Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	446,860	C→T	intergenic (‑30038/‑50603)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	446,860	0	C	T	100.0%	175.6 / NA	66	intergenic (‑30038/‑50603)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/36);  total (30/36)

AATTTACCGTATATTTCAATGATCTTATCTAAAAACAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAACCCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTCGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACATTTTGTTCCTTAATTGCCACAC  >  1/446614‑447101                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  aaTTTACCGTATATTTCAATGATCTTATCTAAAAACAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAg                                                                                                                                                                                                                                               <  1:643353/251‑1 (MQ=255)         gTATATTTCAATGATCTTATCTAAAAACAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTg                                                                                                                                                                                                                                       >  1:62422/1‑251 (MQ=255)          tataTTTCAATGATCTTATCTAAAAACAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTgg                                                                                                                                                                                                                                      <  1:175375/251‑1 (MQ=255)                tCAATGATCTTATCTAAAAACAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTaa                                                                                                                                                                                                                                >  1:376920/1‑251 (MQ=255)                               aaaaaCAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTtctc                                                                                                                                                                                                                  >  1:548775/1‑250 (MQ=255)                                               cTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGcc                                                                                                                                                                                                       >  1:317538/1‑245 (MQ=255)                                                           ctttAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCaatg                                                                                                                                                                                              <  1:277561/242‑1 (MQ=255)                                                                   atTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGc                                                                                                                                                                             <  1:637468/251‑1 (MQ=255)                                                                         tGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGa                                                                                                                                                                       <  1:195772/251‑1 (MQ=255)                                                                          gTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAg                                                                                                                                                                        <  1:412841/249‑1 (MQ=255)                                                                           tttCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATg                                                                                                                                                                     <  1:550887/251‑1 (MQ=255)                                                                              cccccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGt                                                                                                                                                                                       >  1:252636/1‑230 (MQ=255)                                                                              cccccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGaa                                                                                                                                                                   <  1:754726/250‑1 (MQ=255)                                                                                 ccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGTCTTGAACTCATGGTAAATTTCCGGCAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATACACCAAGATTGCGTATAAAATATTAATTTTTGTGGTTTCATAGGAGTTAAATTAGAAATTTCTCCAATATTAGTCTTGTCAATGAATGAGTTGGCGATAGCAATATATg                                                                                                                                                                     >  1:423181/1‑245 (MQ=255)                                                                                 ccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAg                                                                                                                                                                        <  1:792934/242‑1 (MQ=255)                                                                                 ccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATg                                                                                                                                                                     <  1:122176/245‑1 (MQ=255)                                                                                 ccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATaa                                                                                                                                                                >  1:154912/1‑250 (MQ=255)                                                                                 ccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATaa                                                                                                                                                                >  1:363706/1‑250 (MQ=255)                                                                                 ccATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAACGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATaa                                                                                                                                                                >  1:863150/1‑250 (MQ=255)                                                                                       atCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACaa                                                                                                                                                         >  1:866052/1‑251 (MQ=255)                                                                                        tCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAAt                                                                                                                                                        <  1:108876/251‑1 (MQ=255)                                                                                           gTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATg                                                                                                                                                                     <  1:32345/235‑1 (MQ=255)                                                                                           gTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGAAGTTAAATTAGAAATTTCTCCAATATTAGcc                                                                                                                                                                                                       >  1:443488/1‑201 (MQ=255)                                                                                                 aaaCTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTAcctc                                                                                                                                                 >  1:612945/1‑249 (MQ=255)                                                                                                   aCTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGc                                                                                                                                                                             >  1:885377/1‑219 (MQ=255)                                                                                                   aCTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGAACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTg                                                                                                                                                                                                                                       >  1:860371/1‑161 (MQ=255)                                                                                                      agagATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTAtt                                                                                                                                           >  1:161934/1‑250 (MQ=255)                                                                                                            aaTTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTataat                                                                                                                                    <  1:649931/251‑1 (MQ=255)                                                                                                                     aCCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTataa                                                                                                                                     >  1:641096/1‑241 (MQ=255)                                                                                                                                  aaCTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGAt                                                                                                              <  1:639653/251‑1 (MQ=255)                                                                                                                                    cTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAAccc                                                                                                                                                             >  1:815136/1‑202 (MQ=255)                                                                                                                                      cATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGtt                                                                                                                                                                                      >  1:273822/1‑175 (MQ=255)                                                                                                                                       aTGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGtat                                                                                                         <  1:373883/251‑1 (MQ=255)                                                                                                                                          gTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATAc                                                                                                       <  1:604731/250‑1 (MQ=255)                                                                                                                                            aaaTTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCtt                                                                                                    <  1:147462/251‑1 (MQ=255)                                                                                                                                                  ccGGTAGCACCGGACCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTg                                                                                              <  1:250505/251‑1 (MQ=255)                                                                                                                                                               aCCAAATTGCCATCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTataat                                                                                                                                    <  1:777480/200‑1 (MQ=255)                                                                                                                                                                           tCTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAAt                                                                                                                                                        <  1:170472/168‑1 (MQ=255)                                                                                                                                                                            cTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAAc                                                                                      >  1:212788/1‑233 (MQ=255)                                                                                                                                                                            cTTTCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGt                                                                             >  1:96971/1‑242 (MQ=255)                                                                                                                                                                              ttCAAACCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAAt                                                                                                                                                        >  1:723637/1‑165 (MQ=255)                                                                                                                                                                                        cccTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATAc                                                                                <  1:824991/227‑1 (MQ=255)                                                                                                                                                                                              aacaaaGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCt                                                                                                                                         <  1:794367/164‑1 (MQ=255)                                                                                                                                                                                              aacaaaGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCtt                                                                                                    >  1:199219/1‑201 (MQ=255)                                                                                                                                                                                              aacaaaGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATAc                                                                                <  1:632619/221‑1 (MQ=255)                                                                                                                                                                                                    ggCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTAcctcc                                                                                                                                                <  1:396285/151‑1 (MQ=255)                                                                                                                                                                                                    ggCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGAt                                                                                                              <  1:41579/185‑1 (MQ=255)                                                                                                                                                                                                      cTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAAAGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAAAACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATac                                          <  1:257416/251‑1 (MQ=255)                                                                                                                                                                                                          ccATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCt                                                                                                                                         >  1:795652/1‑152 (MQ=255)                                                                                                                                                                                                          ccATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCa                                      <  1:358984/251‑1 (MQ=255)                                                                                                                                                                                                              aaaGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATAcc                                                                                                      >  1:193168/1‑183 (MQ=255)                                                                                                                                                                                                              aaaGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGtat                                                                           <  1:667228/210‑1 (MQ=255)                                                                                                                                                                                                                 gCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAAt                                                                                                                                                        >  1:702595/1‑130 (MQ=255)                                                                                                                                                                                                                 gCTTAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTAt                               >  1:266050/1‑251 (MQ=255)                                                                                                                                                                                                                   ttAATATACCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCt                             <  1:652115/251‑1 (MQ=255)                                                                                                                                                                                                                         taCCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAg                                                                                                                                                                                                                                    <  1:591418/46‑1 (MQ=255)                                                                                                                                                                                                                         taCCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATAcc                                                                                                      <  1:820268/172‑1 (MQ=255)                                                                                                                                                                                                                         taCCAAGATTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATacac                                        >  1:640393/1‑234 (MQ=255)                                                                                                                                                                                                                                aTTGCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACATTTTGTTc                <  1:510052/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCGTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACATTTTGTTCCtt             >  1:616027/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTATAAAACATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCAttt                                                                 <  1:611873/197‑1 (MQ=255)                                                                                                                                                                                                                                            aCATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATAc                                                                                >  1:836847/1‑175 (MQ=255)                                                                                                                                                                                                                                            aCATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATAc                                                                                <  1:771359/175‑1 (MQ=255)                                                                                                                                                                                                                                            aCATTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACAt                       >  1:581967/1‑232 (MQ=255)                                                                                                                                                                                                                                              aTTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACATTTTGTTCCTTAATTGCCacac  <  1:78109/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTTAATTTTTGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACATTTTGTTCCTTAATTGCCacac  <  1:520584/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  AATTTACCGTATATTTCAATGATCTTATCTAAAAACAACAGAGTATCTTTATCACTTTCTTTAATAATTTGCTGTTTCCCCCATATATCAGTACCAAAACTAGAGATAATTCGAGAACCAAATGGTTTGAACTCATGGTAAATTTCCGGTAGCACCGGACCAAATTGCCATCTTTCAACCCCCCCCTCAAACAAAGGCTTACCATAAAGCTTAATATACCAAGATTGCGTATAAAACATTAATTTTCGTAGTTTCATTGGAGTTAAATTAGAAATTTCTCCAATATTAGCCTTGCCAATGAATGAGTTGGCGATAGCAATAGATGAATAACCCACAATTTACCTCCTTATTCTATAATAAACTAAAATTTGGCTTAGGATTGTATACCTTTCTCTGTTATAAACCAATACTGTATACCACCATTTTCTTTGTAAATTTGAGTAAATACACCATGCGTATCTTCACATTTTGTTCCTTAATTGCCACAC  >  1/446614‑447101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑