| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 5,217 | C→T | intergenic (–/+125917) | – / ← unknown | –/tRNA‑Ala‑GGC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 5,217 | 0 | C | T | 93.3% | 65.4 / ‑2.9 | 30 | intergenic (–/+125917) | –/unknown | –/tRNA‑Ala‑GGC |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (18/10); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01 | |||||||||||
CCGGTTTTAGCCCCACGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCCGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCACGCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAG > 1/4975‑5438 | ccGGTTTTAGCCCCACGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc < 1:493246/251‑1 (MQ=255) cGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAgg > 1:735912/1‑251 (MQ=255) cATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGAt > 1:732022/1‑251 (MQ=255) aTTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGGCACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAg > 1:645318/1‑235 (MQ=255) cacTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTa > 1:790635/1‑251 (MQ=255) cTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTAt > 1:11409/1‑250 (MQ=255) cTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTAt > 1:237662/1‑250 (MQ=255) cTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTAt > 1:93824/1‑250 (MQ=255) gTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTa < 1:504748/239‑1 (MQ=255) cgGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGctgaa > 1:635018/1‑247 (MQ=255) cgGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGctgaa > 1:631828/1‑247 (MQ=255) gTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGctgaac < 1:475827/250‑6 (MQ=255) cTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCCAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAt > 1:514412/1‑251 (MQ=255) cccTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAtca > 1:555863/1‑249 (MQ=255) ccTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAtcag < 1:134948/251‑4 (MQ=255) cTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCTGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGt < 1:434363/234‑1 (MQ=255) cTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGt > 1:592600/1‑234 (MQ=255) gATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTg > 1:591187/1‑225 (MQ=255) aTCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAtcagatttct > 1:846594/1‑241 (MQ=21) tCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAtcagatttctcg < 1:443709/251‑12 (MQ=21) gtggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAtcagatttctcgttctcaag < 1:105708/250‑20 (MQ=18) ggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc > 1:792451/1‑182 (MQ=255) ggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCAtc < 1:737475/230‑2 (MQ=255) tAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAgg > 1:156129/1‑210 (MQ=255) cggcggcggTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGctgaact < 1:518583/190‑7 (MQ=255) gcggcggTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAAt < 1:675978/250‑1 (MQ=11) gtAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAg > 1:524268/1‑121 (MQ=255) aaaTAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGt > 1:666057/1‑136 (MQ=255) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg > 1:897128/1‑251 (MQ=18) ctTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAAtc > 1:94868/1‑83 (MQ=25) | CCGGTTTTAGCCCCACGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCCGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCACGCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAG > 1/4975‑5438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |