Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,098,929	T→C	intergenic (‑43046/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,098,929	0	T	C	100.0%	184.1 / NA	66	intergenic (‑43046/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/32);  total (34/32)

ATCCGCCGGTGTCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGATGGCAACCGAAAAAACCGCGATGGGCGGATAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCACGGTTAACACCGAATTCAGCA  >  1/2098694‑2099169                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 atCCGCCGGTGTCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAAc                                                                                                                                                                                                                                   <  1:103296/251‑1 (MQ=255) atCCGCCGGTGTCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAAc                                                                                                                                                                                                                                   <  1:311240/251‑1 (MQ=255) atCCGCCGGTGTCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAAc                                                                                                                                                                                                                                   <  1:214442/251‑1 (MQ=255)        ggTGTCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATg                                                                                                                                                                                                                            >  1:509907/1‑251 (MQ=255)         gtgtCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATgg                                                                                                                                                                                                                           >  1:711196/1‑251 (MQ=255)                      gcAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCg                                                                                                                                                                                                              >  1:130504/1‑251 (MQ=255)                          aTGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAgg                                                                                                                                                                                                          >  1:500470/1‑251 (MQ=255)                          aTGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAgg                                                                                                                                                                                                          <  1:788724/251‑1 (MQ=255)                           tGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGa                                                                                                                                                                                                         <  1:766995/251‑1 (MQ=255)                               gATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCa                                                                                                                                                                                                     >  1:875222/1‑251 (MQ=255)                                  tAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACgg                                                                                                                                                                                                  <  1:535633/251‑1 (MQ=255)                                  tAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACgg                                                                                                                                                                                                  <  1:637730/251‑1 (MQ=255)                                    aTTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAAc                                                                                                                                                                                                                                   <  1:421258/216‑1 (MQ=255)                                    aTTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGAt                                                                                                                                                                                                >  1:892889/1‑251 (MQ=255)                                     ttgttgGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATg                                                                                                                                                                                               >  1:877088/1‑251 (MQ=255)                                        ttgGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTc                                                                                                                                                                                             <  1:90623/250‑1 (MQ=255)                                            cTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCAc                                                                                                                                                                                        <  1:688002/251‑1 (MQ=255)                                                    cAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGtt                                                                                                                                                                                <  1:493058/251‑1 (MQ=255)                                                       aaaGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGttttt                                                                                                                                                                             >  1:250251/1‑251 (MQ=255)                                                                     aTTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGAAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAAc                                                                                                                                                               <  1:852910/251‑1 (MQ=255)                                                                                ttttCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCtttttt                                                                                                                                                    >  1:360526/1‑251 (MQ=255)                                                                                          cTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGa                                                                                                                                          >  1:387781/1‑251 (MQ=255)                                                                                           tGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGAc                                                                                                                                         <  1:406438/251‑1 (MQ=255)                                                                                                     aaaTCTAAGGTTTTGGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACaa                                                                                                                               <  1:643354/251‑1 (MQ=255)                                                                                                                          gaAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGCTAATTGCCAGATAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCg                                                                                                          >  1:622994/1‑251 (MQ=255)                                                                                                                           aaaTAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGa                                                                                                                                                                                                             >  1:632940/1‑151 (MQ=255)                                                                                                                           aaaTAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACaa                                                                                                                               >  1:309714/1‑229 (MQ=255)                                                                                                                             ataatCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTtg                                                                                                       <  1:89204/251‑1 (MQ=255)                                                                                                                                          tAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGtt                                                                                          >  1:162754/1‑251 (MQ=255)                                                                                                                                                ttCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATc                                                                                    <  1:310820/251‑1 (MQ=255)                                                                                                                                                          taaAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCgg                                                                          <  1:195466/251‑1 (MQ=255)                                                                                                                                                          taaAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCgg                                                                          <  1:540235/251‑1 (MQ=255)                                                                                                                                                               gCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGtttt                                                                                                                                                                              <  1:534342/146‑1 (MQ=255)                                                                                                                                                               gCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGaaa                                                                     >  1:611529/1‑251 (MQ=255)                                                                                                                                                                          gTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGAc                                                                                                                                         <  1:632623/172‑1 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCg                                                                                                          >  1:165220/1‑184 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAAtt                                                    <  1:198842/238‑1 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATgag                                            <  1:76239/246‑1 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATg                                        >  1:676291/1‑250 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATg                                        >  1:463312/1‑250 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATg                                        >  1:320619/1‑250 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATg                                        >  1:400765/1‑250 (MQ=255)                                                                                                                                                                                             gCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGCTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATg                                        >  1:118409/1‑250 (MQ=255)                                                                                                                                                                                               gTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCAc                                                                                  >  1:37146/1‑206 (MQ=255)                                                                                                                                                                                               gTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATc                                                                            >  1:372506/1‑212 (MQ=255)                                                                                                                                                                                                tAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGAc                                                                                                                                         <  1:383849/150‑1 (MQ=255)                                                                                                                                                                                                tAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAAtt                                                    >  1:535735/1‑235 (MQ=255)                                                                                                                                                                                                  atatGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTc                                                                                                                                                   >  1:159131/1‑138 (MQ=255)                                                                                                                                                                                                  atatGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGAc                                                                                                                      <  1:732592/167‑1 (MQ=255)                                                                                                                                                                                                  atatGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGaa                                          >  1:535199/1‑243 (MQ=255)                                                                                                                                                                                                  atatGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAg                                  >  1:579857/1‑251 (MQ=255)                                                                                                                                                                                                  atatGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAg                                  >  1:879538/1‑251 (MQ=255)                                                                                                                                                                                                  atatGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAAATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTg                                                   >  1:429930/1‑234 (MQ=255)                                                                                                                                                                                                     tGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGAcc                               <  1:772565/251‑1 (MQ=255)                                                                                                                                                                                                            gttgtAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGaa                                          >  1:598762/1‑233 (MQ=255)                                                                                                                                                                                                            gttgtAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTc                         >  1:49954/1‑250 (MQ=255)                                                                                                                                                                                                              tgtAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCAc                      <  1:604428/251‑1 (MQ=255)                                                                                                                                                                                                               gtAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATc                                                                                    <  1:304811/188‑1 (MQ=255)                                                                                                                                                                                                                tAAATAAGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCACg                     <  1:696857/250‑1 (MQ=255)                                                                                                                                                                                                                     aaGCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCACGGTTAac               <  1:862518/251‑1 (MQ=255)                                                                                                                                                                                                                       gCATTGGCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAAc                                                                                                                                                               >  1:759798/1‑105 (MQ=255)                                                                                                                                                                                                                            ggCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCgg                                                                          <  1:430831/185‑1 (MQ=255)                                                                                                                                                                                                                            ggCTTGGGAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTc                         <  1:681664/234‑1 (MQ=255)                                                                                                                                                                                                                                  ggAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCACGGTTAACACCGAATTCAGCa  >  1:322556/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggAGAAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCACGGTTAACACCGAATTCAGCa  >  1:543661/1‑251 (MQ=255)                                                                                                                                                                                                                                     gaAATGACGGCAACCGAAAAAACCGCGATGGGCGGACAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGAc                                <  1:29410/218‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 ATCCGCCGGTGTCAGGCGGCGGCAAATGTCGATTAATTGTTGGCTTTGCGCCAAAAAGTGCGGTTGAAATTTCGGGAGTTTTTCCGGCACTGCGGACTGAAAATCTAAGGTTTTTGCCGGAGAAATAATCGCGAGCATAAAAATTCCTATTAATAAAAGCTAAATTGAAGTTAATTGCCAGTTAATCGGCGTAATATGGTGTTGTTGTAAATAAGCATTGGCTTGGGAGAAATGATGGCAACCGAAAAAACCGCGATGGGCGGATAACGGCGAAGGATGCACGGATGTCAGCACGCAATGTTTTTGTCTGTCAATAAACCGCCCTTTTTTCTGCGCCGGACTGCCCCACAATAAAAAGACCAGATTTTCCCGTTGTGAATTCAAGGTTTCAATCACTTTATCGGTGAAAATTTCCCAGCCCCAATTGGCATGAGAATGCGCCAGACCTTGTTCCACGGTTAACACCGAATTCAGCA  >  1/2098694‑2099169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑