Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	429,058	C→T	intergenic (‑12236/‑68405)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	429,058	0	C	T	100.0%	195.3 / NA	72	intergenic (‑12236/‑68405)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/40);  total (32/40)

AAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAATCCAAAGCAAACTTATCAAATCCCAAAAGCCCTTGAAAATATTAACCTCAAGGGCTTTTTTATATCCTAAGCGTTCCTACCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTGCTCATGTTTTAGCTATATCCAAATTTTTAAAAGTCTGAATCTCCGTCGGAGTTATTTAGAACAGTTTTCAACTCTCAGAAAGAAGCAATATGGCACGCACAATCACGCCGTTAAACAGCACGAAAATAGACAAA  >  1/428812‑429302                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aaGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAAt                                                                                                                                                                                                                                                 <  1:796451/251‑1 (MQ=255)        cGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATaa                                                                                                                                                                                                                                          <  1:107541/251‑1 (MQ=255)         gTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATACCAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATaa                                                                                                                                                                                                                                          <  1:485932/250‑1 (MQ=255)          tttGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATaaaa                                                                                                                                                                                                                                        <  1:401771/251‑1 (MQ=255)          tttGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATaaaa                                                                                                                                                                                                                                        <  1:702802/251‑1 (MQ=255)            tGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATc                                                                                                                                                                                                                                      <  1:703439/251‑1 (MQ=255)                        cATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAg                                                                                                                                                                                                                          <  1:450154/251‑1 (MQ=255)                             aattaaatCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATaa                                                                                                                                                                                                                                          >  1:267982/1‑230 (MQ=255)                                  aatCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGttt                                                                                                                                                                                                                >  1:547422/1‑251 (MQ=255)                                     cAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGt                                                                                                                                                                                                             <  1:583234/251‑1 (MQ=255)                                          tAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATAc                                                                                                                                                                                                        <  1:593805/251‑1 (MQ=255)                                          tAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATAc                                                                                                                                                                                                        <  1:479703/251‑1 (MQ=255)                                           aGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATcc                                                                                                                                                                                                                                               >  1:539839/1‑211 (MQ=255)                                            gCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTta                                                                                                                                                                                                                       <  1:448242/234‑1 (MQ=255)                                               tAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATcc                                                                                                                                                                                                                                               >  1:333447/1‑207 (MQ=255)                                               tAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGAtc                                                                                                                                                                                                   <  1:741175/251‑2 (MQ=255)                                                 aTTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGc                                                                                                                                                                                                 <  1:741132/251‑1 (MQ=255)                                                     tCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACg                                                                                                                                                                                                                   >  1:885298/1‑229 (MQ=255)                                                         gtgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATAc                                                                                                                                                                                                        >  1:257305/1‑236 (MQ=255)                                                         gtgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTg                                                                                                                                                                                                      >  1:159848/1‑238 (MQ=255)                                                          tgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATcc                                                                                                                                                                                                                                               <  1:311278/196‑1 (MQ=255)                                                                      ggATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTg                                                                                                                                                                                                      >  1:46508/1‑225 (MQ=255)                                                                       gATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATaaaa                                                                                                                                                                                                                                        <  1:11837/190‑1 (MQ=255)                                                                         ttAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTa                                                                                                                                                                         <  1:151850/251‑1 (MQ=255)                                                                                  tttAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAAc                                                                                                                                                                     <  1:575117/246‑1 (MQ=255)                                                                                             gACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATAc                                                                                                                                                                                                        >  1:98202/1‑200 (MQ=255)                                                                                             gACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTa                                                                                                                                                     >  1:661077/1‑251 (MQ=255)                                                                                                  aaCGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATcc                                                                                                                                                                                                                                               <  1:234182/156‑1 (MQ=255)                                                                                                  aaCGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGCATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAAc                                                                                                                                                                     >  1:441661/1‑230 (MQ=255)                                                                                                     gtgtAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATAc                                                                                                                                                                                                        <  1:3565/192‑1 (MQ=255)                                                                                                     gtgtAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAAc                                                                                                                                                                                         <  1:229102/207‑1 (MQ=255)                                                                                                            gCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGtttta                                                                                                                                        >  1:769361/1‑248 (MQ=255)                                                                                                            gCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGtttta                                                                                                                                        >  1:359510/1‑248 (MQ=255)                                                                                                                         gAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGc                                                                                                                           <  1:598667/249‑1 (MQ=255)                                                                                                                            tttCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCtatac                                                                                                                      <  1:388179/251‑2 (MQ=255)                                                                                                                             ttCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCtatacc                                                                                                                     <  1:675331/251‑3 (MQ=255)                                                                                                                                     ggggTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCtatacctgattttt                                                                                                             >  1:186856/1‑241 (MQ=255)                                                                                                                                     ggggTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCtatacctgattttt                                                                                                             >  1:453150/1‑241 (MQ=255)                                                                                                                                        gTTCAACTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTg                                                                                                                                                                                                      <  1:804521/159‑1 (MQ=255)                                                                                                                                             aCTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACt                                                                                                                                                                          >  1:851032/1‑182 (MQ=255)                                                                                                                                             aCTCCCCCCAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTc                                                                                                     <  1:354671/251‑1 (MQ=255)                                                                                                                                                ccccccAGCTCCACCAAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAg                                                                                                                                                                                                                          <  1:868308/131‑1 (MQ=255)                                                                                                                                                          ccaccaAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTa                                                                                                                                                                         >  1:47129/1‑170 (MQ=255)                                                                                                                                                             ccaAATAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAAttgccgtcagag                                                                                     <  1:303433/251‑12 (MQ=255)                                                                                                                                                                   aaCAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTAttc                                                                               <  1:511587/251‑2 (MQ=255)                                                                                                                                                                        accAAAGCAAACTTATCAAATCCTAAAGGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGaat                                                                          <  1:474528/250‑2 (MQ=255)                                                                                                                                                                                      aTCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGaataattt                                                                     >  1:330165/1‑236 (MQ=255)                                                                                                                                                                                           aTCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCagaac                                                       <  1:482360/251‑2 (MQ=255)                                                                                                                                                                                            tcctaaaaGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCagaa                                                        >  1:19613/5‑249 (MQ=255)                                                                                                                                                                                                aaaaGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCagaactaagc                                                  <  1:327307/251‑7 (MQ=255)                                                                                                                                                                                                     cccTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCagaac                                                       >  1:446983/1‑240 (MQ=255)                                                                                                                                                                                                      ccTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATg                                            >  1:1553/1‑251 (MQ=255)                                                                                                                                                                                                      ccTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATg                                            >  1:845765/1‑251 (MQ=255)                                                                                                                                                                                                              gtatTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCt                                                                                                                          >  1:881967/2‑165 (MQ=255)                                                                                                                                                                                                              gtatTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGt                                                                                    >  1:369005/2‑203 (MQ=255)                                                                                                                                                                                                                atTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACaa                                  >  1:147236/1‑251 (MQ=255)                                                                                                                                                                                                                     ctttaaGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTg                                                                                                                                                        <  1:874602/124‑1 (MQ=255)                                                                                                                                                                                                                      tttaaGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCAcac                            <  1:715331/248‑3 (MQ=255)                                                                                                                                                                                                                                 ttttATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAg                  >  1:610742/1‑251 (MQ=255)                                                                                                                                                                                                                                  tttATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAAtt                                                                                                                                                                                                                              <  1:3409/46‑1 (MQ=255)                                                                                                                                                                                                                                  tttATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAg                  >  1:252627/1‑250 (MQ=255)                                                                                                                                                                                                                                     atatCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAAc                                                                                                                                                                           <  1:550464/94‑1 (MQ=255)                                                                                                                                                                                                                                       atCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTg                                                                                                                                                        <  1:878619/111‑1 (MQ=255)                                                                                                                                                                                                                                        tCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAGCACGaaa           >  1:489121/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAGCACGAAAATAGAc     <  1:348164/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATAc                                                                                                                                                                                                        >  1:260760/1‑53 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTc                                                                                                                                     <  1:216784/120‑1 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCtatacc                                                                                                                     <  1:7605/136‑3 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCagaac                                                       <  1:229792/198‑2 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAGCACGAAAATAGACaaa  >  1:787109/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAGCACGAAAATAGACaaa  >  1:511437/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTACTCATGTTTTAGCTATACCTGATTTTTTAAAAGTCTGAATTGCCGTCAGAGTTATTCAGAATAATTTTCAACTCTCAGAACTAAGCGATATGGCACGCACAATCACACCGCTAAACAGCACGAAAATAGACaaa  >  1:629674/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAACAATCCAAAGCAAACTTATCAAATCCCAAAAGCCCTTGAAAATATTAACCTCAAGGGCTTTTTTATATCCTAAGCGTTCCTACCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTAGTTATACTGATAGCCGTATAACAAAATCGGTATAACTAAAACCATACAAAACCTGTTAGCAAAAGGTTTTGCTCATGTTTTAGCTATATCCAAATTTTTAAAAGTCTGAATCTCCGTCGGAGTTATTTAGAACAGTTTTCAACTCTCAGAAAGAAGCAATATGGCACGCACAATCACGCCGTTAAACAGCACGAAAATAGACAAA  >  1/428812‑429302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑