Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	470,044	A→C	intergenic (‑53222/‑27419)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	470,044	0	A	C	100.0%	215.1 / NA	79	intergenic (‑53222/‑27419)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/48);  total (31/48)

AACGGAATCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATAAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAATTGATGAAGCGCGTTGGGCGCGTTTTAAT  >  1/469805‑470278                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           aaCGGAATCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCggg                                                                                                                                                                                                                                   >  1:144589/1‑249 (MQ=255) aaCGGAATCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATAAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGc                                                                                                                                                                                                                                  <  1:267117/250‑1 (MQ=255)      aaTCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTAtg                                                                                                                                                                                                                            <  1:547958/251‑1 (MQ=255)       aTCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTAtgt                                                                                                                                                                                                                           <  1:580649/251‑1 (MQ=255)       aTCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTAtgt                                                                                                                                                                                                                           <  1:678628/251‑1 (MQ=255)       aTCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGATTGGAAAAGTCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTAtgt                                                                                                                                                                                                                           >  1:102782/1‑251 (MQ=255)              ccaGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGaa                                                                                                                                                                                                                    >  1:540078/1‑251 (MQ=255)                                 gCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTAtg                                                                                                                                                                                                                            >  1:47718/1‑224 (MQ=255)                                  cAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCAcg                                                                                                                                                                                                <  1:867313/251‑1 (MQ=255)                                  cAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCAc                                                                                                                                                                                                 <  1:241107/250‑1 (MQ=255)                                      tGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCAcgtcgt                                                                                                                                                                                            >  1:125305/1‑251 (MQ=255)                                       ggggATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGTCCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGc                                                                                                                                                                                                                         <  1:708509/221‑1 (MQ=255)                                             cGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAgg                                                                                                                                                                                     >  1:154298/1‑251 (MQ=255)                                                    tCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:249414/251‑1 (MQ=255)                                                    tCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:440591/251‑1 (MQ=255)                                                    tCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:440805/251‑1 (MQ=255)                                                    tCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:125403/251‑1 (MQ=255)                                                    tCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:253965/251‑1 (MQ=255)                                                     ccATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATAtt                                                                                                                                                                             >  1:402771/1‑251 (MQ=255)                                                      cATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTAtg                                                                                                                                                                                                                            >  1:179631/1‑203 (MQ=255)                                                       aTGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTgg                                                                                                                                                                           >  1:856018/1‑251 (MQ=255)                                                          aaaGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGgtg                                                                                                                                                                         <  1:859908/250‑1 (MQ=255)                                                          aaaGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGgtg                                                                                                                                                                         <  1:774254/250‑1 (MQ=255)                                                              gTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:894464/241‑1 (MQ=255)                                                                    aaaaGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGga                                                                                                                                                               <  1:602501/250‑1 (MQ=255)                                                                      aaGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGgatga                                                                                                                                                            <  1:293504/251‑1 (MQ=255)                                                                          cACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCtt                                                                                                                                                        <  1:613906/251‑1 (MQ=255)                                                                               aTCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCCCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCAc                                                                                                                                                   <  1:446151/251‑1 (MQ=255)                                                                                tCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCAc                                                                                                                                                   >  1:261297/1‑250 (MQ=255)                                                                                        ccGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTAc                                                                                                                                          <  1:801176/251‑1 (MQ=255)                                                                                          gggTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTg                                                                                                                                              <  1:591455/245‑1 (MQ=255)                                                                                           ggTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGgtg                                                                                                                                                                         <  1:407152/217‑1 (MQ=255)                                                                                               aCGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGaa                                                                                                                                   >  1:396428/1‑251 (MQ=255)                                                                                               aCGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGaa                                                                                                                                   >  1:620158/1‑251 (MQ=255)                                                                                                  ccATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              >  1:850210/1‑205 (MQ=255)                                                                                                  ccATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTatat                                                                                                                                                                              <  1:237964/205‑1 (MQ=255)                                                                                                    aTTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCata                                                                                                                              >  1:857999/1‑251 (MQ=255)                                                                                                       gAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGa                                                                                                                                                                                                               <  1:468708/167‑1 (MQ=255)                                                                                                       gAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGga                                                                                                                                                               >  1:82241/1‑215 (MQ=255)                                                                                                       gAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCatat                                                                                                                             >  1:434006/1‑249 (MQ=255)                                                                                                            cGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCAc                                                                                                                                                   <  1:719275/222‑1 (MQ=255)                                                                                                            cGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAg                                                                                                                                     <  1:842992/236‑1 (MQ=255)                                                                                                                 attTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCt                                                                                                                                                                                   >  1:388017/1‑185 (MQ=255)                                                                                                                 attTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTa                                                                                                                   >  1:328865/1‑249 (MQ=255)                                                                                                                  ttttGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGTGTGTTTACtt                                                                                                                <  1:584862/251‑1 (MQ=255)                                                                                                                        tCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTg                                                                                                          <  1:710116/251‑1 (MQ=255)                                                                                                                                ctctcAAACCGACCTTAGAAACCAACTCGATCTCCGGTTTATTCTTTGCCGGTCACCCTAACGGCACAACCTGTTATGAATAAGCAGCGGCACACTTGCTACTTGCCGCTATCAGCGCCGGGCTTTATCTGCCTTAAAAAGATGCCCGGTTCCCACGTCGCCCTCAGGCTTATAttcg                                                                                                                                                                           >  1:319821/2‑176 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGTGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAatat                                                                                                  <  1:803356/251‑1 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATc                                                                                                                                                                                        >  1:444633/1‑165 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATAtt                                                                                                                                                                             >  1:64911/1‑176 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGgtgtgt                                                                                                                                                                      <  1:254729/183‑1 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCAcc                                                                                                                                                  >  1:803581/1‑203 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTAc                                                                                                                                          <  1:631996/211‑1 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAg                                                                                                                                     >  1:375871/1‑216 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAatat                                                                                                  <  1:240818/251‑1 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAatat                                                                                                  >  1:814228/1‑251 (MQ=255)                                                                                                                                aTCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGACACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGgtg                                                                                                                                                                         <  1:266844/180‑1 (MQ=255)                                                                                                                                 tctcAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGc                                                                                                                                                                                                                                  <  1:157559/122‑1 (MQ=255)                                                                                                                                       aCCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCttgt                                                                                           <  1:83824/251‑1 (MQ=255)                                                                                                                                           aCCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTgttgtt                                                                                       >  1:749298/1‑251 (MQ=255)                                                                                                                                              ttAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGa                                                                                                                                                                                                               >  1:454151/1‑128 (MQ=255)                                                                                                                                              ttAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAg                                                                                                                                     >  1:407103/1‑202 (MQ=255)                                                                                                                                                     ccAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATAtt                                                                                                                                                                             <  1:622188/155‑1 (MQ=255)                                                                                                                                                     ccAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATAtt                                                                                                                                                                             <  1:862294/155‑1 (MQ=255)                                                                                                                                                                        aTTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTAc                                                                                                                                          >  1:845996/1‑171 (MQ=255)                                                                                                                                                                        aTTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTAc                                                                                                                                          >  1:171415/1‑171 (MQ=255)                                                                                                                                                                           cTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGc                                                       <  1:31192/251‑1 (MQ=255)                                                                                                                                                                                         ttAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGaa                                         <  1:898811/251‑1 (MQ=255)                                                                                                                                                                                         ttAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGaa                                         <  1:301455/251‑1 (MQ=255)                                                                                                                                                                                                cacaACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGt                                   <  1:510136/250‑1 (MQ=255)                                                                                                                                                                                                    aCCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTtata                                                                                                                                                                               <  1:699220/106‑1 (MQ=255)                                                                                                                                                                                                    aCCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAAt                              <  1:732330/251‑1 (MQ=255)                                                                                                                                                                                                    aCCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAAt                              >  1:600252/1‑251 (MQ=255)                                                                                                                                                                                                     ccGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAAtt                             <  1:773049/251‑1 (MQ=255)                                                                                                                                                                                                        gTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGAGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCatat                                                                                                                             <  1:243312/152‑1 (MQ=255)                                                                                                                                                                                                                         gCACAAGGTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAATTGATGAAGCGCGTTgctgtc          >  1:417905/1‑245 (MQ=255)                                                                                                                                                                                                                               ggTTTACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTa                                                                                                                                           <  1:412078/115‑1 (MQ=255)                                                                                                                                                                                                                                  ttACTTGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAATTGATGAAGCGCGTTGGGCGCGTTTTAAt  <  1:754907/249‑1 (MQ=255)                                                                                                                                                                                                                                       tGCCGGGATCAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGatat                                                                  <  1:819714/180‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           AACGGAATCTCCACCAGTTTGCCGTTTGATGTGCAAATGGGGATCGTGAATTCCATGAAAGGTTTGGAAAAGGCACGAATCGTCAAACCGGGTTACGCCATTGAATACGATTATTTTGATCCGCGTGATCTCAAACCGACCTTAGAAACCAAAGCGATCTCCGGTTTATTCTTTGCCGGTCAAATTAACGGCACAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTACTTGCCGGGATAAACGCCGGGCTTTATGTGCAGGAAAAAGATGCCTGGTTCCCACGTCGTGATCAGGCTTATATTGGTGTGTTGGTGGATGATCTTTGCACCCTTGGTACTAAAGAACCATATCGCGTGTTTACTTCCCGTGCGGAATATCGCTTGTTGTTACGTGAAGATAACGCCGATATTCGTTTAACGCCGATTGCGCACGAATTAGGTTTAATTGATGAAGCGCGTTGGGCGCGTTTTAAT  >  1/469805‑470278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑