Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	593,451	C→A	intergenic (+95898/‑105892)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	593,451	0	C	A	95.2%	204.7 / 1.5	84	intergenic (+95898/‑105892)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (49/31);  total (50/34)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

GTGTTACACCGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGCTCTGCACCCCAAAACCT‑‑‑‑‑‑GGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTG  >  1/593210‑593683                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               gtgtTACACCGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCAcc                                                                                                                                                                                                                                        >  1:508477/1‑250 (MQ=255) gtgtTACACCGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCAcc                                                                                                                                                                                                                                        >  1:120071/1‑250 (MQ=255) gtgtTACACCGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCAcc                                                                                                                                                                                                                                        >  1:91608/1‑250 (MQ=255)       cacCGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAAc                                                                                                                                                                                                                                 <  1:350757/251‑1 (MQ=255)         ccGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAAc                                                                                                                                                                                                                                 >  1:776019/1‑249 (MQ=255)           gTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTgg                                                                                                                                                                                                                             >  1:329248‑M1/1‑249 (MQ=255)                   gATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacgga                                                                                                                                                                                                                      >  1:765984‑M1/1‑241 (MQ=255)                    aTCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggaca                                                                                                                                                                                                                    >  1:109894‑M1/1‑240 (MQ=255)                    aTCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggaca                                                                                                                                                                                                                    >  1:477500‑M1/1‑240 (MQ=255)                    aTCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggaca                                                                                                                                                                                                                    >  1:371620‑M1/1‑240 (MQ=255)                           cAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatt                                                                                                                                                                                                             <  1:747661‑M1/251‑19 (MQ=255)                             acacCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttg                                                                                                                                                                                                           >  1:656721‑M1/1‑231 (MQ=255)                             acacCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaa                                                                                                                                                                                                               >  1:840416‑M1/1‑231 (MQ=255)                                  aTCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtg                                                                                                                                                                                                      >  1:576972‑M1/1‑226 (MQ=255)                                  aTCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatt                                                                                                                                                                                                             >  1:735215‑M1/1‑226 (MQ=255)                                       ggTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacgga                                                                                                                                                                                                                      >  1:498242‑M1/1‑221 (MQ=255)                                        gTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagatt                                                                                                                                                                                                >  1:555999‑M1/1‑220 (MQ=255)                                             tGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtc                                                                                                                                                                                           <  1:846066‑M1/251‑37 (MQ=255)                                              gAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCAc                                                                                                                                                                                                                                         >  1:715642/1‑204 (MQ=255)                                              gAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAAcc                                                                                                                                                                                                                                >  1:803618/1‑213 (MQ=255)                                                gCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtctta                                                                                                                                                                                        >  1:294978‑M1/1‑212 (MQ=255)                                                   tAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactc                                                                                                                                                                                     <  1:834850‑M1/251‑43 (MQ=255)                                                   tAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactc                                                                                                                                                                                     <  1:570445‑M1/251‑43 (MQ=255)                                                   tAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactc                                                                                                                                                                                     <  1:876‑M1/251‑43 (MQ=255)                                                    aaTTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAAcc                                                                                                                                                                                                                                <  1:262145/207‑1 (MQ=255)                                                     aTTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAAc                                                                                                                                                                                                                                 >  1:601904/1‑205 (MQ=255)                                                     aTTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactctt                                                                                                                                                                                   >  1:93332‑M1/1‑207 (MQ=255)                                                     aTTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactctt                                                                                                                                                                                   >  1:195506‑M1/1‑207 (MQ=255)                                                     aTTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactc                                                                                                                                                                                     >  1:103016‑M1/1‑207 (MQ=255)                                                        tAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttacc                                                                                                                                                                                <  1:776130‑M1/251‑48 (MQ=255)                                                               gATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcag                                                                                                                                                                                                   <  1:559060‑M1/225‑29 (MQ=255)                                                                                ggATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaa                                                                                                                                                         >  1:261979‑M1/1‑180 (MQ=255)                                                                                ggATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaa                                                                                                                                                         >  1:792303‑M1/1‑180 (MQ=255)                                                                                ggATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaa                                                                                                                                                         >  1:163099‑M1/1‑180 (MQ=255)                                                                                ggATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaa                                                                                                                                                         >  1:211211‑M1/1‑180 (MQ=255)                                                                                ggATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgagg                                                                                                                                                                                                        >  1:420304‑M1/1‑180 (MQ=255)                                                                                 gATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaac                                                                                                                                                        <  1:98741‑M1/250‑72 (MQ=255)                                                                                 gATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaac                                                                                                                                                        <  1:470985‑M1/250‑72 (MQ=255)                                                                                  aTTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacag                                                                                                                                                      <  1:699774‑M1/251‑74 (MQ=255)                                                                                  aTTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacgga                                                                                                                                                                                                                      >  1:528774‑M1/1‑178 (MQ=255)                                                                                        gATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaa                                                                                                                                                          >  1:896379‑M1/1‑172 (MQ=255)                                                                                         aTCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccg                                                                                                                                               >  1:492406‑M1/1‑171 (MQ=255)                                                                                         aTCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccg                                                                                                                                               <  1:625345‑M1/251‑81 (MQ=255)                                                                                               gCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtc                                                                                                                                                                                           >  1:826896‑M1/1‑165 (MQ=255)                                                                                               gCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtc                                                                                                                                                                                           <  1:133237‑M1/201‑37 (MQ=255)                                                                                                 ccTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggat                                                                                                                                       <  1:711596‑M1/251‑89 (MQ=255)                                                                                                 ccTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattat                                                                                                                                                                                              >  1:711461‑M1/1‑163 (MQ=255)                                                                                                 ccTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatt                                                                                                                                                                                                             >  1:524890‑M1/1‑163 (MQ=255)                                                                                                    gAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGc                                                                                                                                                                                                                                           >  1:31167/1‑148 (MQ=255)                                                                                                    gAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggatttt                                                                                                                                    >  1:385843‑M1/1‑160 (MQ=255)                                                                                                        gcggcAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggattttggt                                                                                                                                 <  1:891314‑M1/250‑95 (MQ=255)                                                                                                                         cAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtc                                                                                                                                                                                           <  1:198295‑M1/175‑37 (MQ=255)                                                                                                                          aaaTTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactc                                                                                                                                                                                     >  1:764254‑M1/1‑138 (MQ=255)                                                                                                                           aaTTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggattttggtatccgtgaggtggcgaaact                                                                                                             <  1:211689‑M1/251‑115 (MQ=255)                                                                                                                                            cacCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAGGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGtt                                                                                            >  1:275174/1‑251 (MQ=21)                                                                                                                                            cacCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGtt                                                                                            >  1:215795/1‑251 (MQ=21)                                                                                                                                              ccGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggac                                                                                                                                                                                                                           >  1:637189‑M1/1‑118 (MQ=255)                                                                                                                                                          gCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggattttgg                                                                                                                                  >  1:665739‑M1/1‑106 (MQ=255)                                                                                                                                                          gCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTAt                                                                              >  1:341581/1‑251 (MQ=21)                                                                                                                                                              aaaTGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGa                                                                          <  1:56730/251‑1 (MQ=18)                                                                                                                                                                      ggCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcacc                                                                                                                                                <  1:464158‑M1/173‑80 (MQ=255)                                                                                                                                                                      ggCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGcc                                                                    <  1:130955/249‑1 (MQ=18)                                                                                                                                                                      ggCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCtt                                                                  >  1:858432/1‑251 (MQ=18)                                                                                                                                                                       gCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacgga                                                                                                                                                                                                                      <  1:195040‑M1/102‑10 (MQ=255)                                                                                                                                                                        cTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatt                                                                                                                                                                                                             <  1:854073‑M1/110‑19 (MQ=255)                                                                                                                                                                            cacaCCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGcc                                                                    >  1:68607/1‑243 (MQ=18)                                                                                                                                                                             acacCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGaa                                                           <  1:580042/251‑1 (MQ=18)                                                                                                                                                                                       cTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggattttggta                                                                                                                                <  1:728117‑M1/172‑96 (MQ=255)                                                                                                                                                                                         tCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggattttggtatccgtgaggtggcgaaacttcatcag                                                                                                      >  1:110595‑M1/1‑75 (MQ=255)                                                                                                                                                                                         tCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                               >  1:26391/1‑251 (MQ=17)                                                                                                                                                                                                                  cTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                      <  1:254574/251‑1 (MQ=14)                                                                                                                                                                                                                  cTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                      <  1:427356/251‑1 (MQ=14)                                                                                                                                                                                                                                aatttcaaCCGCACTTTGCTCTGCACCCCAAAACCT‑‑‑‑‑‑GGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTg  <  1:688941/244‑1 (MQ=2)                                                                                                                                                                                                                                aatttcaaCCGCACTTTGCTCTGCACCCCAAAACCT‑‑‑‑‑‑GGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTg  <  1:336185/244‑1 (MQ=2)                                                                                                                                                                                                                                  ttCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt      <  1:828780/251‑1 (MQ=11)                                                                                                                                                                                                                                  ttCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt      <  1:185103/251‑1 (MQ=11)                                                                                                                                                                                                                                          gCACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaactatcaaacaggtcaccgagcaggat                                                                                                                                       >  1:300011‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                          gCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                           >  1:503364/1‑222 (MQ=11)                                                                                                                                                                                                                                           cACTTTGATCTGCACCCCAAAACCTggacacggacacctaatttgaggtgcagattatgtcttactc                                                                                                                                                                                     <  1:273890‑M1/67‑43 (MQ=255)                                                                                                                                                                                                                                           cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAccc                     <  1:9422/227‑1 (MQ=1)                                                                                                                                                                                                                                           cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg    >  1:516002/1‑244 (MQ=1)                                                                                                                                                                                                                                           cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACAAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCAGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGCAAGAGGGCTTAAt                                               >  1:190524/1‑201 (MQ=255)                                                                                                                                                                                                                                               ttGCTCTGCACCCCAAAACCT‑‑‑‑‑‑GGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                             <  1:325746/193‑1 (MQ=255)                                                                                                                                                                                                                                                 gCTCTGCACCCCAAAACCT‑‑‑‑‑‑GGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg    >  1:256004/1‑232 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               GTGTTACACCGTTTACGCGATCAAGGCAACACCATCGTGGTCATTGAGCATAATTTAGACGTGATTAAAACCGCCGACTGGATTGTAGATCTCGGCCCTGAAGGCGGCAGCGGCGGCGGACAAATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGCTCTGCACCCCAAAACCT‑‑‑‑‑‑GGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTG  >  1/593210‑593683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑