=255)                                                                                                                                                                                                 gTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGa                                                                                                                                                                        >  1:76660/1‑133 (MQ=255)                                                                                                                                                                                                      gCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTAtttt                                             >  1:735323/1‑251 (MQ=255)                                                                                                                                                                                                      gCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTAtttt                                             >  1:61382/1‑251 (MQ=255)                                                                                                                                                                                                         gaagaaAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAAccc                                                                                                                                    <  1:314477/161‑1 (MQ=255)                                                                                                                                                                                                         gaagaaAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTAttttt                                            >  1:836961/1‑249 (MQ=255)                                                                                                                                                                                                               aGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGaaaaa                                                                                                                                                                                                                                      <  1:616992/57‑1 (MQ=255)                                                                                                                                                                                                                gCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAac                                                                                                                                                 <  1:829180/141‑1 (MQ=255)                                                                                                                                                                                                                    aaGCAAAGCGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAAt                               >  1:357813/1‑251 (MQ=255)                                                                                                                                                                                                                            cGAACCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAATGCCCAac                        <  1:120961/250‑1 (MQ=255)                                                                                                                                                                                                                              aaCCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAATGCCCAacac                      <  1:443936/250‑1 (MQ=255)                                                                                                                                                                                                                              aaCCAAAAATTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAATGCCCAacac                      <  1:207497/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaTTTGAACAACGCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTtttctttc                                       >  1:129711/1‑226 (MQ=255)                                                                                                                                                                                                                                                 gCTTTCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAATGCCCAACACTTTACGCCCGTTGATGCTgg  >  1:438922/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       AAAAATTGCAAAAACGCTGTGCCAAAGTCGGCTTTGACTGGAAAGACATTGCACCGGTTTTTGCCAAAGTGGAAGAGGAATTACAGGAAGTGAAACAGGAAATGGAACGCTCTCCGCAAGATCAACAGAAAATCGAAGAAGAAATGGGCGATTTGTTATTTGCTACCGTCAATTTAAGCCGTCATTTGAAATGTCAGGCGGAAGAAAGCCTAAGCAAAGCGAACCAAAAATTTGAACAACGCTTCCGTCGCGTAGAAGAAAAATTAAAGCAACAACATCGTTCTTTGGAAGACGCGTCTCTGGTCGAAATGGATCTGTTATGGGATGAAGTGAAGCAGGAAGAAAAACACCGCTAAAACCCACCGCACTTTTCCTTTAAAAAACACGAAAAAAATTGACCGCACTTTGTTGCCCAATCAAAGATACCGAGCGCTAAACTCGGTATTTTTCTTTCTAATCAATGCCCAACACTTTACGCCCGTTGATGCTGG  >  1/758309‑758799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑