Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	745,226	G→T	intergenic (+8104/+75067)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	745,226	0	G	T	100.0%	205.1 / NA	75	intergenic (+8104/+75067)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/29);  total (46/29)

CGGTTAAATCAGGTGACGCAGGGTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGAGCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGACGGCGTGCGTTTTTCCCAT  >  1/744981‑745463                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               cGGTTAAATCAGGTGACGCAGGGTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGTGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGt                                                                                                                                                                                                                                           <  1:447918/251‑1 (MQ=255) cGGTTAAATCAGGTGACGCAGGGTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGt                                                                                                                                                                                                                                           <  1:679345/251‑1 (MQ=255) cGGTTAAATCAGGTGACGCAGGGTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGt                                                                                                                                                                                                                                           <  1:673919/251‑1 (MQ=255)            ggTGACGCAGGGTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTAt                                                                                                                                                                                                                                 >  1:532978/1‑250 (MQ=255)                       gTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCAtt                                                                                                                                                                                                                     >  1:613452/1‑251 (MQ=255)                         gCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTcg                                                                                                                                                                                                                   >  1:377732/1‑251 (MQ=255)                         gCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTcg                                                                                                                                                                                                                   >  1:189594/1‑251 (MQ=255)                           cTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTcgcg                                                                                                                                                                                                                 >  1:603714/1‑251 (MQ=255)                            ttGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGt                                                                                                                                                                                                                <  1:93605/251‑1 (MQ=255)                              ggATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGGCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTa                                                                                                                                                                                                               >  1:223795/1‑250 (MQ=255)                              ggATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCgg                                                                                                                                                                                                                                      >  1:636962/1‑227 (MQ=255)                              ggATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTa                                                                                                                                                                                                               >  1:689896/1‑250 (MQ=255)                              ggATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTa                                                                                                                                                                                                               >  1:615162/1‑250 (MQ=255)                              ggATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTa                                                                                                                                                                                                               >  1:160557/1‑250 (MQ=255)                                atatTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACaa                                                                                                                                                                                                            >  1:277883/1‑251 (MQ=255)                                        gTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACAt                                                                                                                                                                                                    >  1:533647/1‑251 (MQ=255)                                            ggCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAA‑‑GTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTcgcg                                                                                                                                                                                                                 <  1:714890/232‑1 (MQ=255)                                              ccGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGa                                                                                                                                                                                              >  1:448115/1‑251 (MQ=255)                                                 gTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAAt                                                                                                                                                                                           >  1:166365/1‑251 (MQ=255)                                                 gTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAAt                                                                                                                                                                                           >  1:827712/1‑251 (MQ=255)                                                                  gTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTcgcg                                                                                                                                                                          >  1:621139/1‑251 (MQ=255)                                                                         ccGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTgg                                                                                                                                                                                                                                         >  1:430231/1‑181 (MQ=255)                                                                               aaGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGgaagaa                                                                                                                                                             <  1:456224/251‑1 (MQ=255)                                                                                    ggTGGATAAACGGCCGATATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGgaag                                                                                                                                                               >  1:233338/1‑244 (MQ=255)                                                                                        gATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGt                                                                                                                                                                                                                                           <  1:118814/164‑1 (MQ=255)                                                                                                gCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAAtt                                                                                                                                                                              >  1:667148/1‑217 (MQ=255)                                                                                                     cATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCaa                                                                                                                                       <  1:342892/251‑1 (MQ=255)                                                                                                     cATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCaa                                                                                                                                       >  1:289549/1‑251 (MQ=255)                                                                                                             caAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTAt                                                                                                                               <  1:238137/251‑1 (MQ=255)                                                                                                                         cGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGGGGCAAATTGCTATCGAACGTCCaa                                                                                                                    >  1:567595/1‑250 (MQ=255)                                                                                                                           tCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAAcc                                                                                                                  >  1:154700/1‑250 (MQ=255)                                                                                                                               gCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAAATGCTATCGAACGTCCAACCCTGGa                                                                                                             >  1:721984/1‑251 (MQ=255)                                                                                                                                cATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGAt                                                                                                            <  1:790029/251‑1 (MQ=255)                                                                                                                                cATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGAt                                                                                                            <  1:21423/251‑1 (MQ=255)                                                                                                                                 aTGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATg                                                                                                           <  1:23000/251‑1 (MQ=255)                                                                                                                                  tgggtgggCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATgg                                                                                                          <  1:441196/251‑1 (MQ=255)                                                                                                                                         gCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAAAGCCGAAGGAAAAGCGGGGCAAATTGCTATCGAACGGCCAAACCTGGATGGCAAGCAg                                                                                                   >  1:450020/1‑251 (MQ=255)                                                                                                                                           atgtgtatagagacagtcactGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGt                                                                                                 <  1:619317/235‑1 (MQ=255)                                                                                                                                            ttGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTg                                                                                                >  1:624415/1‑251 (MQ=255)                                                                                                                                              gATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAggggg                                                                                               >  1:295362/1‑247 (MQ=255)                                                                                                                                              gATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAAAGCCGAAGGAAAAGCGTGGCAAAATGCTATCGAACGTCCAACCCTGGATGGCAAGCAggaggg                                                                                              >  1:98629/1‑247 (MQ=255)                                                                                                                                                  aGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGGGTGGCAAATTGCTATCGAAAGGCCAACCCCGGATGGCAAGCAGGTGGGacaa                                                                                          >  1:801557/1‑251 (MQ=255)                                                                                                                                                   gTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGGAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAAATGCTATCGAACGTACAACCCTGGATGGGAAGCAGGGGGCacaa                                                                                          >  1:835173/1‑250 (MQ=255)                                                                                                                                                   gTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGc                                                                                                                                                          <  1:202130/186‑1 (MQ=255)                                                                                                                                                    ttGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCacaaca                                                                                        >  1:32351/1‑251 (MQ=255)                                                                                                                                                       tcactcacTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGt                                                                                     <  1:295337/251‑1 (MQ=255)                                                                                                                                                       tcactcacTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGt                                                                                     <  1:755156/251‑1 (MQ=255)                                                                                                                                                          ctcactGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAACGGGGCAAAATGCTATCGAACGTCCAACCCTGGATGGCAAGCCGGGGGCAcaacaaagcat                                                                                  >  1:463360/1‑246 (MQ=255)                                                                                                                                                           tcactGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTTGCGCCAAAGGGAAGAAt                                                                                                                                                            >  1:857602/1‑176 (MQ=255)                                                                                                                                                           tcactGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAgg                                                                                                                                                                   <  1:303135/169‑1 (MQ=255)                                                                                                                                                            cactGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGGGGCACAACAAGTCAT‑Cg                                                                               >  1:488330/1‑251 (MQ=255)                                                                                                                                                             actGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑Cgg                                                                              >  1:144498/1‑251 (MQ=255)                                                                                                                                                                gAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAAAGGCGAAGGAAAAGCGGGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGGGGCACAAAAAGTCAT‑CGGttt                                                                           >  1:848758/1‑251 (MQ=255)                                                                                                                                                                     gggCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGGGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCAGAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAAACCTGGGTGGCAAGCAGGGGGCACAAAAAGGCAT‑CGGTTTGaata                                                                      >  1:452635/1‑251 (MQ=255)                                                                                                                                                                          gTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAatat                                                                  <  1:857887/250‑1 (MQ=255)                                                                                                                                                                          gTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAatat                                                                  <  1:413749/250‑1 (MQ=255)                                                                                                                                                                           tAAGTTTTTCCTGGCAAAAGCCGTACCGCAGCTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTGTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAGTCGGCGGTGGAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCGCAACAAGTCAT‑CGGTTTGAATAATatgg                                                                <  1:42386/251‑1 (MQ=255)                                                                                                                                                                               tttttCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATatggcga                                                             <  1:178610/250‑1 (MQ=255)                                                                                                                                                                                       ggCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAAccc                                                                                                                 >  1:281571/1‑191 (MQ=255)                                                                                                                                                                                                         aCTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGaat                                                                       <  1:537628/214‑1 (MQ=255)                                                                                                                                                                                                         aCTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGaat                                                                       <  1:406520/214‑1 (MQ=255)                                                                                                                                                                                                         aCTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAatat                                                                  <  1:468464/219‑1 (MQ=255)                                                                                                                                                                                                          cTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAatta                                 <  1:615421/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGGGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGACGGCGTGCGtt          >  1:200834/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCCAAAGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCCACCCCGGATGGCAAGCAGGGGGCACAACAAGTTAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCg                                       >  1:246003/1‑221 (MQ=255)                                                                                                                                                                                                                                     aaaGGTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGACGGCGTGCGTTTTTc      <  1:476064/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGACGGCGTGCGGTTTTc      >  1:520438/1‑248 (MQ=255)                                                                                                                                                                                                                                        ggTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCCGGATGGCAAGCAGGGGGCACAACAAGTCAT‑CGGTTTGAATAATATGGGGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGAAGGCGTGCGTTTTTccc    >  1:332870/1‑250 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGCAATCGGCGGTGAAATTCGCGCCCAAGGGAAGAATGCCGAAGGAAAAGCGGGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCATCCGGTTT‑AATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAAAAAGACGGCGTGCGTTTTTCCCAt  >  1:241722/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAAt                                                                                                                                                            <  1:706932/98‑1 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGGAAGCAGGGGGCACAACAAGTCAT‑CGGGTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGGAATTATTTTGAACAAGACGGCGGGCGTTTTTCCCAt  >  1:323400/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAAc                         <  1:395560/228‑1 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGGGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTTAAAAAGACGGCGTGCGTTTTTCCCAt  >  1:898827/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGGGGCACAACAAGTCAA‑CGGGTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGAAGGCGTGCGTTTTTCCCAt  >  1:320727/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTTTGGGGTGGATCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAAATCGCGCCAAAGGGCAGAATGCCGAAGGAAAAGGGTGGCCAATTGCCATTGAACGGCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGGGACATCAGGCGATTATCGGAATTATTTTGAACAAGACGGCGGGCGTTTTTCCCAt  >  1:731725/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               CGGTTAAATCAGGTGACGCAGGGTGCCTTGGATATTACAGTCGGGCCGGTAGTGAATCTTTGGGGGTTCGGTCCGGAAAAGCGGGTGGATAAACGGCCGACATCAGCACAAATTGCCGAACGTCAGGCATGGGTGGGCATTGATAAGTTGTCACTCACTGAAGAGGGCGGTAAGTTTTTCCTGGCAAAAGCCGTACCGCAACTTTATGTGGATCTTTCTTCTATCGCCAAAGGTTTTGGGGTGGAGCAAGTGGCGGATTATCTGGAAAGCATTCGCGTACAAAATTACATGGTGGAAATCGGCGGTGAAATTCGCGCCAAAGGGAAGAATGCCGAAGGAAAAGCGTGGCAAATTGCTATCGAACGTCCAACCCTGGATGGCAAGCAGGTGGCACAACAAGTCAT‑CGGTTTGAATAATATGGCGATGGCGACATCAGGCGATTATCGTAATTATTTTGAACAAGACGGCGTGCGTTTTTCCCAT  >  1/744981‑745463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑