Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	572,184	T→C	intergenic (+74631/‑127159)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	572,184	0	T	C	100.0%	214.4 / NA	74	intergenic (+74631/‑127159)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/31);  total (43/31)

ATTTAGATACAATCAAAAATTATAATTTCGCTTGTCCACAAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATTCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTACAGCCCTGCAGAAAAAAGTCTTGGCATTTTATGCG  >  1/571942‑572426                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   aTTTAGATACAATCAAAAATTATAATTTCGCTTGTCCACAAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTc                                                                                                                                                                                                                                             >  1:77232/1‑250 (MQ=255)                    ttATAATTTCGCTTGTCCACAAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATg                                                                                                                                                                                                                         >  1:150499/1‑251 (MQ=255)                                 tGTCCACAAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTtgtg                                                                                                                                                                                                              >  1:520413/1‑249 (MQ=255)                                    ccACAAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTaa                                                                                                                                                                                                          >  1:70100/1‑250 (MQ=255)                                       caAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACaaa                                                                                                                                                                                                      >  1:31286/1‑251 (MQ=255)                                         aaaTGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATa                                                                                                                                                                                                    >  1:458920/1‑251 (MQ=255)                                         aaaTGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATa                                                                                                                                                                                                    >  1:179787/1‑251 (MQ=255)                                         aaaTGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATa                                                                                                                                                                                                    >  1:194170/1‑251 (MQ=255)                                         aaaTGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATa                                                                                                                                                                                                    >  1:194858/1‑251 (MQ=255)                                              acacaacacaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGaa                                                                                                                                                                                                                             <  1:728300/221‑1 (MQ=255)                                              acacaacacaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAAt                                                                                                                                                                                                                          >  1:602960/1‑224 (MQ=255)                                                 caacgcaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACgaaagaa                                                                                                                                                                                            >  1:59233/1‑251 (MQ=255)                                                   acacaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGaaagaaag                                                                                                                                                                                          >  1:234674/1‑251 (MQ=255)                                                   acacaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGaaagaaag                                                                                                                                                                                          >  1:43043/1‑251 (MQ=255)                                                    cacaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGt                                                                                                                                                                                         >  1:854254/1‑251 (MQ=255)                                                     acaTATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTg                                                                                                                                                                                        >  1:821223/1‑251 (MQ=255)                                                          tttCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGaa                                                                                                                                                                                                                             >  1:873141/1‑209 (MQ=255)                                                                   atTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCgaa                                                                                                                                                                          >  1:478542/1‑251 (MQ=255)                                                                   atTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCgaa                                                                                                                                                                          >  1:820389/1‑251 (MQ=255)                                                                                 gCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGc                                                                                                                                                                                                             <  1:548333/202‑1 (MQ=255)                                                                                  ccATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTa                                                                                                                                                           <  1:564084/251‑1 (MQ=255)                                                                                          gCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATaa                                                                                                                                                   >  1:19507/1‑251 (MQ=255)                                                                                           cTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGaa                                                                                                                                                                                                                             >  1:77900/1‑176 (MQ=255)                                                                                            ttATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACa                                                                                                                                                 <  1:339408/251‑1 (MQ=255)                                                                                             tATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAAt                                                                                                                                                                                                                          >  1:612269/1‑177 (MQ=255)                                                                                             tATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACaa                                                                                                                                                <  1:649399/251‑1 (MQ=255)                                                                                              aTTTAGATCCAAAGGCTGTGGGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACaa                                                                                                                                                <  1:685330/251‑1 (MQ=255)                                                                                                   gATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCa                                                                                                                                          >  1:199084/1‑251 (MQ=255)                                                                                                     tCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGaattaatt                                                                                                                                                                 >  1:512801/1‑226 (MQ=255)                                                                                                     tCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTAtgt                                                                                                                                                        <  1:210608/235‑1 (MQ=255)                                                                                                     tCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGa                                                                                                                                        >  1:239009/1‑251 (MQ=255)                                                                                                     tCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATATACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGa                                                                                                                                        >  1:430378/1‑251 (MQ=255)                                                                                                           ggCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTc                                                                                                                                                               >  1:269321/1‑222 (MQ=255)                                                                                                           ggCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAAt                                                                                                                                  >  1:817525/1‑251 (MQ=255)                                                                                                            gCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAAtt                                                                                                                                 >  1:750121/1‑251 (MQ=255)                                                                                                                  ggAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGAt                                                                                                                                                     >  1:632909/1‑226 (MQ=255)                                                                                                                   gAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCAc                                                                                                                            >  1:86012/1‑250 (MQ=255)                                                                                                                         gCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTc                                                                                                                                                               >  1:799337/1‑209 (MQ=255)                                                                                                                                    tCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGaa                                                                                                                                                                                                                             >  1:173931/1‑136 (MQ=255)                                                                                                                                       ggAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAg                                                                                                                                                                                                                               <  1:613100/131‑1 (MQ=255)                                                                                                                                        gAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGaa                                                                                                        <  1:684000/249‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACtt                                                                                                                                                                                  <  1:798794/171‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTc                                                                                                                                           <  1:111523/210‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCAcc                                                                                                                           <  1:413531/226‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCAcc                                                                                                                           <  1:437513/226‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAgg                                                                                                          <  1:569020/243‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGaa                                                                                                        <  1:625234/245‑1 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAatatat                                                                                                   >  1:424383/1‑250 (MQ=255)                                                                                                                                            ccATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAatatat                                                                                                   <  1:225572/250‑1 (MQ=255)                                                                                                                                             cATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATcc                                                                                                 <  1:626424/251‑1 (MQ=255)                                                                                                                                                tAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGc                                                                                              <  1:624923/251‑1 (MQ=255)                                                                                                                                                  gATTTGGAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGaattaatt                                                                                                                                                                 >  1:573028/1‑182 (MQ=255)                                                                                                                                                  gATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCAc                                                                                                                                                                                                                                                 >  1:777872/1‑102 (MQ=255)                                                                                                                                                            gCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCgaag                                                                                                                                                                         <  1:160717/164‑1 (MQ=255)                                                                                                                                                            gCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTttctt                                                                                     >  1:51439/1‑248 (MQ=255)                                                                                                                                                                        tAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGTAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAatta                                                                      <  1:382897/251‑1 (MQ=255)                                                                                                                                                                        tAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAAt                                                                                                                                                                                                                          >  1:343536/1‑103 (MQ=255)                                                                                                                                                                         aaaGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAattat                                                                     <  1:788428/251‑1 (MQ=255)                                                                                                                                                                           aGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAgg                                                                                                          <  1:94839/212‑1 (MQ=255)                                                                                                                                                                                     ttATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAg                                                         <  1:159201/251‑1 (MQ=255)                                                                                                                                                                                      tATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAGTCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGc                                                        <  1:258335/251‑1 (MQ=255)                                                                                                                                                                                            cACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAAc                                                   <  1:541774/250‑1 (MQ=255)                                                                                                                                                                                              ccTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTAt                                                                                                                                                          >  1:779148/1‑145 (MQ=255)                                                                                                                                                                                              ccTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGaa                                                                                                        <  1:165382/195‑1 (MQ=255)                                                                                                                                                                                              ccTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGt                                                >  1:145785/1‑251 (MQ=255)                                                                                                                                                                                                        gTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTg                                        >  1:593667/1‑249 (MQ=255)                                                                                                                                                                                                          aCCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTAc                                    <  1:716859/251‑1 (MQ=255)                                                                                                                                                                                                          aCCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTAc                                    <  1:43978/251‑1 (MQ=255)                                                                                                                                                                                                               aaaaaTTCCGGCAAGCGAATTTGCCGAATTATTAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAATAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTACAGcc                                <  1:447687/250‑1 (MQ=255)                                                                                                                                                                                                                                          attattAAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTACAGCCCTGCAGAAAAAAGTCTTGGCATTTTATg    <  1:347520/251‑1 (MQ=255)                                                                                                                                                                                                                                           ttattaAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAGGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTACAGCCCTGCAGAAAAAAGTCTTGGCATTTTATg    >  1:135097/1‑250 (MQ=255)                                                                                                                                                                                                                                             attaAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAAcc                                                                                                                    >  1:85889/1‑136 (MQ=255)                                                                                                                                                                                                                                             attaAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAgg                                                                                                          <  1:100035/146‑1 (MQ=255)                                                                                                                                                                                                                                             attaAATCCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGGTTTTATTATTAGTGTTGATAGCGGAACGGGTCATCTTGCTACAACCCTGCAGAAAAAAGTCTTGGCATTTTAttcg  >  1:273522/1‑247 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ATTTAGATACAATCAAAAATTATAATTTCGCTTGTCCACAAAATGACACAACACATATTTCTGAATATTTGAATGATTCTGCCATTTCTGCTTATTTAGATCCAAAGGCTGT‑GGAATATGCTTTAATAAATCAGGAAACCATTAGATTTGAAGTGCTTTGGGGCGATAAAGTGAGAATTTTATTGGCACCTGAAGGCAGTACCAGAAAAATTCCGGCAAGCGAATTTGCCGAATTATTAAATTCACTTTCCCCAACGATTGTAGAAAATGTACTTTTTGTGCTAACAAATACGAAAGAAAGTGCCACTTATTTCGAAGAATTAATTTCATTATGTGATAACAAAATTCAGATAAAATTATCACCTAAAACCACAATTCAGGAATATATCCAGCTTGTTTCTTCTGCGGATTTAATTATTAGTGTTGATAGCGGAACGGTTCATCTTGCTACAGCCCTGCAGAAAAAAGTCTTGGCATTTTATGCG  >  1/571942‑572426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑