Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	599,492	G→T	intergenic (+101939/‑99851)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	599,492	0	G	T	100.0%	27.9 / NA	12	intergenic (+101939/‑99851)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/5);  total (7/5)

GATTAACTATCCGTAGAAGATCAGGCCAAATCTGACAAACCACAATAAAAAGTGAGAATTTCCCGTTTAGAAACAGCCGCAACTATTTTTACTGGAGAACTACCAAAAACTAAAGATAAATTATCAATTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTGATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTATTGATGCCAAATTAATTCGACGACTGTTGGCTGAAACCACAAAAGCAAACATCGCAAATGCGGATAATAAAGTAGCTAATAACACAATGAAACCCCTA  >  1/599245‑599731                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                gaTTAACTATCCGTAGAAGATCAGGCCAAATCTGACAAACCACAATAAAAAGTGAGAATTTCCCGTTTAGAAACAGCCGCAACTATTTTTACTGGAGAACTACCAAAAACTAAAGATAAATTATCAATTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTtata                                                                                                                                                                                                                                              >  1:315872/1‑251 (MQ=255)      aCTATCCGTAGAAGATCAGGCCAAATCTGACAAACCACAATAAAAAGTGAGAATTTCCCGTTTAGAAACAGCCGCAACTATTTTTACTGGAGAACTACCAAAAACTAAAGATAAATTATCAATTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAAtt                                                                                                                                                                                                                                         >  1:453281/1‑251 (MQ=255)             gTAGAAGATCAGGCCAAATCTGACAAACCACAATAAAAAGTGAGAATTTCCCGTTTAGAAACAGCCGCAACTATTTTTACTGGAGAACTACCAAAAACTAAAGATAAATTATCAATTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCta                                                                                                                                                                                                                                  >  1:154019/1‑251 (MQ=255)                                                                                     aTTTTTACTGGAGAACTACCAAAAACTAAAGATAAATTATCAATTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAg                                                                                                                                                                                                                                             <  1:603052/168‑1 (MQ=255)                                                                                                                               aTTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAat                                                                                                                                                                                                                            <  1:577694/143‑1 (MQ=255)                                                                                                                                                                          cTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTATATTTAATAATCGTCTATATTc                                                                                                                                >  1:450970/1‑192 (MQ=255)                                                                                                                                                                           tatttattCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTc                                                                                                                                >  1:668521/1‑191 (MQ=255)                                                                                                                                                                                                  tataAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGat                                                                                                                     <  1:867905/179‑1 (MQ=255)                                                                                                                                                                                                            gTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTAt                                                                                                   >  1:900704/1‑187 (MQ=255)                                                                                                                                                                                                               ttCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTATTGATGCCAAATTAATTCGACGACTGTTGGCTGAAACCACAAAAGCAAACATCGCaaa                                          >  1:361626/1‑241 (MQ=255)                                                                                                                                                                                                                                aaTATTTGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTATTGATGCCAAATTAATTCGACGACTGTTGGCTGAAACCACAAAAGCAAACATCGCAAATGCGGATAATAAAGTAGCTAATAacac               <  1:540564/251‑1 (MQ=255)                                                                                                                                                                                                                                      tGCAAGTCAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTATTGATGCCAAATTAATTCGACGACTGTTGGCTGAAACCACAAAAGCAAACATCGCAAATGCGGATAATAAAGTAGCTAATAACACAATGaa         <  1:868913/251‑1 (MQ=255)                                                                                                                                                                                                                                             cAAATATATCTTATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTATTGATGCCAAATTAATTCGACGACTGTTGGCTGAAACCACAAAAGCAAACATCGCAAATGCGGATAATAAAGTAGCTAATAACACAATGAAACCCCTa  >  1:891153/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GATTAACTATCCGTAGAAGATCAGGCCAAATCTGACAAACCACAATAAAAAGTGAGAATTTCCCGTTTAGAAACAGCCGCAACTATTTTTACTGGAGAACTACCAAAAACTAAAGATAAATTATCAATTTCGCTCTCTCTCATTTTTTCCTCGTATTTTACTAAATAAACTATTTATTCTTTCTTTAAATGAATATAAAACCAGTATTCCCAAATAGATATAAAATATTTGCAAGTCAAATATATCTGATAGAATTCTTGCTATAAAATATATTAACAGGCAAAAAAGAGTAAATTGTATGAAATTTTTCACTTGTTTTCCTCAATATAAGTAATATTGTATTTAATAATCGTCTATATTCTAATTCAAGATATTCCATCTGAGCTTTATTGATGCCAAATTAATTCGACGACTGTTGGCTGAAACCACAAAAGCAAACATCGCAAATGCGGATAATAAAGTAGCTAATAACACAATGAAACCCCTA  >  1/599245‑599731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑