Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	480,426	A→T	intergenic (‑63604/‑17037)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	480,426	0	A	T	100.0%	197.5 / NA	70	intergenic (‑63604/‑17037)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (42/28);  total (42/28)

ACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATAGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACAC  >  1/480189‑480668                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   aCCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCg                                                                                                                                                                                                                                       >  1:225840/1‑251 (MQ=255)     aCGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATa                                                                                                                                                                                                                                   >  1:735966/1‑251 (MQ=255)     aCGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCATTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATa                                                                                                                                                                                                                                   >  1:771855/1‑251 (MQ=255)          aCCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGa                                                                                                                                                                                                                              >  1:588470/1‑251 (MQ=255)          aCCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGa                                                                                                                                                                                                                              >  1:306234/1‑251 (MQ=255)                 ataatGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAAt                                                                                                                                                                                                                       <  1:196365/251‑1 (MQ=255)                               cACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCAc                                                                                                                                                                                                         <  1:517014/251‑1 (MQ=255)                                           cggcggGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGt                                                                                                                                                                                             <  1:95053/251‑1 (MQ=255)                                           cggcggGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGt                                                                                                                                                                                             <  1:568093/251‑1 (MQ=255)                                                 gAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCg                                                                                                                                                                                        <  1:804565/250‑1 (MQ=255)                                                                     ccATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGcc                                                                                                                                                                    <  1:895277/250‑1 (MQ=255)                                                                     ccATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCt                                                                                                                                                                   <  1:277949/251‑1 (MQ=255)                                                                        tttCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGcc                                                                                                                                                                                                                                               >  1:125979/1‑172 (MQ=255)                                                                          tCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGc                                                                                                                                                              <  1:468775/251‑1 (MQ=255)                                                                               tGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGATCCGTTCCAAATCATGCCTAGTGCTACtt                                                                                                                                                         <  1:237064/251‑1 (MQ=255)                                                                               tGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACtt                                                                                                                                                         <  1:235802/251‑1 (MQ=255)                                                                                        ggCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATg                                                                                                                                                                                              >  1:600175/1‑205 (MQ=255)                                                                                        ggCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGt                                                                                                                                                                                             >  1:865442/1‑206 (MQ=255)                                                                                        ggCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGca                                                                                                                                                >  1:667113/1‑251 (MQ=255)                                                                                          cTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGcaca                                                                                                                                              >  1:630094/1‑251 (MQ=255)                                                                                                  ccGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCAc                                                                                                                                      <  1:289773/251‑1 (MQ=255)                                                                                                    gttgttTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACgg                                                                                                                                                                                                       >  1:797270/1‑184 (MQ=255)                                                                                                           ggCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCa                                                                                                                                       <  1:651357/241‑1 (MQ=255)                                                                                                           ggCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGa                                                                                                                             >  1:827599/1‑251 (MQ=255)                                                                                                            gCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAg                                                                                                                            >  1:644979/1‑251 (MQ=255)                                                                                                            gCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAg                                                                                                                            >  1:629766/1‑251 (MQ=255)                                                                                                                 gAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGt                                                                                                                                                                                             >  1:570535/1‑181 (MQ=255)                                                                                                                 gAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTc                                                                                                                                                                               <  1:732462/195‑1 (MQ=255)                                                                                                                 gAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTAc                                                                                                                                                           >  1:368209/1‑215 (MQ=255)                                                                                                                 gAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGa                                                                                                                       >  1:216904/1‑251 (MQ=255)                                                                                                                 gAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACAGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGa                                                                                                                       >  1:443304/1‑251 (MQ=255)                                                                                                                     aGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCt                                                                                                                                                                   <  1:470350/203‑1 (MQ=255)                                                                                                                      gCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCAc                                                                                                                                      <  1:897359/231‑1 (MQ=255)                                                                                                                               ttCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACtt                                                                                                                                                         >  1:802654/1‑203 (MQ=255)                                                                                                                               ttCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGc                                                                                                                                        >  1:579097/1‑220 (MQ=255)                                                                                                                               ttCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGa                                                                                                         >  1:39684/1‑251 (MQ=255)                                                                                                                               ttCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTAAAAAACGGCGa                                                                                                         >  1:796433/1‑251 (MQ=255)                                                                                                                                         aCTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCaa                                                                                               >  1:216098/1‑251 (MQ=255)                                                                                                                                               gCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAAtt                                                                                          <  1:647245/250‑1 (MQ=255)                                                                                                                                                   tttCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGt                                                                                                                                                                                             <  1:453293/147‑1 (MQ=255)                                                                                                                                                   tttCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTc                                                                                      >  1:549058/1‑250 (MQ=255)                                                                                                                                                   tttCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCAGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTc                                                                                      >  1:762051/1‑250 (MQ=255)                                                                                                                                                    ttCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAg                                                                                                                                          <  1:242826/197‑1 (MQ=255)                                                                                                                                                    ttCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCa                                                                                    >  1:618547/1‑251 (MQ=255)                                                                                                                                                          aCGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACtt                                                                                                                                                         <  1:820699/176‑1 (MQ=255)                                                                                                                                                             tAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGt                                                                                                                                                                                             >  1:130007/1‑137 (MQ=255)                                                                                                                                                              aGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTa                                                                          >  1:803391/1‑251 (MQ=255)                                                                                                                                                               gCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAAtt                                                                                          >  1:679477/1‑234 (MQ=255)                                                                                                                                                                   gAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAagc                                                                       <  1:138224/249‑1 (MQ=255)                                                                                                                                                                   gAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAagc                                                                       <  1:664197/249‑1 (MQ=255)                                                                                                                                                                                ttATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAAcc                                                                                                                                                                                   >  1:435617/1‑128 (MQ=255)                                                                                                                                                                                ttATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACtt                                                                                                                                                         <  1:113265/154‑1 (MQ=255)                                                                                                                                                                                     ggCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGt                                                   >  1:786585/1‑251 (MQ=255)                                                                                                                                                                                            tCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTggtggt                                            <  1:422838/251‑1 (MQ=255)                                                                                                                                                                                                             tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAAt                                                                                                                                                                          <  1:734302/108‑1 (MQ=255)                                                                                                                                                                                                             tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCg                                                                                                                              <  1:687467/152‑1 (MQ=255)                                                                                                                                                                                                             tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGt                                                   >  1:786558/1‑227 (MQ=255)                                                                                                                                                                                                             tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGt                           >  1:7405/1‑251 (MQ=255)                                                                                                                                                                                                             tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGt                           >  1:196755/1‑251 (MQ=255)                                                                                                                                                                                                             tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGt                           >  1:310730/1‑251 (MQ=255)                                                                                                                                                                                                                  gAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCcaa                       >  1:354215/1‑250 (MQ=255)                                                                                                                                                                                                                    aTAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCcaacaa                    <  1:750710/251‑1 (MQ=255)                                                                                                                                                                                                                    aTAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCcaacaa                    >  1:705371/1‑251 (MQ=255)                                                                                                                                                                                                                     tAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAAt                   <  1:576221/251‑1 (MQ=255)                                                                                                                                                                                                                       gCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATaa                 >  1:527358/1‑251 (MQ=255)                                                                                                                                                                                                                       gCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATaa                 >  1:554383/1‑251 (MQ=255)                                                                                                                                                                                                                          tAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGCTTTTCCGTCCAACAATAACg               >  1:664192/1‑250 (MQ=255)                                                                                                                                                                                                                          tAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACg               >  1:272013/1‑250 (MQ=255)                                                                                                                                                                                                                          tAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACg               >  1:48212/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaaaTTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAacac  <  1:554386/248‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   ACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACGTAGCAGGAAATTGATAAATTTATAGGCGTTTTCGACGTTTTTCGCGCTTTTCGGAATAGCGTAGTTATCCATCCAGAAAATAGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACAC  >  1/480189‑480668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑