Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	500,228	T→C	intergenic (+2675/‑199115)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	500,228	0	T	C	100.0%	212.6 / NA	77	intergenic (+2675/‑199115)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/42);  total (35/42)

ACCTTTAATGTGCAGCACGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACGATAAATTTCATCCAGAATTTCTTGTCCTGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAGAATGTAAATGAATTTTAATTAG  >  1/499988‑500468                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 aCCTTTAATGTGCAGCACGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCAt                                                                                                                                                                                                                                        >  1:384251/1‑251 (MQ=255)  ccTTTAATGTGCAGCACGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCAtt                                                                                                                                                                                                                                       >  1:831100/1‑251 (MQ=255)        aTGTGCAGCACGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCa                                                                                                                                                                                                                                 >  1:549124/1‑251 (MQ=255)            gcagcaCGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATACATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATaa                                                                                                                                                                                                                             >  1:188967/1‑251 (MQ=255)             cagcaCGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATaa                                                                                                                                                                                                                             <  1:495148/250‑1 (MQ=255)                     cGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTc                                                                                                                                                                                                                    <  1:768765/251‑1 (MQ=255)                          ccTTCGCGGGTGTTAATCGGTCCGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACaat                                                                                                                                                                                                               <  1:11290/250‑1 (MQ=255)                           cTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACaata                                                                                                                                                                                                              >  1:355807/1‑251 (MQ=255)                           cTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACaata                                                                                                                                                                                                              >  1:665799/1‑251 (MQ=255)                                cgGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCcg                                                                                                                                                                                                         <  1:286991/251‑1 (MQ=255)                                  ggTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCcgcg                                                                                                                                                                                                       >  1:595474/1‑251 (MQ=255)                                  ggTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCcgcg                                                                                                                                                                                                       >  1:845834/1‑251 (MQ=255)                                   gtgtTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACaat                                                                                                                                                                                                               >  1:163252/1‑242 (MQ=255)                                                  ccGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGt                                                                                                                                                                                       >  1:560976/1‑251 (MQ=255)                                                       gaaCATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAg                                                                                                                                                                                    >  1:446268/1‑249 (MQ=255)                                                        aaCATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGc                                                                                                                                                                                   <  1:247162/249‑1 (MQ=255)                                                            tatGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCcgc                                                                                                                                                                                                        <  1:831093/224‑1 (MQ=255)                                                                  gATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCg                                                                                                                                                                       >  1:22409/1‑251 (MQ=255)                                                                    tCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACaat                                                                                                                                                                                                               <  1:218768/209‑1 (MQ=255)                                                                               ttGATATTTCTGGCGGTTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGc                                                                                                                                                            <  1:737039/249‑1 (MQ=255)                                                                                tGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGcc                                                                                                                                                                                                                                  <  1:347419/178‑1 (MQ=255)                                                                                            cGGGTTTCGTAGTCCCAAACAGCATTGGTGGCGCTTTTCCCCGCCCCCACCAAATTGCGTTGCGCCCGATGCCAATCAATTAAATTTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCAAAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTataaat                                                                                                                                             <  1:550862/251‑1 (MQ=255)                                                                                               gTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTAtaaataaa                                                                                                                                          >  1:154989/1‑251 (MQ=255)                                                                                                  tCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAg                                                                                                                                                                                                                           >  1:860575/1‑167 (MQ=255)                                                                                                  tCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAAtt                                                                                                                                        >  1:465067/1‑250 (MQ=255)                                                                                                  tCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAAtt                                                                                                                                        >  1:740464/1‑250 (MQ=255)                                                                                                  tCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTc                                                                                                                                       <  1:618105/251‑1 (MQ=255)                                                                                                     tagtaCCAATCAGCATTGGTGGCGCTTTTCTCCGCCCCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGTTTTATCCTGCTTCCCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCAc                                                                                                                                    <  1:487230/251‑1 (MQ=255)                                                                                                     tagtaCCAATCAGCATTGGTGGCGCTTTGCTCCGCACCCAAAAAATTGCGTTGCGCCCGTTGCCAATCAATTAAAATTTTCACTTCCAGTTCCGTTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCAc                                                                                                                                    <  1:583666/251‑1 (MQ=255)                                                                                                       gtaCCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACtt                                                                                                                                  >  1:611735/1‑251 (MQ=255)                                                                                                                     tggtggCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAAt                                                                                                                    <  1:309393/251‑1 (MQ=255)                                                                                                                        tggCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCCCTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATgg                                                                                                                  <  1:65182/250‑1 (MQ=255)                                                                                                                           cgcTTTTCTCCGCCCCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAAttt                                                                                                                                                                         <  1:366273/192‑1 (MQ=255)                                                                                                                               tttCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGcc                                                                                                                                                                                                                                               >  1:639994/1‑118 (MQ=255)                                                                                                                                 tctcCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATgg                                                                                                         >  1:67569/1‑250 (MQ=255)                                                                                                                                 tctcCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGc                                                                                                        <  1:118725/251‑1 (MQ=255)                                                                                                                                  ctcCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGaat                                                                                                                                                                              <  1:330593/180‑1 (MQ=255)                                                                                                                                  ctcCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCa                                                                                                       <  1:401278/251‑1 (MQ=255)                                                                                                                                         cccAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCttt                                                                                                 >  1:593368/1‑250 (MQ=255)                                                                                                                                            aacaaATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGaataa                                                                                                                                                                            <  1:877220/172‑1 (MQ=255)                                                                                                                                            aacaaATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAg                                                                                             <  1:659597/251‑1 (MQ=255)                                                                                                                                             acaaATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGa                                                                                            <  1:51143/251‑1 (MQ=255)                                                                                                                                               aaaTTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAgg                                                                                                                                                             >  1:46186/1‑184 (MQ=255)                                                                                                                                                        tgcgCCCGATGCCAATCAATAAAAATTTTCCCTTCCATTTCCGGTTTACCCTGCTTCGCAAAATAAATTTCATCCAGAATTCCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTgcgcgc                                                                                 <  1:379910/251‑1 (MQ=255)                                                                                                                                                           gcCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACg                                                                              >  1:258674/1‑251 (MQ=255)                                                                                                                                                           gcCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACg                                                                              >  1:150159/1‑251 (MQ=255)                                                                                                                                                              cGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTgcgcgc                                                                                 <  1:872530/245‑1 (MQ=255)                                                                                                                                                                aTGCCAATCAATTAAAATTTTCCCTTCAAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCT‑‑‑AATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGAtt                                                                       <  1:840141/250‑1 (MQ=255)                                                                                                                                                                aTGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGt                                                                                                                                                                                                      >  1:275873/1‑126 (MQ=255)                                                                                                                                                                aTGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAACCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGa                                                                         >  1:445243/1‑251 (MQ=255)                                                                                                                                                                aTGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAACCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGa                                                                         >  1:429848/1‑251 (MQ=255)                                                                                                                                                                   ccAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGAtt                                                                       <  1:264956/250‑1 (MQ=255)                                                                                                                                                                       tcaatTAAAATTTTCCCTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATc                                                                  <  1:17883/251‑1 (MQ=255)                                                                                                                                                                                aTTTTCACTTCCATTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACAt                                                         <  1:800015/251‑1 (MQ=255)                                                                                                                                                                                aTTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACAt                                                         >  1:584151/1‑251 (MQ=255)                                                                                                                                                                                     ccctcccAGTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAAtt                                                    <  1:261976/246‑1 (MQ=255)                                                                                                                                                                                     cACTTCCATTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAAtt                                                    <  1:732457/251‑1 (MQ=255)                                                                                                                                                                                             gTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTg                                                                                                                                                                                                                         <  1:778082/78‑1 (MQ=255)                                                                                                                                                                                             gTTCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTc                                                                                                                                       >  1:283467/1‑160 (MQ=255)                                                                                                                                                                                               tCCGGTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCtat                                          <  1:659303/251‑1 (MQ=255)                                                                                                                                                                                               tCCGGTTTATCCTGCTTCGCACAATAAACTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCtat                                          <  1:428292/251‑1 (MQ=255)                                                                                                                                                                                                  ggTTTATCCTGCTTCGCACAATAAATTTCATCCAGATTTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTtc                                               <  1:122133/243‑1 (MQ=255)                                                                                                                                                                                                  ggTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTg                                                                                                                                 <  1:196590/161‑1 (MQ=255)                                                                                                                                                                                                  ggTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCa                                                                                                                <  1:579911/178‑1 (MQ=255)                                                                                                                                                                                                  ggTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTaa                                                             >  1:555020/1‑229 (MQ=255)                                                                                                                                                                                                  ggTTTATCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAAtt                                                    >  1:358345/1‑238 (MQ=255)                                                                                                                                                                                                   ttttATCCTGCTTCGCACAATAAATTTCATCCAAAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGC‑TTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATaaaaa                                     <  1:754745/250‑1 (MQ=255)                                                                                                                                                                                                       aTCCTGCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAAtt                                                    <  1:366416/233‑1 (MQ=255)                                                                                                                                                                                                            gCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGa                             >  1:343502/1‑251 (MQ=255)                                                                                                                                                                                                            gCTTCGCACAATAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGa                             >  1:734931/1‑251 (MQ=255)                                                                                                                                                                                                                   acaataatttccacaaaGAATTTCTTTTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAGaa                      <  1:540515/235‑1 (MQ=255)                                                                                                                                                                                                                   acaaTAAATTCCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAGaa                      <  1:511629/248‑1 (MQ=255)                                                                                                                                                                                                                    caaTAAATTTCATCCAGAATTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTataaat                                                                                                                                             <  1:518791/129‑1 (MQ=255)                                                                                                                                                                                                                                      aTTTCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAGAATGTAAATGAATTTTAATTa   >  1:351157/1‑251 (MQ=255)                                                                                                                                                                                                                                        tttttGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAg                        <  1:835187/225‑1 (MQ=255)                                                                                                                                                                                                                                        ttCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAg                        >  1:360056/1‑228 (MQ=255)                                                                                                                                                                                                                                        ttCTTGTCCCGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAGAATGTAAATGAATTTTAATTAg  >  1:563566/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ACCTTTAATGTGCAGCACGCCGAACACTTCGCGGGTGTTAATCGGTACGCCGAAGAACATATGCGGATCTTCCGCGAGTTGATATTTCTGGCGGGTTTCGTAGTACCAATCAGCATTGGTGGCGCTTTTCTCCGCACCCAACAAATTGCGTTGCGCCCGATGCCAATCAATTAAAATTTTCACTTCCAGTTCCGGTTTATCCTGCTTCGCACGATAAATTTCATCCAGAATTTCTTGTCCTGCCTCGTCATTTTGCCAATAAAGTGCGGTCACAATAATCCGCGTTTTTGCTTGTCGAGTCAGCTGAATAATTTCGCTTTTAAAGGCAGCCGGGCTATAAATAAATTCCACTTGCTCCGGCTGTAATGGCAAACATGGCAGGCTTTCAAGACGTTTGCGCGCACGTTCGATTTTATCTGTAAACATAAATTAGTTCTCTATAAAAACGCTGTGAGTAAGAATGTAAATGAATTTTAATTAG  >  1/499988‑500468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑