Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	480,106	A→G	intergenic (‑63284/‑17357)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	480,106	0	A	G	100.0%	171.0 / NA	62	intergenic (‑63284/‑17357)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/31);  total (31/31)

ACAACCGACAGCGGTTATTGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTAAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACG  >  1/479861‑480344                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               aCAACCGACAGCGGTTATTGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTgaaaaa                                                                                                                                                                                                                                           >  1:122891/1‑251 (MQ=255)            cGGTTATTGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCAc                                                                                                                                                                                                                                  <  1:187045/249‑1 (MQ=255)            cGGTTATTGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACtt                                                                                                                                                                                                                                >  1:797638/1‑251 (MQ=255)                  ttGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          <  1:487814/251‑1 (MQ=255)                  ttGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          <  1:428638/251‑1 (MQ=255)                  ttGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          <  1:290684/251‑1 (MQ=255)                  ttGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          <  1:595087/251‑1 (MQ=255)                    gTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttatta                                                                                                                                                                                                                        >  1:548598/1‑251 (MQ=255)                    gTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttatta                                                                                                                                                                                                                        >  1:512166/1‑251 (MQ=255)                     tCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttatta                                                                                                                                                                                                                        <  1:535122/250‑1 (MQ=255)                       cTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATcc                                                                                                                                                                                                                     <  1:552988/251‑1 (MQ=255)                            ggATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          >  1:86784/1‑241 (MQ=255)                                 tCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAacc                                                                                                                                                                                                                                         <  1:417103/221‑1 (MQ=255)                                 tCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTaattaatt                                                                                                                                                                                                           <  1:719836/251‑1 (MQ=255)                                 tCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTaattaatt                                                                                                                                                                                                           >  1:538974/1‑251 (MQ=255)                                    cTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAg                                                                                                                                                                                                         <  1:302092/250‑1 (MQ=255)                                             gCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttatta                                                                                                                                                                                                                        >  1:295353/1‑226 (MQ=255)                                              ccAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACtt                                                                                                                                                                                               <  1:613983/250‑1 (MQ=255)                                                 aGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          >  1:176635/1‑220 (MQ=255)                                                               aaCGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAg                                                                                                                                                                                                         <  1:205379/223‑1 (MQ=255)                                                                   cATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          >  1:548758/1‑202 (MQ=255)                                                                                   aaaCGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCt                                                                                                                                                          <  1:818403/250‑1 (MQ=255)                                                                                       gTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGt                                                                                                                                                      >  1:209917/1‑250 (MQ=255)                                                                                        tGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTc                                                                                                                                                     <  1:835332/250‑1 (MQ=255)                                                                                            gaagaaAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGc                                                                                                                                                                 >  1:320020/1‑234 (MQ=255)                                                                                                 ggctcggagagtgtataagagacaggCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGaat                                                                                                                                            <  1:529822/225‑1 (MQ=255)                                                                                                 aaaCATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTAcc                                                                                                                                                           <  1:664769/235‑1 (MQ=255)                                                                                                  aaCATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGaataa                                                                                                                                          <  1:464337/251‑1 (MQ=255)                                                                                                        cGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCAt                                                                                                                                                                              >  1:495159/1‑209 (MQ=255)                                                                                                             gCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGaat                                                                                                                                            >  1:452735/1‑238 (MQ=255)                                                                                                                   ccacGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGCGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGt                                                                                                                         >  1:16102/1‑251 (MQ=255)                                                                                                                        aTGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAatat                                                                                                                                                                                     >  1:301739/1‑186 (MQ=255)                                                                                                                        aTGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTtcct                                                                                                                     >  1:844810/1‑250 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          >  1:438349/1‑135 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCtaatt                                                                                                                                                                                                               >  1:292687/1‑146 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTcc                                                                                                                                                                                          <  1:477608/167‑1 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTcc                                                                                                                                                                                          >  1:648464/1‑167 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCg                                                                                                                                                                <  1:445785/193‑1 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTa                                                                                                                                                         >  1:348099/1‑200 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCAc                                                                                                                                                   >  1:828218/1‑206 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAgg                                                                                                      >  1:443883/1‑251 (MQ=255)                                                                                                                                      gTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAgg                                                                                                      >  1:546782/1‑251 (MQ=255)                                                                                                                                       tATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGt                                                                                                     <  1:674839/251‑1 (MQ=255)                                                                                                                                             tGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGaataa                                                                                                                                          <  1:660412/208‑1 (MQ=255)                                                                                                                                                       ttCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAAc                                                                                                                                                                                                 >  1:277228/1‑143 (MQ=255)                                                                                                                                                               aaTTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTg                                                                              <  1:145233/250‑1 (MQ=255)                                                                                                                                                                        aaCTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGc                                                                    <  1:499188/251‑1 (MQ=255)                                                                                                                                                                               tGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTAc                                                              <  1:67769/250‑1 (MQ=255)                                                                                                                                                                                 cTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTttat                                                                                                                                                                                                                          <  1:290626/92‑1 (MQ=255)                                                                                                                                                                                      gAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTa                                                                                                                                                             <  1:358822/148‑1 (MQ=255)                                                                                                                                                                                             cAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAg                                                                                                                                                                                                         <  1:126132/97‑1 (MQ=255)                                                                                                                                                                                             cAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCAt                                               >  1:218957/1‑251 (MQ=255)                                                                                                                                                                                               gAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGaat                                                                                                                                            <  1:481432/156‑1 (MQ=255)                                                                                                                                                                                                            aaTCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGt                                                                                                                                                      <  1:226449/133‑1 (MQ=255)                                                                                                                                                                                                              tCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGaat                                                                                                                                            <  1:123955/141‑1 (MQ=255)                                                                                                                                                                                                                   gcgcTGATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCt                                                                                                                                                          >  1:746507/1‑122 (MQ=255)                                                                                                                                                                                                                        gATTTTATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCa                     >  1:667326/1‑250 (MQ=255)                                                                                                                                                                                                                          ttttATCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCa                           >  1:539824/1‑242 (MQ=255)                                                                                                                                                                                                                              aTCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAAc                                                                                                                                                                                                 <  1:286829/72‑1 (MQ=255)                                                                                                                                                                                                                              aTCGGTAATCATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAatat                                                                                                                                                                                     >  1:733977/1‑84 (MQ=255)                                                                                                                                                                                                                                       cATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGc                                                                                                                                                                 >  1:583635/1‑95 (MQ=255)                                                                                                                                                                                                                                       cATGAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCgg     >  1:196695/1‑251 (MQ=255)                                                                                                                                                                                                                                          gAAAAGTGCGGTGAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACg  >  1:609010/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ACAACCGACAGCGGTTATTGTCCTTGCGGATATCGCTATTCGAGGCCAAGAACTTGACAGCGAACGCATTCTTGCTGCGAAGAAACGTGCTGAAGAAAACATTCGTCAGCATCACCACGATGCAGATTACGAAGTATTAATGTCCAAACTTTCTAAAGAATTGGCAAAACTTCGTGCTTATGAACTGACAGAACAATTAGTCAAATCTAAGCGCTGATTTTATCGGTAATCATGAAAAGTGCGGTAAAAAACCACCGCACTTTTTTATTATCCCTAATTAATTAGTTTTCAACTTATTCCAATATTTTTCATAAATATCAATGGCGTTACCTACGTCACCTTGAATAATGCCTTTTTTCACTTGTTCCTCCGGCGGGAATAAGGTCGGGTTGTTTGCCATTTCCGGTGAGATTAAGGCTTTTACGCCGTTGTTTGGCATGGAATAGCCCAAGCGTTCCAAAACCACTTTGGCGTTTTCCGGACG  >  1/479861‑480344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑