Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	666,141	C→T	intergenic (+168588/‑33202)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	666,141	0	C	T	100.0%	218.5 / NA	81	intergenic (+168588/‑33202)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/47);  total (34/47)

TAATACGGCTATTTCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCCTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCTGCCGTT  >  1/665894‑666383                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   taataCGGCTATTTCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTg                                                                                                                                                                                                                                                 >  1:531965/1‑251 (MQ=255)           aTTTCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGa                                                                                                                                                                                                                                        >  1:869856/1‑250 (MQ=255)              tCCTTTGGCAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGa                                                                                                                                                                                                                                        >  1:226621/1‑247 (MQ=255)              tCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGa                                                                                                                                                                                                                                        >  1:80810/1‑247 (MQ=255)              tCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGa                                                                                                                                                                                                                                        >  1:869355/1‑247 (MQ=255)              tCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGa                                                                                                                                                                                                                                        >  1:611094/1‑247 (MQ=255)              tCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTg                                                                                                                                                                                                                                     >  1:641437/1‑250 (MQ=255)               ccTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTa                                                                                                                                                                                                                                   >  1:23483/1‑251 (MQ=255)               ccTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACATACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTa                                                                                                                                                                                                                                   >  1:447659/1‑251 (MQ=255)                      cAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGcc                                                                                                                                                                                                                                               <  1:896641/232‑1 (MQ=255)                        gTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATc                                                                                                                                                                                                                             <  1:659004/248‑1 (MQ=255)                         tGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATc                                                                                                                                                                                                                             <  1:21538/247‑1 (MQ=255)                         tGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATc                                                                                                                                                                                                                             <  1:16667/247‑1 (MQ=255)                           aaaaaTAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCgccgcc                                                                                                                                                                                                                       <  1:874644/251‑1 (MQ=255)                                taaaataCGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGc                                                                                                                                                                                                                  <  1:190783/251‑1 (MQ=255)                                  aaataCGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGccc                                                                                                                                                                                                                <  1:285008/251‑1 (MQ=255)                                  aaataCGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGccc                                                                                                                                                                                                                <  1:693282/251‑1 (MQ=255)                                  aaataCGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGccc                                                                                                                                                                                                                <  1:870969/251‑1 (MQ=255)                                     taCGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCcg                                                                                                                                                                                                              >  1:610318/1‑250 (MQ=255)                                            acaATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATAc                                                                                                                                                                                                      <  1:201324/251‑1 (MQ=255)                                             caATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATAc                                                                                                                                                                                                      >  1:825722/1‑250 (MQ=255)                                               aTTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCAc                                                                                                                                                                                                   <  1:46919/251‑1 (MQ=255)                                                 tGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCGTGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATcgc                                                                                                                                                                                                                           >  1:12842/1‑225 (MQ=255)                                                 tGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACtc                                                                                                                                                                                                 <  1:200484/251‑1 (MQ=255)                                                  gCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACt                                                                                                                                                                                                                                      <  1:84896/213‑1 (MQ=255)                                                   ccTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTgttggaggtgag                                                                                                                                                                                               <  1:624537/251‑12 (MQ=255)                                                      ttttCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCAc                                                                                                                                                                                            <  1:818795/251‑1 (MQ=255)                                                      ttttCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCAc                                                                                                                                                                                            <  1:205018/251‑1 (MQ=255)                                                         tctttcACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTAt                                                                                                                                                                                         <  1:610869/251‑1 (MQ=255)                                                          ctttcACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAAT‑TAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGa                                                                                                                                                                                       <  1:812321/251‑1 (MQ=255)                                                                            aaaTAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCgccgcc                                                                                                                                                                                                                       <  1:154571/202‑1 (MQ=255)                                                                            aaaTAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGa                                                                                                                                                                      <  1:863148/251‑1 (MQ=255)                                                                               tAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCaa                                                                                                                                                                                <  1:631153/238‑1 (MQ=255)                                                                                   aaaGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTcccc                                                                                                                                                               <  1:631982/251‑1 (MQ=255)                                                                                   aaaGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTcccc                                                                                                                                                               <  1:613725/251‑1 (MQ=255)                                                                                      gATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTAt                                                                                                                                                                                         <  1:680386/222‑1 (MQ=255)                                                                                      gATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGa                                                                                                                                                            >  1:115297/1‑251 (MQ=255)                                                                                        tAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAg                                                                                                                                                          <  1:399503/251‑1 (MQ=255)                                                                                        tAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAg                                                                                                                                                          <  1:713074/251‑1 (MQ=255)                                                                                        tAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAg                                                                                                                                                          <  1:506718/251‑1 (MQ=255)                                                                                                      aTTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTc                                                                                                                                            <  1:14074/251‑1 (MQ=255)                                                                                                          tACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAAcc                                                                                                                                        <  1:502420/251‑1 (MQ=255)                                                                                                          tACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAAcc                                                                                                                                        <  1:376871/251‑1 (MQ=255)                                                                                                                ggCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTc                                                                                                                                                                                  >  1:717937/1‑203 (MQ=255)                                                                                                                       gTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTaaaa                                                                                                                             >  1:236159/1‑249 (MQ=255)                                                                                                                             aaGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACtt                                                                                                                     <  1:749137/251‑1 (MQ=255)                                                                                                                               gCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGGAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAg                                                                                                                                                                              <  1:34880/192‑1 (MQ=255)                                                                                                                                cAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGa                                                                                                                                                                      <  1:468151/199‑1 (MQ=255)                                                                                                                                           ccGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACtcatc                                                                                                                                                                                              >  1:340976/1‑164 (MQ=255)                                                                                                                                           ccGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTAt                                                                                                                                                                                         <  1:200315/169‑1 (MQ=255)                                                                                                                                           ccGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTAt                                                                                                                                                                                         >  1:858542/1‑169 (MQ=255)                                                                                                                                           ccGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAAcc                                                                                                            >  1:662624/1‑246 (MQ=255)                                                                                                                                           ccGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCa                                                                                                        >  1:620796/1‑250 (MQ=255)                                                                                                                                                     cATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAgt                                                                                             <  1:400392/251‑1 (MQ=255)                                                                                                                                                      aTGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGa                                                                                                                                                                                       >  1:247063/1‑160 (MQ=255)                                                                                                                                                        gTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGccc                                                                                                                                                                                                                >  1:724266/1‑133 (MQ=255)                                                                                                                                                        gTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAAt                                                                                                                                                                    >  1:58617/1‑177 (MQ=255)                                                                                                                                                           ttCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTc                                                                                                                                            <  1:27695/198‑1 (MQ=255)                                                                                                                                                               ttATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGAc                                                                                   <  1:468043/251‑1 (MQ=255)                                                                                                                                                                      tAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGc                                                                                                                                                         >  1:873888/1‑174 (MQ=255)                                                                                                                                                                      tAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTg                                                                            <  1:335577/251‑1 (MQ=255)                                                                                                                                                                           aTGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGcc                                                                                                                                                                                                                                               >  1:238798/1‑83 (MQ=255)                                                                                                                                                                           aTGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAg                                                                                                                                                                              <  1:187310/148‑1 (MQ=255)                                                                                                                                                                           aTGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAAt                                                                                                                                                                    <  1:891574/158‑1 (MQ=255)                                                                                                                                                                           aTGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCg                                                                                                                                      <  1:446063/188‑1 (MQ=255)                                                                                                                                                                                   cTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGat                                                                                                                                                                                      >  1:580376/1‑132 (MQ=255)                                                                                                                                                                                             gTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGa                                                                                                                                                                                       >  1:432713/1‑121 (MQ=255)                                                                                                                                                                                                            aCTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGt                                                                                                                                                                                                           <  1:81714/86‑1 (MQ=255)                                                                                                                                                                                                               aTCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCaa                                                                                                                                                                                >  1:532708/1‑110 (MQ=255)                                                                                                                                                                                                               aTCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAAcc                                                                                                                                        <  1:627820/150‑1 (MQ=255)                                                                                                                                                                                                               aTCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTAGGTAAAcc                                                                                                            >  1:591238/1‑178 (MQ=255)                                                                                                                                                                                                                          aTTTTCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAg                                                                                              <  1:304744/181‑1 (MQ=255)                                                                                                                                                                                                                              tCATGAAACTGATGACTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGc                                                                                                                                                                                                                  >  1:42836/1‑61 (MQ=255)                                                                                                                                                                                                                                        gatgaCTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGt          >  1:11625/1‑251 (MQ=255)                                                                                                                                                                                                                                          tgaCTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCt        <  1:209143/251‑1 (MQ=255)                                                                                                                                                                                                                                            aCTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCTGc      <  1:1525/251‑1 (MQ=255)                                                                                                                                                                                                                                             cTAAATTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCTGc      >  1:147398/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaaTTAACCTTTGCCGTTGCGACTGTATTTATcgc                                                                                                                                                                                                                           >  1:331966/1‑35 (MQ=255)                                                                                                                                                                                                                                               aaaTTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAg                                                                                                                                                          <  1:143606/100‑1 (MQ=255)                                                                                                                                                                                                                                               aaaTTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCTGCCGt   >  1:830734/1‑251 (MQ=255)                                                                                                                                                                                                                                                aaTTAACCTTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCTGCCGtt  >  1:301287/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   TAATACGGCTATTTCCTTTGACAGTGAAAAATAAAATACGATCACAATTGCCTTTTTCTTTCACCGGTTCGGCTAAAATAGTAAAGATAAAAACCGGGTCAATTTTACCTAGGCTCCGGTTCGAAAGCAATTGGCGCACCGCAAGAACCATGTATTCGTTATCAATAAAAATGCTATACTTACGTACGGTTTTCATCTTTTTTACTATCAAAAGGAGATTTTCATGAAACTGATGACTAAATTAACCCTTGCCGTTGCGACTGTATTTATCGCCGCCTGTGCCCCGCGTGATACCACTCATCACTATGATATTCAAAGTGTATTGAATTCCCCTGAAGCACAGCGTTTCCTCAACCCGAATGTAAAACTTTACTTCGGTAAACCGGCACCGGGTAAAGTGGTGGTCGACAATGCTGTCACCAACAAGAAAACCAATGCTGCCAACAAATCTGACGAAAAAGCCTGCAAACGCGCTTTCTTGTCTGCCGTT  >  1/665894‑666383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑