Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	445,701	A→C	intergenic (‑28879/‑51762)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	445,701	0	A	C	100.0%	94.0 / NA	33	intergenic (‑28879/‑51762)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/22);  total (11/22)

CTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGGGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAAATCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAACTGAGTTCTAATTTGCAACTCTAATT  >  1/445506‑445924                                                                                                                                                                                                    |                                                                                                                                                                                                                                cTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCtatta                                                                                                                                                                          <  1:148895/251‑1 (MQ=255)                          gAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAatta                                                                                                                                                                                          <  1:583978/210‑1 (MQ=255)                               taCCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCacaac                                                                                                                                                                                                    <  1:58929/195‑1 (MQ=255)                                            ttAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAAttt                                                                                                                               <  1:491089/251‑1 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCacaac                                                                                                                                                                                                    <  1:760546/114‑1 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGa                                                                                                                                                                     <  1:94131/145‑1 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAg                                                                                                                                                                    <  1:159244/146‑1 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAaataat                                                                                                                                             <  1:512116/169‑1 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTAc                                                                                                                                      >  1:278819/1‑176 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAAttt                                                                                                                               >  1:807560/1‑183 (MQ=255)                                                                                                                ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAata                                                           >  1:417914/1‑251 (MQ=255)                                                                                                                 taataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAatat                                                          <  1:662483/251‑1 (MQ=255)                                                                                                                          gcgcTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAAttt                                                                                                                               <  1:856748/173‑1 (MQ=255)                                                                                                                          gcgcTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTc                                                       <  1:790113/245‑1 (MQ=255)                                                                                                                             cTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATgg                                               <  1:397539/250‑1 (MQ=255)                                                                                                                             cTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATgg                                               <  1:552175/250‑1 (MQ=255)                                                                                                                               agagTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCtatta                                                                                                                                                                          <  1:565176/122‑1 (MQ=255)                                                                                                                                  gTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCAcc                                                                                                    >  1:635820/1‑192 (MQ=255)                                                                                                                                   ttATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGccc                                         <  1:842812/250‑1 (MQ=255)                                                                                                                                       gTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCa                                    >  1:367770/1‑251 (MQ=255)                                                                                                                                        ttCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCtat                                                                                                                                                                            >  1:154492/1‑114 (MQ=255)                                                                                                                                            aCATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCAtgttg                               <  1:182696/251‑1 (MQ=255)                                                                                                                                            aCATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCAtgt                                 >  1:267522/1‑249 (MQ=255)                                                                                                                                                cAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAaat                                                                                                                        <  1:772825/158‑1 (MQ=255)                                                                                                                                                cAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTaa                            >  1:65236/1‑250 (MQ=255)                                                                                                                                                cAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAAc                           <  1:382253/251‑1 (MQ=255)                                                                                                                                                cAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAAc                           <  1:321221/251‑1 (MQ=255)                                                                                                                                                      cACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAACTg                         >  1:530787/1‑247 (MQ=255)                                                                                                                                                            cGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAACTGAGt                      >  1:641728/1‑244 (MQ=255)                                                                                                                                                                         aTCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTgg                                                                                                                                                                      <  1:874222/87‑1 (MQ=255)                                                                                                                                                                         aTCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAACTGAGTTCTAATTTGCAACTCTAAtt  >  1:631028/1‑251 (MQ=255)                                                                                                                                                                         aTCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAACTGAGTTCTAATTTGCAACTCTAAtt  <  1:738530/251‑1 (MQ=255)                                                                                                                                                                           cTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAAc                           <  1:69353/224‑1 (MQ=255)                                                                                                                                                                                                    |                                                                                                                                                                                                                                CTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGGGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAAATCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGGTTGCCCACTCATGTTGTAACTGAGTTCTAATTTGCAACTCTAATT  >  1/445506‑445924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑