Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	677,805	T→C	intergenic (+180252/‑21538)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	677,805	0	T	C	100.0%	173.1 / NA	62	intergenic (+180252/‑21538)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/20);  total (42/20)

GTACATATTCAGGTGTGGGGGCTTGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTGGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGTCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGACGATGCGCCG  >  1/677559‑678034                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      gTACATATTCAGGTGTGGGGGCTTGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAAt                                                                                                                                                                                                                                   >  1:793545/1‑251 (MQ=255)            ggTGTGGGGGCTTGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACgc                                                                                                                                                                                                                         >  1:460267/1‑250 (MQ=255)                      cTTGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACgccgaatgccgaa                                                                                                                                                                                                              <  1:842373/251‑1 (MQ=255)                        tGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGccgaatgccgaatg                                                                                                                                                                                                            >  1:442788/1‑251 (MQ=255)                        tGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGccgaatgccgaatg                                                                                                                                                                                                            >  1:308384/1‑251 (MQ=255)                        tGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGccgaatgccgaatg                                                                                                                                                                                                            >  1:196848/1‑251 (MQ=255)                             gTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCg                                                                                                                                                                                                       >  1:556972/1‑251 (MQ=255)                               aCATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGtt                                                                                                                                                                                                     >  1:240774/1‑251 (MQ=255)                               aCATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCATGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGtt                                                                                                                                                                                                     >  1:179338/1‑251 (MQ=255)                                         cccTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATc                                                                                                                                                                                                                                  >  1:234697/1‑212 (MQ=255)                                          ccTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGcc                                                                                                                                                                                           >  1:748637/1‑250 (MQ=255)                                                  gaaaagGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCAc                                                                                                                                                                                        >  1:144035/1‑245 (MQ=255)                                                                              gTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCgacgac                                                                                                                                                                 >  1:276236/1‑240 (MQ=255)                                                                                      tAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGAc                                                                                                                                               <  1:556632/250‑1 (MQ=255)                                                                                           aaaaaaCGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGttt                                                                                                                                          >  1:715497/1‑250 (MQ=255)                                                                                             aaaaCGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTtctc                                                                                                                                       <  1:790803/251‑1 (MQ=255)                                                                                                         ggCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGa                                                                                                                           >  1:134867/1‑251 (MQ=255)                                                                                                          gCGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAAtgt                                                                                                                                                                                                           >  1:168526/1‑170 (MQ=255)                                                                                                           cGTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACg                                                                                                                         <  1:5407/251‑1 (MQ=255)                                                                                                            gTATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAAtgt                                                                                                                                                                                                           <  1:397423/168‑1 (MQ=255)                                                                                                             tATCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGc                                                                                                                                                                                            <  1:583049/182‑1 (MQ=255)                                                                                                              aTCATATCCGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGAc                                                                                                                                               >  1:167884/1‑226 (MQ=255)                                                                                                                     ccGACAAATTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGc                                                                                                                 >  1:705569/1‑249 (MQ=255)                                                                                                                           aaTTTAATCTGATTTTATTGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGtt                                                                                                         >  1:858716/1‑251 (MQ=255)                                                                                                                                            ttGTAGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGaaa                                                                                        >  1:241811/1‑251 (MQ=255)                                                                                                                                             tgtaggAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAAt                                                                                       <  1:504657/247‑1 (MQ=255)                                                                                                                                              gtaggAGCGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCCCTCATATTCGTATTTTCGTAGCCTATCCCGGCACGCCGGATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCcgac                                                                                                                                                                    <  1:237137/170‑1 (MQ=255)                                                                                                                                              gtaggAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCTGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAAtt                                                                                      >  1:630359/4‑251 (MQ=255)                                                                                                                                              gtaggAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTtctc                                                                                                                                       >  1:413847/4‑202 (MQ=255)                                                                                                                                              gtaggAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGcc                                                                                                                                   >  1:13429/4‑206 (MQ=255)                                                                                                                                               taggAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGc                                                                                                                                                                                            >  1:375576/3‑148 (MQ=255)                                                                                                                                                  gagaGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGAt                                                                                   <  1:420740/250‑1 (MQ=255)                                                                                                                                                    gagTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTa                                                                                 >  1:113480/1‑250 (MQ=255)                                                                                                                                                              acaaATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCGAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCgaag                                                                      >  1:436342/1‑251 (MQ=255)                                                                                                                                                                 aaTTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATAc                                                                                                                                                  <  1:351937/172‑1 (MQ=255)                                                                                                                                                                        aTTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGgcg                                                             >  1:859166/1‑250 (MQ=255)                                                                                                                                                                         tttCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGaaa                                                                                                                      >  1:353950/1‑192 (MQ=255)                                                                                                                                                                         tttCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCAc                                                                           <  1:643042/235‑1 (MQ=255)                                                                                                                                                                          ttCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCga                                                                        >  1:208551/1‑237 (MQ=255)                                                                                                                                                                                    gcgcAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGcc                                                                                                                <  1:876136/187‑1 (MQ=255)                                                                                                                                                                                     cgcAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCtt                                                <  1:899883/250‑1 (MQ=255)                                                                                                                                                                                           cgcATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAg                                           <  1:363194/249‑1 (MQ=255)                                                                                                                                                                                           cgcATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAg                                           <  1:263914/249‑1 (MQ=255)                                                                                                                                                                                                       gTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTa                                               <  1:362414/233‑1 (MQ=255)                                                                                                                                                                                                       gTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAg                                           <  1:97025/237‑1 (MQ=255)                                                                                                                                                                                                       gTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCaaa                             >  1:561098/1‑251 (MQ=255)                                                                                                                                                                                                       gTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCaaa                             >  1:145596/1‑251 (MQ=255)                                                                                                                                                                                                        tttATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCaaaa                            <  1:704122/251‑1 (MQ=255)                                                                                                                                                                                                        tttATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCaaaa                            <  1:74725/251‑1 (MQ=255)                                                                                                                                                                                                         ttATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCga                                                                        <  1:710597/206‑1 (MQ=255)                                                                                                                                                                                                               gATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGaaa                     >  1:463958/1‑251 (MQ=255)                                                                                                                                                                                                                  cAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCtt                                                >  1:729778/1‑221 (MQ=255)                                                                                                                                                                                                                  cAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGt                  >  1:633110/1‑251 (MQ=255)                                                                                                                                                                                                                       ggAAGAAGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCgg             >  1:213257/1‑251 (MQ=255)                                                                                                                                                                                                                        gaagaaGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGa            >  1:97053/1‑251 (MQ=255)                                                                                                                                                                                                                        gaagaaGGCACTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGa            >  1:558436/1‑251 (MQ=255)                                                                                                                                                                                                                                  cTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGACGATGcgccg  >  1:85301/1‑251 (MQ=255)                                                                                                                                                                                                                                  cTCATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGACGATGcgccg  >  1:893059/1‑251 (MQ=255)                                                                                                                                                                                                                                    cATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACg                                                                               >  1:242596/1‑172 (MQ=255)                                                                                                                                                                                                                                    cATATTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGACGATGcgccg  >  1:460191/1‑249 (MQ=255)                                                                                                                                                                                                                                     atatTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAg                                           >  1:225126/1‑207 (MQ=255)                                                                                                                                                                                                                                      tatTCGTATTTTCGTAGCCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAg                                                                                                                                                                         >  1:406722/1‑80 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      GTACATATTCAGGTGTGGGGGCTTGCCCGTACATAACGCTCCCTTAAAAGAAAAGGCTAATCCGCTTTTTTATTATAGTAAAAGGTAGAAAAAAAACGGCGTGAGGCGTATCATATCCGACAAATTTAATCTGATTTTATTGTGGGAGAGTTATGCAACAAATTACTATTTCAGAAGCGGCGCAAGCGCATTTTCGCCGTTTATTGGATCAACAGGAAGAAGGCACTCATATTCGTATTTTCGTAGTCAATCCCGGCACGCCGAATGCCGAATGTGGCGTTTCTTACTGCCCACCGAATTCCGTTGAAGCCGACGACATGGAAATCAAATACGACAGTTTCTCCGCCTTTGTAGACGAAATCAGCCTGCCGTTTCTGGAAGATGCGGAAATTGATTACGTCACCGAAGAACTGGGCGCGCAGCTCACCTTAAAAGCGCCGAATGCCAAAATGAGAAAAGTCGCGGACGATGCGCCG  >  1/677559‑678034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑