Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	716,463	T→C	intergenic (+1195/‑20504)	unknown → / → unknown	tRNA‑Arg‑TCT/tRNA‑Phe‑GAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	716,463	0	T	C	100.0%	100.8 / NA	37	intergenic (+1195/‑20504)	unknown/unknown	tRNA‑Arg‑TCT/tRNA‑Phe‑GAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/14);  total (23/14)

CACTATTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAGCGGAGCCTTGATCTTTGGCACGCCCGGAGAGTTCGTGGCGTTCGGTGCCGATGCG  >  1/716328‑716706                                                                                                                                        |                                                                                                                                                                                                                                                    cACTATTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCAttttt                                                                                                                                  >  1:242576/1‑251 (MQ=17)     aTTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCg                                                                                                                              >  1:807172/1‑251 (MQ=21)               gTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAATTCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGGTAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTa                                                                                                                    >  1:608973/1‑251 (MQ=255)                    gggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTaa                                                                                                               >  1:880833/1‑251 (MQ=255)                     ggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTaaa                                                                                                              >  1:357001/1‑251 (MQ=255)                        ttATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAaacaa                                                                                                           <  1:168261/251‑1 (MQ=255)                          atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGt                                                                                                                  >  1:187441/1‑242 (MQ=255)                          atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAacaaca                                                                                                         >  1:301352/1‑251 (MQ=255)                          atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAacaaca                                                                                                         >  1:379174/1‑251 (MQ=255)                          atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAacaaca                                                                                                         >  1:487212/1‑251 (MQ=255)                            ataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACAtt                                                                                                       <  1:534749/251‑1 (MQ=255)                                ggggATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAAt                                                                                                    >  1:472858/1‑250 (MQ=255)                                 gggATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATc                                                                                                   <  1:896022/250‑1 (MQ=255)                                 gggATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATc                                                                                                   <  1:50358/250‑1 (MQ=255)                                  ggATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATcc                                                                                                  <  1:785495/250‑1 (MQ=255)                                          cGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGAc                                                                                         <  1:480213/251‑1 (MQ=255)                                            ccATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCa                                                                                       >  1:193772/1‑251 (MQ=255)                                                   gCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGAt                                                                                >  1:485290/1‑251 (MQ=255)                                                     ccTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATaa                                                                              <  1:305195/251‑1 (MQ=255)                                                       tctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACt                                                                            <  1:766715/251‑1 (MQ=255)                                                        ctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAGATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAAc                                                                             >  1:877314/1‑249 (MQ=255)                                                        ctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTa                                                                           >  1:509496/1‑251 (MQ=255)                                                         tttCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTaa                                                                          <  1:524790/251‑1 (MQ=255)                                                                          ccAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATc                                                                                                   <  1:737416/209‑1 (MQ=255)                                                                          ccAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAg                                                         >  1:415799/1‑251 (MQ=255)                                                                                             cGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAaaca                                                                                                            <  1:622019/181‑1 (MQ=255)                                                                                                         tgaAATTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAGCGGAGCCTTGATCTTTGGCACGCCCGGagag                          <  1:656662/251‑1 (MQ=255)                                                                                                            aaTTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGa                                                                                 >  1:455492/1‑193 (MQ=255)                                                                                                            aaTTTAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAGCGGAGCCTTGATCTTTGGCACGCCCGGAGAGtt                        >  1:6153/1‑250 (MQ=255)                                                                                                             gtttcgCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGttttt                                                                                                                      >  1:275424/6‑155 (MQ=255)                                                                                                             gtttcgCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGc                                                           >  1:668595/6‑214 (MQ=255)                                                                                                             gtttcgCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGc                                                           <  1:770060/209‑1 (MQ=255)                                                                                                             gtttcgCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGc                                                           >  1:45632/6‑214 (MQ=255)                                                                                                                     aCCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAaacaac                                                                                                          >  1:739342/1‑159 (MQ=255)                                                                                                                      ccTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATc                                                                                                   >  1:716176/1‑165 (MQ=255)                                                                                                                               tACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAGCGGAGCCTTGATCTTTGGCACGCCCGGAGAGTTCGTGGCGTTCGGTGCCGATg    <  1:471524/251‑1 (MQ=255)                                                                                                                                 ccAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAGCGGAGCCTTGATCTTTGGCACGCCCGGAGAGTTCGTGGCGTTCGGTGCCGATGCg  >  1:12202/1‑251 (MQ=255)                                                                                                                                        |                                                                                                                                                                                                                                                    CACTATTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTCATTTTTGCCGACGTTTTTTAGTTAAACAACATTAATCCCCAAGTGACCACTACGATAACTAAGGCGACAAACACGGCAGCGGAGCCTTGATCTTTGGCACGCCCGGAGAGTTCGTGGCGTTCGGTGCCGATGCG  >  1/716328‑716706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑