Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	458,613	A→G	intergenic (‑41791/‑38850)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	458,613	0	A	G	100.0%	185.6 / NA	69	intergenic (‑41791/‑38850)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (64/5);  total (64/5)

GTGCAAATTCACATCGGACAAAATCTGTTCCTGCGCATAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCAACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGCACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATGCCACGAATGCTGTCGAGAAAGAAATCGTTCATTT  >  1/458367‑458854                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gtgCAAATTCACATCGGACAAAATCTGTTCCTGCGCATAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCcgacaa                                                                                                                                                                                                                                               >  1:271330/1‑251 (MQ=255)      aaTTCACATCGGACAAAATCTGTTCCTGCGCATAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCa                                                                                                                                                                                                                                          >  1:878418/1‑251 (MQ=255)                      aaTCTGTTCCTGCGCATAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCaa                                                                                                                                                                                                                          >  1:814474/1‑251 (MQ=255)                       aTCTGTTCCTGCGCATAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTACGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATc                                                                                                                                                                                                                                  >  1:442765/1‑242 (MQ=255)                                      tAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATaccgcgagctggtg                                                                                                                                                                                                          >  1:645321/1‑238 (MQ=255)                                            aaaCTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTaa                                                                                                                                                                                                    >  1:780841/1‑251 (MQ=255)                                               ataaaagagacagctttattttGCTCCACCTGGGTGATCCTTCCTCCTTTTCCCCCTTCTTTAAATGCGGGCTGTCCCGCCAGCACCCCAAATCCCCCCCCGCCGCGGGCAGGGGTTTGCAGCGGTTTATGGGAGATATTACTAAAGACAACCACGGGCCCGTAGCCGAAAAAAAAAAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAAt                                                                                                                                                                                                 <  1:102736/232‑1 (MQ=255)                                                 gACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCa                                                                                                                                                                                               >  1:334437/1‑251 (MQ=255)                                                     ttttCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGat                                                                                                                                                                                           >  1:143009/1‑251 (MQ=255)                                                           aCCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTaaa                                                                                                                                                                                     >  1:668408/1‑251 (MQ=255)                                                                               ggTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCg                                                                                                                                                                 >  1:783459/1‑251 (MQ=255)                                                                                  gATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGcc                                                                                                                                                                                                             >  1:522840/1‑204 (MQ=255)                                                                                   aTACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTaaa                                                                                                                                                             >  1:789896/1‑251 (MQ=255)                                                                                    tacttactCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAg                                                                                                                                                            >  1:592713/1‑251 (MQ=255)                                                                                            ccATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACgta                                                                                                                                                     >  1:547478/1‑248 (MQ=255)                                                                                              aTTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTc                                                                                                                                                                   >  1:627798/1‑234 (MQ=255)                                                                                                 ttCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTg                                                                                                                                                >  1:530209/1‑250 (MQ=255)                                                                                                        tCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTc                                                                                                                                           >  1:549694/1‑248 (MQ=255)                                                                                                                        gTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAg                                                                                                                        >  1:424401/1‑251 (MQ=255)                                                                                                                                 gCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGa                                                                                                               >  1:718379/1‑251 (MQ=255)                                                                                                                                     cGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGa                                                                                                           >  1:821687/1‑251 (MQ=255)                                                                                                                                     cGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGa                                                                                                           >  1:73680/1‑251 (MQ=255)                                                                                                                                      gCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAgg                                                                                                                                                           >  1:820793/1‑202 (MQ=255)                                                                                                                                           tACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGAtttttt                                                                                                     >  1:456909/1‑251 (MQ=255)                                                                                                                                                     ccgcTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTg                                                                                           >  1:783501/1‑251 (MQ=255)                                                                                                                                                         tGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGa                                                                                       >  1:35476/1‑251 (MQ=255)                                                                                                                                                                           ggTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAg                                                                                                                        >  1:425545/1‑200 (MQ=255)                                                                                                                                                                           ggTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAAttt                                                                                   >  1:516621/1‑237 (MQ=255)                                                                                                                                                                           ggTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGtttt                                                                     >  1:409986/1‑251 (MQ=255)                                                                                                                                                                            gTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGttttt                                                                    >  1:657965/1‑251 (MQ=255)                                                                                                                                                                             ttATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATTACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTg                                                                   >  1:861069/1‑251 (MQ=255)                                                                                                                                                                             ttATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACgtac                                                                                                                                                    >  1:664295/1‑167 (MQ=255)                                                                                                                                                                             ttATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTg                                                                   >  1:840190/1‑251 (MQ=255)                                                                                                                                                                             ttATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTg                                                                   >  1:110726/1‑251 (MQ=255)                                                                                                                                                                               aTTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGtt                                                                 >  1:69251/1‑251 (MQ=255)                                                                                                                                                                               aTTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATACCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGtt                                                                 >  1:475066/1‑251 (MQ=255)                                                                                                                                                                                  ggAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGt                                                              >  1:206353/1‑251 (MQ=255)                                                                                                                                                                                    agagaTTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTc                                                                >  1:191163/1‑247 (MQ=255)                                                                                                                                                                                      agaTTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGc                                                           >  1:158925/1‑250 (MQ=255)                                                                                                                                                                                          tACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAAt                                                      >  1:821844/1‑251 (MQ=255)                                                                                                                                                                                           aCTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCtgttg                                                                                                                                     >  1:421987/1‑171 (MQ=255)                                                                                                                                                                                           aCTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAg                                                                                                                        >  1:667142/1‑184 (MQ=255)                                                                                                                                                                                           aCTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATa                                                     >  1:602724/1‑251 (MQ=255)                                                                                                                                                                                           aCTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATa                                                     >  1:468144/1‑251 (MQ=255)                                                                                                                                                                                           aCTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATa                                                     >  1:228408/1‑251 (MQ=255)                                                                                                                                                                                             taaaaacaacccccGGTCCGTAGCGGAACAAAAGAAAAACCCCCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAAc                                                   <  1:334923/239‑1 (MQ=255)                                                                                                                                                                                                 ggCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTc                                                                >  1:244345/1‑234 (MQ=255)                                                                                                                                                                                                 ggCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGaa                                               >  1:712580/1‑251 (MQ=255)                                                                                                                                                                                                 ggCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGaa                                               >  1:258399/1‑251 (MQ=255)                                                                                                                                                                                                       cACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAAtttctt                                         >  1:458287/1‑251 (MQ=255)                                                                                                                                                                                                         ccGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAAtttcttt                                        >  1:632946/1‑250 (MQ=255)                                                                                                                                                                                                           ggTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAAtt                                                                                                                                                                                                                                       >  1:618054/1‑57 (MQ=255)                                                                                                                                                                                                            gTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTaaaa                                                                                                                                                                                                  >  1:824342/1‑93 (MQ=255)                                                                                                                                                                                                            gTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGaa                                                                                      >  1:22971/1‑201 (MQ=255)                                                                                                                                                                                                            gTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCAta                                    >  1:569254/1‑250 (MQ=255)                                                                                                                                                                                                            gTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCAta                                    >  1:195803/1‑250 (MQ=255)                                                                                                                                                                                                            gTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCAta                                    >  1:153163/1‑250 (MQ=255)                                                                                                                                                                                                             ttgaaagcgaaaaaaaaaaaaaaCCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCAtac                                   <  1:271791/232‑3 (MQ=255)                                                                                                                                                                                                                   gCCGGACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGaa                             >  1:719422/1‑251 (MQ=255)                                                                                                                                                                                                                       gACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCAtac                                   >  1:384453/1‑239 (MQ=255)                                                                                                                                                                                                                       gACATAAGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATg                           >  1:842214/1‑249 (MQ=255)                                                                                                                                                                                                                       aaaaaaaaaaaaaCCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGc                          <  1:813521/241‑1 (MQ=255)                                                                                                                                                                                                                             aGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCgag                   >  1:524936/1‑251 (MQ=255)                                                                                                                                                                                                                             aGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCgag                   >  1:697239/1‑251 (MQ=255)                                                                                                                                                                                                                             aGCAAAATCACCGCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCgag                   >  1:19108/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCCACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAAttt                                                                                   >  1:792797/1‑175 (MQ=255)                                                                                                                                                                                                                                          ccACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCGAGAAAGAAATCGtt       >  1:456702/1‑250 (MQ=255)                                                                                                                                                                                                                                          ccACTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCGAGAAAGAAATCGtt       >  1:613813/1‑250 (MQ=255)                                                                                                                                                                                                                                             cTAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCGAGAAAGAAATCGTTCAtt   >  1:367187/1‑251 (MQ=255)                                                                                                                                                                                                                                              tAACAAGCCGACAAAATCAATTTTATCATAAGCAAATAACGCTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGTACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATACCACGAATGCTGTCGAGAAAGAAATCGTTCAttt  <  1:885286/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GTGCAAATTCACATCGGACAAAATCTGTTCCTGCGCATAAGCAAAACTGACATTTTCTACCTTAATTTGCTGCACATCGGTGATACTTACTCCATTTTCCACGTCTTTCAATTCAGGCTGTTCCGCCAGCAACGCCAATACCCGCTCGCCGCTGGCAAGGGTTTGCAGCAGGTTATTGGAGAGATTACTTAAGGCAATCACCGGTCCGTAGCCGGACATAAGCAAAATCACCGCCACTAACAAGCCAACAAAATCAATTTTATCATAAGCAAATAACGTTAAGCCCACCAGTAAAATCACGATATTAAAAAACGATACGGCGATTTCCGTAAAGGAACGCACATTGCCTTCCTGTTGTTTGATTTTTAAGAATGCCTGATCGATTTTTTCGCTGCGTTGGTGAATTTCCTGTAGGCGTTTTTGTTCGTTGCCGAATAACTGAATTTCTTTCATGCCACGAATGCTGTCGAGAAAGAAATCGTTCATTT  >  1/458367‑458854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑