Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	755,400	T→C	intergenic (+18278/+64893)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	755,400	0	T	C	100.0%	208.6 / NA	77	intergenic (+18278/+64893)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/41);  total (36/41)

TCCAGCTCCATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGTGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATGGTATCTACTGG  >  1/755166‑755632                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         tCCAGCTCCATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGGGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCAc                                                                                                                                                                                                                          <  1:206890/251‑1 (MQ=255) tCCAGCTCCATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCAc                                                                                                                                                                                                                          <  1:9693/251‑1 (MQ=255) tCCAGCTCCATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCAc                                                                                                                                                                                                                          <  1:644756/251‑1 (MQ=255)        ccATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCAt                                                                                                                                                                                                                     <  1:70318/249‑1 (MQ=255)         cATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCa                                                                                                                                                                                                                  >  1:506216/1‑251 (MQ=255)            aataTCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACgg                                                                                                                                                                                                               <  1:294/251‑1 (MQ=255)             atatCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGa                                                                                                                                                                                                              >  1:239484/1‑251 (MQ=255)                   gTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGAc                                                                                                                                                                                                             >  1:140496/1‑246 (MQ=255)                   gTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGATCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGcc                                                                                                                                                                                                         >  1:38423/1‑250 (MQ=255)                        aaTCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACgg                                                                                                                                                                                                   >  1:401422/1‑251 (MQ=255)                            gggTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATaa                                                                                                                                                                                               >  1:166894/1‑251 (MQ=255)                                   tCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCt                                                                                                                                                                                        <  1:771670/251‑1 (MQ=255)                                    cGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGAc                                                                                                                                                                                                     <  1:652053/237‑1 (MQ=255)                                     gCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTg                                                                                                                                                                                      >  1:774856/1‑251 (MQ=255)                                     gCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTg                                                                                                                                                                                      >  1:697653/1‑251 (MQ=255)                                        ctAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGt                                                                                                                                                                                   <  1:358618/251‑1 (MQ=255)                                              tGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGtt                                                                                                                                                                              >  1:860752/1‑250 (MQ=255)                                              tGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTc                                                                                                                                                                             <  1:764727/251‑1 (MQ=255)                                               gCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGtt                                                                                                                                                                              >  1:891803/1‑249 (MQ=255)                                                  gATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCg                                                                                                                                                                         >  1:285674/1‑251 (MQ=255)                                                   aTTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCg                                                                                                                                                                         <  1:111318/250‑1 (MQ=255)                                                       aCTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGAc                                                                                                                                                                                                     <  1:51015/218‑1 (MQ=255)                                                        cTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGa                                                                                                                                                                   >  1:900944/1‑251 (MQ=255)                                                          aTGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGAc                                                                                                                                                                  >  1:491286/1‑250 (MQ=255)                                                              gTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTg                                                                                                                                                                                                                        >  1:654106/1‑192 (MQ=255)                                                              gTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCt                                                                                                                                                                                        <  1:262250/224‑1 (MQ=255)                                                              gTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGCGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAAt                                                                                                                                                             >  1:3267/1‑251 (MQ=255)                                                                   aCTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTg                                                                                                                                                        <  1:86271/251‑1 (MQ=255)                                                                                gcgcTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGAc                                                                                                                                                                                                     <  1:756668/193‑1 (MQ=255)                                                                                 cgcTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTggg                                                                                                                                          <  1:56721/251‑1 (MQ=255)                                                                                     ttACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAAt                                                                                                                                                             >  1:776436/1‑228 (MQ=255)                                                                                       aCTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCtt                                                                                                                                    >  1:125566/1‑251 (MQ=255)                                                                                       aCTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCtt                                                                                                                                    >  1:568863/1‑251 (MQ=255)                                                                                          gATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTa                                                                                                                                 >  1:794255/1‑251 (MQ=255)                                                                                            tAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACg                                                                                                                               <  1:184452/251‑1 (MQ=255)                                                                                              ggTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAAcc                                                                                                                                                                                                                            <  1:559033/156‑1 (MQ=255)                                                                                              ggTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGAc                                                                                                                                                                                                     >  1:61497/1‑179 (MQ=255)                                                                                              ggTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATAcc                                                                                                                                                          >  1:372673/1‑222 (MQ=255)                                                                                              ggTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTggg                                                                                                                                          <  1:56511/238‑1 (MQ=255)                                                                                              ggTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGt                                                                                                                              >  1:670862/1‑250 (MQ=255)                                                                                                               gTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTggg                                                                                                                                          <  1:243394/221‑1 (MQ=255)                                                                                                                     aCGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTAc                                                                                                                                <  1:668688/225‑1 (MQ=255)                                                                                                                        cAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCAc                                                                                                                                                                           <  1:89442/179‑1 (MQ=255)                                                                                                                        cAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAg                                                                                                    >  1:280525/1‑250 (MQ=255)                                                                                                                                cATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATg                                                                                           >  1:672323/1‑251 (MQ=255)                                                                                                                                         cAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTggg                                                                                                                                          >  1:612122/1‑195 (MQ=255)                                                                                                                                         cAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGAtt                                                                                  <  1:283245/251‑1 (MQ=255)                                                                                                                                               aTCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGacc                                                                             <  1:705984/250‑1 (MQ=255)                                                                                                                                                   tttCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCg                                                                                                                                                                <  1:127353/163‑1 (MQ=255)                                                                                                                                                   tttCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAAcc                                                                                                                 <  1:642367/210‑1 (MQ=255)                                                                                                                                                   tttCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCgg                                                                        <  1:289123/251‑1 (MQ=255)                                                                                                                                                                  ttCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTTGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAAt                                                          <  1:423433/250‑1 (MQ=255)                                                                                                                                                                     gCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAg                                                                                                    <  1:808772/205‑1 (MQ=255)                                                                                                                                                                     gCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCAt                                                               <  1:584542/242‑1 (MQ=255)                                                                                                                                                                      ccTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTCATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGCCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGAtt                                                                                  >  1:448916/1‑222 (MQ=255)                                                                                                                                                                      ccTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGata                                                                                                                                                                      <  1:379030/138‑1 (MQ=255)                                                                                                                                                                      ccTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGAcc                                                                                                                                                     <  1:735554/155‑1 (MQ=255)                                                                                                                                                                      ccTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGt                                                     <  1:789910/251‑1 (MQ=255)                                                                                                                                                                           gACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGtattat                                                >  1:851378/1‑251 (MQ=255)                                                                                                                                                                                gACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATAtt                                                                                                                                                <  1:417676/150‑1 (MQ=255)                                                                                                                                                                                gACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTaaa                                           >  1:115394/1‑251 (MQ=255)                                                                                                                                                                                gACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTaaa                                           >  1:886532/1‑251 (MQ=255)                                                                                                                                                                                 aCGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTaaaa                                          >  1:598942/1‑251 (MQ=255)                                                                                                                                                                                           gcgTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCg                                 >  1:49637/1‑250 (MQ=255)                                                                                                                                                                                                  acaaATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGa                         >  1:167351/1‑251 (MQ=255)                                                                                                                                                                                                   caaATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGAc                        <  1:151772/251‑1 (MQ=255)                                                                                                                                                                                                   caaATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGAc                        <  1:665581/251‑1 (MQ=255)                                                                                                                                                                                                        gggTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGtat                                                   <  1:776528/219‑1 (MQ=255)                                                                                                                                                                                                          gTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGaa                   >  1:579059/1‑249 (MQ=255)                                                                                                                                                                                                          gTTGCAGAGTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAAc                  <  1:400959/250‑1 (MQ=255)                                                                                                                                                                                                                 agTGATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATg             <  1:709265/248‑1 (MQ=255)                                                                                                                                                                                                                    gATTTAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATGGTATc        >  1:782127/1‑250 (MQ=255)                                                                                                                                                                                                                      tttAATGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATGGTATCTAc     <  1:653230/251‑1 (MQ=255)                                                                                                                                                                                                                         aaTGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATGGTATCTACTgg  <  1:861681/251‑1 (MQ=255)                                                                                                                                                                                                                          aTGAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGt                                                     >  1:860765/1‑199 (MQ=255)                                                                                                                                                                                                                            gAAATACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAAc                                                                                                                  <  1:277124/136‑1 (MQ=255)                                                                                                                                                                                                                              aaTACGTTGACGGCGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATGGTa          >  1:615302/1‑238 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         TCCAGCTCCATAATATCCGTTTTAATCGGGTTATTCGCTCTAACTTGCTGATTAACTATGCGTTCTACTTCGGCTAATTGCGCTTTACTGATAGGTTCATGTTGAGCAAAGTCAAAACGCAATAATGCATCAGAAACAAGCGATCCTTTCTGAGCGACGTGTTCGCCTAAGACCTGACGTAACGCGGCGTGCAACAAATGGGTTGCAGAGTGATTTAATGAAATACGTTGACGGTGAACCTCGTCAACCACTGCATTCACGGACTGCCCGACGGATAAACTACCTTGGGTAAGTTCACCGATATGACCGAATACCTGACCATATTTTTGGGTGTCTTTTACGTCAAAATTAAAACCGTTGCCTTCTAAGCGCCCGATGTCTCCGATTTGACCACCGGATTCCGCATAGAATGGCGTATTATCTAAAATCACCACCGCACTTTGACCGGAACTGATGGTATCTACTGG  >  1/755166‑755632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑