Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	753,771	G→C	intergenic (+16649/+66522)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	753,771	0	G	C	100.0%	179.4 / NA	66	intergenic (+16649/+66522)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (38/28);  total (38/28)

GTTTTAAATTGCGCTTCAATCGCATCAATCACGTGCCCTGCGGCGCCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCAGGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGATAATCGTTTTGGGCGTTAAAAACTGCC  >  1/753525‑754005                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           gttttAAATTGCGCTTCAATCGCATCAATCACGTGCCCTGCGGCGCCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCa                                                                                                                                                                                                                                         >  1:604649/1‑250 (MQ=255)                      gCATCAATCACGTGCCCTGCGGCGCCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGt                                                                                                                                                                                                                    >  1:222596/1‑250 (MQ=255)                       cATCAATCACGTGCCCTGCGGCGCCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTcc                                                                                                                                                                                                                  <  1:750729/251‑1 (MQ=255)                              caCGTGCCCTGCGGCGCCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGt                                                                                                                                                                                                           <  1:481279/251‑1 (MQ=255)                                             gcCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCgggca                                                                                                                                                                                            >  1:75723/1‑248 (MQ=255)                                                 ttACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTc                                                                                                                                                                                        <  1:825400/251‑1 (MQ=255)                                                       gAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCCGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTTAGCCGTTGTAGTCCATCGGGGTGTGGCTTTCGGTCACTTCCATac                                                                                                                                                                                   >  1:729100/1‑250 (MQ=255)                                                             aTCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTTAGCCGTTGTAGTCCATCGGGTTGTGGc                                                                                                                                                                                                     >  1:642061/1‑226 (MQ=255)                                                                     cTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGGTTTCa                                                                                                                                                                    >  1:545533/1‑251 (MQ=255)                                                                             gTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAatga                                                                                                                                                             <  1:176619/250‑1 (MQ=255)                                                                                    cGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCaa                                                                                                                                                     <  1:87008/251‑1 (MQ=255)                                                                                            gaTTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGGTGTGGCTTGCGGTCACTTCAATac                                                                                                                                                                                   >  1:600141/1‑213 (MQ=255)                                                                                            gaTTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAACCGGACGGGACCCCTCACGCACTAATTTTTAGCCGTTGTAGTCCATCGGGGTGTGGCTTGCGGGCACTTCAATAACACCGCCAGTTTTTAAAAAAGATGTAGCAAAATAGa                                                                                                                                               >  1:545888/1‑249 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCCGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACCGGACCCCTCACGCACCAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGGCACTTCAATAACACCGGCAGTTTTTAAAAATGATGTAGCAAAATAAAATTCct                                                                                                                                         >  1:11305/1‑250 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTc                                                                                                                                                                                                                   >  1:758925/1‑176 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTTAAAAAAGATGTAGCAAAATAGACTTCct                                                                                                                                         >  1:573711/1‑250 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCctc                                                                                                                                        <  1:261379/251‑1 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAAAAGACTTCct                                                                                                                                         >  1:311776/1‑250 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGGCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCct                                                                                                                                         >  1:37582/1‑250 (MQ=255)                                                                                                 atatAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATacc                                                                                                                                                                                  >  1:608568/1‑209 (MQ=255)                                                                                                    taagataagaGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGGCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCctcccg                                                                                                                                     >  1:538298/1‑248 (MQ=255)                                                                                                       ggtaagaGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGGTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTTAAAAATGATGTAGCAAAATAGACTTCCTCTGTcc                                                                                                                                   >  1:105222/3‑250 (MQ=255)                                                                                                       gataagaGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGtt                                                                                                                                                                                                          <  1:804596/179‑1 (MQ=255)                                                                                                       gataagaGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTcc                                                                                                                                   >  1:610688/1‑250 (MQ=255)                                                                                                       gataagaGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTTAGCCGTTTTAGTCCATCGGGGTGTGGCTTGCGGTCACCTCAATTCCACCGTCCGGTTTCCAAAATGATGTTGCaaaaaaagcctcctccgtcc                                                                                                                                   >  1:521278/1‑234 (MQ=255)                                                                                                       gataagaGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCCCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCCCTTTAATaccac                                                                                                                                                                                >  1:756431/1‑205 (MQ=255)                                                                                                               gTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGc                                                                                                                                                                                                                             >  1:624163/1‑152 (MQ=255)                                                                                                               gTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGGTGTGGCTTGCGGGCACTTCAATacc                                                                                                                                                                                  >  1:503582/1‑195 (MQ=255)                                                                                                                 gCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCggg                                                                                                                                                                                                            >  1:65097/1‑167 (MQ=255)                                                                                                                    cAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCg                                                                                                                                                                              <  1:618792/194‑1 (MQ=255)                                                                                                                    cAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGGTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAACCGTTGTAGTCCATCGGGGTGTGGCTTTCGGGCACTTCAATACCCCCGTCAGTTTTTAAAAATGATTTAGCAAAATAGACTTCCTCTGTCCCCCCTTTACCTaaa                                                                                                                     >  1:181530/1‑251 (MQ=255)                                                                                                                              atcaAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATTCCACCGTCAGTTATCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACAt                                                                                                           <  1:614369/251‑1 (MQ=255)                                                                                                                              atcaAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGGATCCGCGCTAATCGGACGGGACCCCTCCCGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCCTCAGTTTTTAAAAATGATGTAGCAAAATAGACTTTCCCTGTCCCCCTTTTACCTAAATCAATTACAt                                                                                                           >  1:656437/1‑251 (MQ=255)                                                                                                                                    tACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGGTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAacac                                                                                                     <  1:733066/251‑1 (MQ=255)                                                                                                                                     aCGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATac                                                                                                                                                                                   <  1:825904/172‑1 (MQ=255)                                                                                                                                          aaGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGt                                                                                                                                                                                                           <  1:868936/143‑1 (MQ=255)                                                                                                                                          aaGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAAt                                                                                               <  1:58702/251‑1 (MQ=255)                                                                                                                                              tgttTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTccc                                                                                                                                  <  1:416106/212‑1 (MQ=255)                                                                                                                                                 ttATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCaataa                                                                                        <  1:786361/251‑1 (MQ=255)                                                                                                                                                        cgccgcGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCgg                                                                                                                                                                                               <  1:110894/141‑1 (MQ=255)                                                                                                                                                        cgccgcGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCctc                                                                                                                                        >  1:556605/1‑196 (MQ=255)                                                                                                                                                          ccgcGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTg                                                                                <  1:337140/250‑1 (MQ=255)                                                                                                                                                             cgTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGGCACTTTAATACCACCGTCAGTTTTTAAAAATGATGTAGc                                                                                                                                                       >  1:241836/1‑176 (MQ=255)                                                                                                                                                               tttGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGGCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCCTTTTACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTAcc                                                                           >  1:688148/1‑250 (MQ=255)                                                                                                                                                                          gcggGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGC‑CTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAAc                                                               <  1:565140/250‑1 (MQ=255)                                                                                                                                                                             ggAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAActc                                                             <  1:861164/250‑1 (MQ=255)                                                                                                                                                                                  ttattTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCaaa                                                                                                                                                                  >  1:682833/1‑144 (MQ=255)                                                                                                                                                                                    attTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCg                                                     <  1:443188/251‑1 (MQ=255)                                                                                                                                                                                      tttCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGtt                                                   <  1:777345/251‑1 (MQ=255)                                                                                                                                                                                         cctccGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAg                                                <  1:264507/251‑1 (MQ=255)                                                                                                                                                                                                   aCGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATcc                                                                                     >  1:389602/1‑204 (MQ=255)                                                                                                                                                                                                   aCGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTg                                                                                <  1:672391/209‑1 (MQ=255)                                                                                                                                                                                                   aCGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACAt                                         <  1:814671/248‑1 (MQ=255)                                                                                                                                                                                                          atatCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATcc                                                                                     >  1:237196/1‑197 (MQ=255)                                                                                                                                                                                                          atatCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCaa                                >  1:47957/1‑250 (MQ=255)                                                                                                                                                                                                             tCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAAAATCGCCACCAACGaa                            >  1:313894/1‑250 (MQ=255)                                                                                                                                                                                                                   aaCCCGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGACAATCGt                      >  1:40776/1‑251 (MQ=255)                                                                                                                                                                                                                     cccGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAg                                                <  1:272582/223‑1 (MQ=255)                                                                                                                                                                                                                     cccGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAg                                                >  1:255188/1‑223 (MQ=255)                                                                                                                                                                                                                       cGGTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGACAATCGTTTTg                  <  1:270080/251‑1 (MQ=255)                                                                                                                                                                                                                        ggTATCCGCGCTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGACAATCGTTTTgg                 >  1:581492/1‑251 (MQ=255)                                                                                                                                                                                                                               gcgcTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTcc                                                                                                                                                                                                                  >  1:873480/1‑51 (MQ=255)                                                                                                                                                                                                                               gcgcTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCt                                                                                                                        <  1:529507/141‑1 (MQ=255)                                                                                                                                                                                                                                cgcTAATCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCaataa                                                                                        <  1:308163/172‑1 (MQ=255)                                                                                                                                                                                                                                    aaTCGGACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGACAATCGTTTTGGGc               >  1:608917/1‑241 (MQ=255)                                                                                                                                                                                                                                        ggACGGGACCCTTCACGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGACAATCGTTTTGGGCGTTAAAAACTGcc  >  1:63538/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GTTTTAAATTGCGCTTCAATCGCATCAATCACGTGCCCTGCGGCGCCATTACCTGAGTTAATCACTAACTTCAACGGTTTTAGCGAATCGAGATTAATATAAGATAAGAGGTGCTCAACATAATCATCAAGTACGGAAAGTTGTTTATACTCGCCGCGTTTGGTCACCGGCGGGAAATTATTTTCCTCCGCCAGACGTTGAATATCCGCCAACCCGGTATCCGCGCTAATCGGACGGGACCCTTCAGGCACTAATTTTAAGCCGTTGTAGTCCATCGGGTTGTGGCTTGCGGTCACTTCAATACCACCGTCAGTTTTCAAAAATGATGTAGCAAAATAGACTTCCTCTGTCCCCGTTTCACCTAAATCAATTACATTAACACCTGAATCCAATAATCCATTTGTTACCGCACTTTTTAACTCTTTACTCGTTAAGCGAACATCGCCACCAACGATAATCGTTTTGGGCGTTAAAAACTGCC  >  1/753525‑754005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑