Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	473,306	G→C	intergenic (‑56484/‑24157)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	473,306	0	G	C	100.0%	228.5 / NA	79	intergenic (‑56484/‑24157)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (44/35);  total (44/35)

TTAATACTTTTGACTAAAAAGTAAAATTTCTGTTAACTATCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCGAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAATAATAT  >  1/473063‑473547                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ttAATACTTTTGACTAAAAAGTAAAATTTCTGTTAACTATCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGc                                                                                                                                                                                                                                            <  1:722681/251‑1 (MQ=255)                      tAAAATTTCTGTTAACTATCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGAtt                                                                                                                                                                                                                       >  1:678357/1‑251 (MQ=255)                             tCTGTTAACTATCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGc                                                                                                                                                                                                                 >  1:841294/1‑250 (MQ=255)                                     cTATCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCAt                                                                                                                                                                                                        >  1:812540/1‑251 (MQ=255)                                       aTCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATa                                                                                                                                                                                                       >  1:872815/1‑250 (MQ=255)                                         cTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTc                                                                                                                                                                                                                                  <  1:100441/221‑1 (MQ=255)                                         cTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATAc                                                                                                                                                                                                      >  1:374124/1‑249 (MQ=255)                                                 gTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTAt                                                                                                                                                                                            >  1:693014/1‑251 (MQ=255)                                                      tCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTtg                                                                                                                                                                                       >  1:831672/1‑251 (MQ=255)                                                       cATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGc                                                                                                                                                                                                                               >  1:660108/1‑210 (MQ=255)                                                                cTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGAt                                                                                                                                                                                                                        >  1:293389/1‑208 (MQ=255)                                                                   tttGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCtttttt                                                                                                                                                                                               <  1:326059/230‑1 (MQ=255)                                                                     tGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGtttttt                                                                                                                                                                        <  1:194646/251‑1 (MQ=255)                                                                           cTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTATGAtttttt                                                                                                                                                                                                                   <  1:667282/202‑1 (MQ=255)                                                                           cTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATc                                                                                                                                                                                           >  1:760227/1‑226 (MQ=255)                                                                           cTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCa                                                                                                                                                                  >  1:762402/1‑251 (MQ=255)                                                                           cTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCa                                                                                                                                                                  >  1:887137/1‑251 (MQ=255)                                                                                  gTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTg                                                                                                                                                                                                                                             >  1:379148/1‑169 (MQ=255)                                                                                        tctcATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATc                                                                                                                                                     <  1:652873/251‑1 (MQ=255)                                                                                                    aaGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAAc                                                                                                                                         <  1:467163/251‑1 (MQ=255)                                                                                                      gggTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTc                                                                                                                                                                                                                                  >  1:657405/1‑160 (MQ=255)                                                                                                      gggTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTAt                                                                                                                                                                      >  1:525887/1‑220 (MQ=255)                                                                                                      gggTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATc                                                                                                                                                     >  1:114510/1‑237 (MQ=255)                                                                                                      gggTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCg                                                                                                                                       >  1:628217/1‑251 (MQ=255)                                                                                                           taATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAg                                                                                                                                                                                                                                               >  1:461744/1‑142 (MQ=255)                                                                                                           taATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATc                                                                                                                                                     >  1:520700/1‑232 (MQ=255)                                                                                                           taATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACtt                                                                                                                                  >  1:556953/1‑251 (MQ=255)                                                                                                              ttGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTAt                                                                                                                               <  1:662533/251‑1 (MQ=255)                                                                                                              ttGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTAt                                                                                                                               <  1:301463/251‑1 (MQ=255)                                                                                                                   ccGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCgtgg                                                                                                                          <  1:388459/251‑1 (MQ=255)                                                                                                                     gTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCgtggtg                                                                                                                        >  1:842867/1‑251 (MQ=255)                                                                                                                      ttttAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCgtggtg                                                                                                                        <  1:591890/250‑1 (MQ=255)                                                                                                                        ttAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAg                                                                                                                     <  1:179143/251‑1 (MQ=255)                                                                                                                             gTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTAt                                                                                                                                                      >  1:14989/1‑213 (MQ=255)                                                                                                                             gTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCAtt                                                                                                                >  1:425900/1‑251 (MQ=255)                                                                                                                             gTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCAtt                                                                                                                >  1:352065/1‑251 (MQ=255)                                                                                                                                       tATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTAt                                                                                                      <  1:839908/251‑1 (MQ=255)                                                                                                                                          gTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGa                                                                                                   >  1:892533/1‑251 (MQ=255)                                                                                                                                          gTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGa                                                                                                   >  1:131625/1‑251 (MQ=255)                                                                                                                                                 gTTTTGGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCg                                                                                                                             >  1:201742/1‑218 (MQ=255)                                                                                                                                                 gTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGAtt                                                                                              >  1:173936/1‑249 (MQ=255)                                                                                                                                                   tttAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTAc                                                                                          <  1:44676/251‑1 (MQ=255)                                                                                                                                                   tttAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTAc                                                                                          <  1:432023/251‑1 (MQ=255)                                                                                                                                                     tAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTg                                                                                        <  1:186568/251‑1 (MQ=255)                                                                                                                                                      aGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATAc                                                                                                                                                                                                      >  1:715909/1‑140 (MQ=255)                                                                                                                                                       gCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTAtgttgt                                                                                                                                                                             >  1:290474/1‑164 (MQ=255)                                                                                                                                                                       aaTATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCaaa                                                                      >  1:586294/1‑251 (MQ=255)                                                                                                                                                                          ataAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATgg                                                                   >  1:44413/1‑251 (MQ=255)                                                                                                                                                                           taAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATgg                                                                   >  1:892167/1‑250 (MQ=255)                                                                                                                                                                                                aTTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTg                                             <  1:838082/251‑1 (MQ=255)                                                                                                                                                                                                           gTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACa                                  >  1:67020/1‑251 (MQ=255)                                                                                                                                                                                                           gTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACa                                  >  1:155844/1‑251 (MQ=255)                                                                                                                                                                                                           gTCTTGTTTTGAGCTCTAGTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACg                                                                                                 <  1:455482/188‑1 (MQ=255)                                                                                                                                                                                                             cTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:159715/251‑1 (MQ=255)                                                                                                                                                                                                             cTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:135074/251‑1 (MQ=255)                                                                                                                                                                                                                gTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGAtt                                                                                              <  1:783975/186‑1 (MQ=255)                                                                                                                                                                                                                        ctctATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGt                                                           <  1:715805/213‑1 (MQ=255)                                                                                                                                                                                                                        ctctATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTg                                             >  1:92138/1‑227 (MQ=255)                                                                                                                                                                                                                          ctATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTTCCGCGTCCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGACTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                >  1:818782/1‑238 (MQ=255)                                                                                                                                                                                                                          ctATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATc                                                                                                                                                                     >  1:317806/1‑105 (MQ=255)                                                                                                                                                                                                                          ctATTATTGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCttt                                     >  1:486143/1‑233 (MQ=255)                                                                                                                                                                                                                            attattGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTAc                                                                                          <  1:6540/178‑1 (MQ=255)                                                                                                                                                                                                                            attattGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGt                                            >  1:370528/1‑224 (MQ=255)                                                                                                                                                                                                                            attattGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTAc                       <  1:659961/245‑1 (MQ=255)                                                                                                                                                                                                                               attGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:379004/233‑1 (MQ=255)                                                                                                                                                                                                                                 tGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGc            <  1:591705/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGc            <  1:119908/251‑1 (MQ=255)                                                                                                                                                                                                                                  gCGTTGTTAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGatt                                                                                     >  1:379039/1‑177 (MQ=255)                                                                                                                                                                                                                                     ttgttAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:495123/227‑1 (MQ=255)                                                                                                                                                                                                                                     ttgttAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:372317/227‑1 (MQ=255)                                                                                                                                                                                                                                     ttgttAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTaaa        >  1:753312/1‑251 (MQ=255)                                                                                                                                                                                                                                      tgttAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAaat       <  1:99141/251‑1 (MQ=255)                                                                                                                                                                                                                                       gttAGCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTAt                                                                                                      >  1:789912/1‑155 (MQ=255)                                                                                                                                                                                                                                           gCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTa                                                >  1:525969/1‑205 (MQ=255)                                                                                                                                                                                                                                           gCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:745455/221‑1 (MQ=255)                                                                                                                                                                                                                                           gCCAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAgg                                <  1:257086/221‑1 (MQ=255)                                                                                                                                                                                                                                            ccAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAATAatat  <  1:606953/250‑1 (MQ=255)                                                                                                                                                                                                                                            ccAATACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTCTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAataata   <  1:436573/249‑1 (MQ=255)                                                                                                                                                                                                                                                tACCCAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGa                                                                                                                                                           <  1:414000/93‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  TTAATACTTTTGACTAAAAAGTAAAATTTCTGTTAACTATCTTTTATTGTAGTTCATTTACCACTTTTTGAGTGCTTTTGAGTTGAATCTCATTTTTTCAAGGGTATAATTGCTCCGTTTTAAGGTTTAAAAGTTATGTCCAGAGTTTTAGCACAAGCCCGAGCCAAATATAAAAAATCCATTTACATTGAATTTTTCGTCGGTCTTGTTTTGAGCTCTATTATTGCGTTGTTAGCCAATACCGAAAGTGCGGTTGATTTCTGCTTAGGATTTTTTGCCGCGTTCATACCTTTTTTTATCTTTGTGTATGTTGTTTTTTATCGCAATCAGGATTTATCAAAAAAATTAACCGCACTTTATCGTGGTGAAGCCATTAAATTCGTATTGACGATTCTACTGATTATTTTTTCGTTCAAATGGCTTTCGGTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAATAATAT  >  1/473063‑473547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑