Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	445,634	G→A	intergenic (‑28812/‑51829)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	445,634	0	G	A	100.0%	79.0 / NA	30	intergenic (‑28812/‑51829)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/20);  total (10/20)

TAAGTTTTCCATTCTATTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGGGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAAATCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGG  >  1/445392‑445879                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      tAAGTTTTCCATTCTATTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATg                                                                                                                                                                                                                                                 <  1:483265/249‑1 (MQ=255)    gTTTTCCATTCTATTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTa                                                                                                                                                                                                                                                   >  1:522371/1‑244 (MQ=255)    gTTTTCCATTCTATTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTc                                                                                                                                                                                                                                              <  1:727364/249‑1 (MQ=255)       ttCCATTCTATTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACAt                                                                                                                                                                                                                                         <  1:472192/251‑1 (MQ=255)                aTTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCAc                                                                                                                                                                                                                                <  1:414681/251‑1 (MQ=255)                          cTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCtt                                                                                                                                                                                                                        <  1:54695/249‑1 (MQ=255)                                           ataatTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTacac                                                                                                                                                                                                     <  1:668547/251‑1 (MQ=255)                                                  gggTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCAc                                                                                                                                                                                                                                >  1:416588/1‑217 (MQ=255)                                                  gggTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAAttt                                                                                                                                                                                              >  1:287361/1‑251 (MQ=255)                                                              tttAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCt                                                                                                                                                                                                   >  1:65738/1‑234 (MQ=255)                                                                                                                   cTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCtatta                                                                                                                             <  1:148895/251‑1 (MQ=255)                                                                                                                                            gAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTac                                                                                                                                                                                                       >  1:644944/1‑152 (MQ=255)                                                                                                                                            gAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAatta                                                                                                                                             <  1:583978/210‑1 (MQ=255)                                                                                                                                                 taCCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCacaac                                                                                                                                                       <  1:58929/195‑1 (MQ=255)                                                                                                                                                              ttAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAAttt                                                                                  <  1:491089/251‑1 (MQ=255)                                                                                                                                                                                                       atGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAAt                                                                                                                                                                                                >  1:84300/1‑100 (MQ=255)                                                                                                                                                                                                                                 aataatATGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTacac                                                                                                                                                                                                     >  1:531478/1‑69 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCacaac                                                                                                                                                       <  1:760546/114‑1 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGa                                                                                                                        <  1:94131/145‑1 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAg                                                                                                                       <  1:159244/146‑1 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAaataat                                                                                                <  1:512116/169‑1 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTAc                                                                                         >  1:278819/1‑176 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAAttt                                                                                  >  1:807560/1‑183 (MQ=255)                                                                                                                                                                                                                                  ataataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAata              >  1:417914/1‑251 (MQ=255)                                                                                                                                                                                                                                   taataTGCTGCGCTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAatat             <  1:662483/251‑1 (MQ=255)                                                                                                                                                                                                                                            gcgcTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAAt                                                                                                                                                                                         <  1:472386/70‑1 (MQ=255)                                                                                                                                                                                                                                            gcgcTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAAttt                                                                                  <  1:856748/173‑1 (MQ=255)                                                                                                                                                                                                                                            gcgcTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTc          <  1:790113/245‑1 (MQ=255)                                                                                                                                                                                                                                               cTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATgg  <  1:552175/250‑1 (MQ=255)                                                                                                                                                                                                                                               cTAGAGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAACTCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATgg  <  1:397539/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      TAAGTTTTCCATTCTATTTATGAAGCTAGTTGTATCTAAGAAATAATTAGGGTACGCATCTTTTAATTGGTTTACATCATCAACCGAAACTAGCACCACATCAAGAAATTCATTCTTAAAAGAATCTTGTTCATAATGGGAGTATACCTGCTCAGCTTTAAATTTCTCATTCTCATTAAAAGTTTTAATTTCAGTATTATGTTTTTTTAAATCTAATATTAACAAATAATATGCTGCGCTAGGGTTATGTTCTACATCAATAGCACTAACGCTTTTTAGTTTATCTATTACACCTAATTTTGAATCTAAATCTTTTATTTTTTCACATATTTGCACAACAGAAAAATTATTAAACTCTTCTATTATTGGAGTATCTTCCTGATAAGAAAATAATGCACTACATAATTTCAAAAATAATCTTATTCCCTCATCACCTTGTCCAACTTTAAATGATGTTTTCATCAAAGAGCCCAATATTTCAACTATGG  >  1/445392‑445879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑