Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	721,372	C→T	intergenic (+6104/‑15595)	unknown → / → unknown	tRNA‑Arg‑TCT/tRNA‑Phe‑GAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	721,372	0	C	T	100.0%	186.4 / NA	68	intergenic (+6104/‑15595)	unknown/unknown	tRNA‑Arg‑TCT/tRNA‑Phe‑GAA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/44);  total (24/44)

CGCCTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGACGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTAGAGAC  >  1/721126‑721605                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          cgcCTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGTGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        <  1:444461/250‑1 (MQ=255) cgcCTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        <  1:318185/250‑1 (MQ=255) cgcCTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        <  1:525947/250‑1 (MQ=255) cgcCTTCTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        <  1:884049/250‑1 (MQ=255)   ccTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        <  1:826425/248‑1 (MQ=255)   ccTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAgt                                                                                                                                                                                                                                     >  1:163310/1‑251 (MQ=255)       ttGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTaa                                                                                                                                                                                                                                 <  1:852818/251‑1 (MQ=255)       ttGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTaa                                                                                                                                                                                                                                 >  1:186185/1‑251 (MQ=255)        tGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        >  1:147403/1‑243 (MQ=255)          ccAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATg                                                                                                                                                                                                                              <  1:165130/251‑1 (MQ=255)               aCGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTc                                                                                                                                                                                                                           <  1:538942/249‑1 (MQ=255)                  cTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTc                                                                                                                                                                                                                      <  1:373733/251‑1 (MQ=255)                    gATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGt                                                                                                                                                                                                                       >  1:805705/1‑248 (MQ=255)                       ccGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGc                                                                                                                                                                                                                 <  1:831225/251‑1 (MQ=255)                       ccGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGc                                                                                                                                                                                                                 <  1:749077/251‑1 (MQ=255)                               aGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTAttt                                                                                                                                                                                                            <  1:99558/248‑1 (MQ=255)                                         gAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAg                                                                                                                                                                                                >  1:367961/1‑250 (MQ=255)                                            tAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCg                                                                                                                                                                                              <  1:102349/249‑1 (MQ=255)                                            tAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCg                                                                                                                                                                                              <  1:823200/249‑1 (MQ=255)                                                    tCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCg                                                                                                                                                                                              >  1:770901/1‑241 (MQ=255)                                                     cAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGa                                                                                                                                                                                   >  1:144768/1‑251 (MQ=255)                                                       gTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCg                                                                                                                                                                                              >  1:785986/1‑238 (MQ=255)                                                           gCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTAt                                                                                                                                                                              >  1:771807/1‑250 (MQ=255)                                                                           gATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAGTTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAAt                                                                                                                                                             >  1:466837/1‑251 (MQ=255)                                                                           gATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATg                                                                                                                                                                                                                              <  1:412794/186‑1 (MQ=255)                                                                           gATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAAt                                                                                                                                                             >  1:547332/1‑251 (MQ=255)                                                                            aTTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGaa                                                                                                                                                              <  1:583815/249‑1 (MQ=255)                                                                                      tAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTACAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGc                                                                                                                                                   <  1:38998/250‑1 (MQ=255)                                                                                        gCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCg                                                                                                                                                                                              <  1:531231/205‑1 (MQ=255)                                                                                             aaaCCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGc                                                                                                                                                                                                                 <  1:707663/181‑1 (MQ=255)                                                                                                   cgTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATaaaaa                                                                                                                                      <  1:7518/250‑1 (MQ=255)                                                                                                                                                  cgcTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGt                                                                                                                                                                                                                                        <  1:628604/105‑1 (MQ=255)                                                                                                                                                  cgcTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCttt                                                                                      >  1:851812/1‑251 (MQ=255)                                                                                                                                                      tactCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGAc                                                                                  <  1:3177/248‑1 (MQ=255)                                                                                                                                                      tATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTc                                                                                                                                                                                                                      <  1:837471/119‑1 (MQ=255)                                                                                                                                                      tATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGa                                                                                   >  1:87799/1‑250 (MQ=255)                                                                                                                                                      tATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGAc                                                                                  <  1:720752/251‑1 (MQ=255)                                                                                                                                                      tATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGAc                                                                                  <  1:513053/251‑1 (MQ=255)                                                                                                                                                       aTTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACa                                                                                 >  1:901004/1‑251 (MQ=255)                                                                                                                                                           tAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGc                                                                             <  1:128581/251‑1 (MQ=255)                                                                                                                                                                 aTTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGc                                                                          <  1:354548/248‑1 (MQ=255)                                                                                                                                                                 aTTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCa                                                                        >  1:29049/1‑250 (MQ=255)                                                                                                                                                                           gCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGCGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGc                                                                                         >  1:236627/1‑223 (MQ=255)                                                                                                                                                                              cTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGaaagtggaaaccgac                                                                                                                                          <  1:95178/171‑13 (MQ=255)                                                                                                                                                                                     ggATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGttt                                                   >  1:143279/1‑251 (MQ=255)                                                                                                                                                                                      gATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGttt                                                   <  1:789001/250‑1 (MQ=255)                                                                                                                                                                                       aTTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACAt                                                                                >  1:632071/1‑220 (MQ=255)                                                                                                                                                                                          tttGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACt                                              <  1:215667/251‑1 (MQ=255)                                                                                                                                                                                                  ggATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGgag                                                                                                                                              <  1:658814/147‑1 (MQ=255)                                                                                                                                                                                                  ggATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGAc                                                                                  <  1:870072/207‑1 (MQ=255)                                                                                                                                                                                                  ggATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGAt                                      >  1:678078/1‑251 (MQ=255)                                                                                                                                                                                                          gAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAAc                                                                                                                                                                                 <  1:85937/104‑1 (MQ=255)                                                                                                                                                                                                               aaaGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCa                           >  1:24564/1‑249 (MQ=255)                                                                                                                                                                                                                 aGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGT‑AATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACtt                        >  1:20831/1‑249 (MQ=255)                                                                                                                                                                                                                      aTGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCTCTTTCTCGATGCCTTCGCCCACTTCTAAAc                  <  1:622243/251‑1 (MQ=255)                                                                                                                                                                                                                      aTGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAAc                  <  1:477999/251‑1 (MQ=255)                                                                                                                                                                                                                      aTGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAAc                  <  1:290868/251‑1 (MQ=255)                                                                                                                                                                                                                      aTGGGTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAAc                  <  1:420367/251‑1 (MQ=255)                                                                                                                                                                                                                        gggTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACgg                >  1:452165/1‑251 (MQ=255)                                                                                                                                                                                                                         ggTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAAt                                                                                                                                                             <  1:732384/109‑1 (MQ=255)                                                                                                                                                                                                                         ggTTATAACGGAAATGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATAcc                                   <  1:841794/231‑1 (MQ=255)                                                                                                                                                                                                                                    aaaTGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTagag    <  1:11357/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTAGAGAc  <  1:652202/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTAGAGAc  <  1:644739/251‑1 (MQ=255)                                                                                                                                                                                                                                       tGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCAc                                               <  1:115382/205‑1 (MQ=255)                                                                                                                                                                                                                                       tGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTagag    >  1:663110/1‑248 (MQ=255)                                                                                                                                                                                                                                       tGTCGGCACTGATTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTAgaga   >  1:246960/1‑249 (MQ=255)                                                                                                                                                                                                                                                  aTTGATGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCtt                                 <  1:808390/208‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CGCCTTTTGCCAAGACGCTGATCCGCCCGAAGTCTTCCGTGAGTAAATCGATCAGTAAGCTGGTTTCGCTGTAAGATTTGCGATGTAGCACGAAACCGCGTTGCCAATTTTCGATGATGTCGCCCTTTTCGCCGATAAAAAATTGCGCTTATTCTAGCAGATTTCACACCGCACTTTGTGGGATTTTTGCGTGGGATTTTAGAATAAAAGGGAATGGGTTATAACGGAAATGTCGGCACTGATTGACGGTAGTGTAAATGTTCAGGTCTGTGCTATTTTATCGGTCTAAGCGTGGACGCTTGAACTATCGGGCAAGCGTGAGAATCGAGAAATGCCGGAGAATAAAAAAGCAGGTTTTGACACCTGCTTTTTTGCTTAAATCATCAATTAAGCTTTAGACATTGCAGCCACTTCTGCGGCAAAGTCGGTTTCCACTTTCTCGATACCTTCGCCCACTTCTAAACGGATGAAGTTAGAGAC  >  1/721126‑721605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑