BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,069,301	G→C	intergenic (‑13418/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,069,301	0	G	C	98.4%	159.1 / ‑5.7	62	intergenic (‑13418/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (15/45); minor base T (1/0); total (17/45)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TTGGCAAAAATTCTTTTTCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACGTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACGGCTTGCGTGAGCG  >  1/2069084‑2069538

ttGGCAAAAATTCTTTTTCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAGCCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGtt                                                                                                                                                                                                              <  1:385274/251‑1 (MQ=255)

          ttCTTTTTCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGGTTAGGATTATCCCGTGG‑TTTAACTTGG‑GGGTA‑TTTCCAttttttttggtggctgtctcctttata                                                                                                                                                                                                      >  1:477142/1‑226 (MQ=255)

              ttttCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGAt                                                                                                                                                                                                <  1:151212/251‑1 (MQ=255)

               tttCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATg                                                                                                                                                                                               <  1:619457/251‑1 (MQ=255)

               tttCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATg                                                                                                                                                                                               <  1:616308/251‑1 (MQ=255)

                      gCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑ggg                                                                                                                                                                                                                      <  1:44018/221‑1 (MQ=255)

                         ttGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCaaaa                                                                                                                                                                                      <  1:348694/250‑1 (MQ=255)

                           ggCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTTGCATTATCCCCT‑G‑GTTTACCTGGCGGATATTTGCC‑TTTGTGT‑GGGGGGCGGGTTGATTGCCTTTGGTGCGGTCaaaaaag                                                                                                                                                                                   >  1:242470/1‑249 (MQ=255)

                                gctgttattctccgctatcggTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGGTTGGGATTTTCCCCC‑G‑TTTAACCTGGCGGGGA‑TTTCCATTTTTGG‑GGGTGGCCGTTTGTTTGTCTTGGGTGGGTTCATAAAAGGccaa                                                                                                                                                                              >  1:100021/20‑248 (MQ=255)

                                        tCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGGTTGGCATTTTTCCCT‑G‑TTTTACCTTGCGGGTA‑TTTCCCTTTTTGTGGGGGGCCGGTTTGTTTCCTTTGGTGGGGTTC‑AAAACGGCCATAAAAAGGa                                                                                                                                                                      >  1:861696/1‑251 (MQ=255)

                                              ttGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTaa                                                                                                                                                                <  1:810060/250‑1 (MQ=255)

                                                 ttGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAaata                                                                                                                                                             <  1:50596/251‑1 (MQ=255)

                                                                   aaaaaTCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGcc                                                                                                                                           <  1:618634/251‑1 (MQ=255)

                                                                               gAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTTGCATTTTTCCGT‑G‑GTTTACCTGGCGGGTA‑TTGCCATTTGTGT‑GGGGGGCGGTTTTATTGTCTTTGGTGCGGTCCAAAAAGGCAATAAACAAGGATGTTAATAATGAAAAATATTTTTGcctgtcttttttta                                                                                                                               >  1:741601/1‑239 (MQ=255)

                                                                                  cAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTc                                                                                                                            <  1:639437/251‑1 (MQ=255)

                                                                                                  ccgccTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGAt                                                                                                             <  1:825750/250‑1 (MQ=255)

                                                                                                  ccgccTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGAt                                                                                                             <  1:154716/250‑1 (MQ=255)

                                                                                                         cGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGAt                                                                                                                                                                                                <  1:150511/160‑1 (MQ=255)

                                                                                                               gATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGaa                                                                                                                                                                     <  1:342340/181‑1 (MQ=255)

                                                                                                                        cggcgCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAAc                                                                                       <  1:770281/250‑1 (MQ=255)

                                                                                                                        cggcgCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAAc                                                                                       <  1:535753/250‑1 (MQ=255)

                                                                                                                        cggcgCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAAc                                                                                       <  1:72521/250‑1 (MQ=255)

                                                                                                                        cggcgCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAAc                                                                                       <  1:436471/250‑1 (MQ=255)

                                                                                                                        cggcgCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAAc                                                                                       <  1:414298/250‑1 (MQ=255)

                                                                                                                            gcTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGc                                                                                                        <  1:849733/229‑1 (MQ=255)

                                                                                                                                         cggcggATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCAc                                                                      <  1:857734/250‑1 (MQ=255)

                                                                                                                                                 cGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTTACCTGGCGGGTA‑TTGCCATTTTTGT‑GGGGGGCGGGTTTATTGCCTTTGGTGCGTTCAAAAAAGGCAATAAAAAGGAATGTaa                                                                                                                                                                >  1:667062/1‑152 (MQ=255)

                                                                                                                                                 cGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGAt                                                                                                             <  1:112587/203‑1 (MQ=255)

                                                                                                                                                 cGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTgg                                                                                    <  1:260058/228‑1 (MQ=255)

                                                                                                                                                  gTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGc                                                                                                 <  1:693364/214‑1 (MQ=255)

                                                                                                                                                      gTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGGATTATCCCGG‑G‑TTTAAACCGGCGGGTA‑TTGGCCTTTGTGG‑GGGGGCCGGTTTGGTTGCCTTGGATGCGGTCAAAAAAGGCAATAAAAAGGAATGGAAATAATGAAAAATATTTTAACCAACCCGCGCGGGGTCTTTGCCGGCGGGGGTCGTGCTATCAGCATCGTAGAAATGGCGGTGGGAATTCACGGTGCGCCCCtttt                                                        >  1:191372/1‑250 (MQ=255)

                                                                                                                                                      gTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCCTTATTCCCT‑GTTTTAAC‑TTGTGGGTA‑TTGCCATTTGTGT‑GGGTGGCGGTTTGTTTTCCTTTGGTGCGTTTTAAAACGGCAATAAAAAGGAATTTAAATAATGAAAAATATTTTAGCCCACCCCCGCGGGGTCTTTGCCGGCGTGGATTGTGCTATCCGCATCGTAGAAATGGCGCTGGGAATTTACGGTggggccctttt                                                        >  1:509353/1‑241 (MQ=255)

                                                                                                                                                      gTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGaa                                                                                                                                                                     <  1:618656/142‑1 (MQ=255)

                                                                                                                                                      gTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGaa                                                                                                                                                                     <  1:356550/142‑1 (MQ=255)

                                                                                                                                                      gTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCCT‑G‑TTTTACCTGGCGGGTA‑TTTCCCTTTTTGT‑GGGTGGCGGGTTTATTGCCTTGGGTGCGTTTAAAAACGGCAATAAAAAGGAATGTAAATAATGAAAAATATTTTATCCAACCCGCGCGGGGTCTGTGCCGGCGGGGATCGTGCTATCAGCCTCGTAGAAATGGCGGTGGGAATTCACGGGGCGCCCAtttt                                                        >  1:461264/1‑250 (MQ=255)

                                                                                                                                                       tATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTa                                                        <  1:390859/250‑1 (MQ=255)

                                                                                                                                                       tATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTa                                                        <  1:202427/250‑1 (MQ=255)

                                                                                                                                                        aTCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTAtg                                                      <  1:167232/251‑1 (MQ=255)

                                                                                                                                                         tCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTAtgt                                                     <  1:728413/251‑1 (MQ=255)

                                                                                                                                                              gCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCgg                                                                                                                    <  1:571888/183‑1 (MQ=255)

                                                                                                                                                              gCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCa                                                            <  1:510656/239‑1 (MQ=255)

                                                                                                                                                                      gtggATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGGGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAgt                                         <  1:359591/250‑1 (MQ=255)

                                                                                                                                                                        ggATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGc                                                   <  1:504915/238‑1 (MQ=255)

                                                                                                                                                                          aTTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAgtggtg                                     <  1:154284/250‑1 (MQ=255)

                                                                                                                                                                               ttAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAAc                               <  1:418163/251‑1 (MQ=255)

                                                                                                                                                                                         aTTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGTGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTGTGTGCGGCATGAAGTGGTGCATAACCGTTTCgtgg                     <  1:423688/251‑1 (MQ=255)

                                                                                                                                                                                                ggATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAAc                                                                                                                                                                                    >  1:864979/1‑85 (MQ=255)

                                                                                                                                                                                                ggATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATATTGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGc                                    <  1:128733/229‑1 (MQ=255)

                                                                                                                                                                                                ggATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCg                             >  1:40003/1‑236 (MQ=255)

                                                                                                                                                                                                ggATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCgtgg                     <  1:249621/244‑1 (MQ=255)

                                                                                                                                                                                                 gATTGGCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACgg              <  1:563113/250‑1 (MQ=255)

                                                                                                                                                                                                     ggCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTCTGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACGGCTTg          >  1:145050/1‑250 (MQ=255)

                                                                                                                                                                                                      gCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTaaa                                                                                                                                                               >  1:271853/1‑100 (MQ=255)

                                                                                                                                                                                                      gCATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCg                                                                                  <  1:619561/177‑1 (MQ=255)

                                                                                                                                                                                                       cATTATCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACGGCTTGCg        <  1:358032/250‑1 (MQ=255)

                                                                                                                                                                                                          gaTCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAg                                          >  1:751628/2‑213 (MQ=255)

                                                                                                                                                                                                           aTCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGc                                                   <  1:111965/203‑1 (MQ=255)

                                                                                                                                                                                                           aTCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGGGCATAACCGTTTCGTGGTGAACGGCTTGCGTGAg    >  1:310391/1‑250 (MQ=255)

                                                                                                                                                                                                            tCCCGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACGGCTTGCGTGAgcg  <  1:668943/251‑1 (MQ=255)

                                                                                                                                                                                                             cccGT‑G‑TTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACGGCTTGCGTGAgcg  >  1:542494/1‑250 (MQ=255)

                                                                                                                                                                                                                gt‑g‑tTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCAc                                                                      >  1:895379/1‑179 (MQ=255)

                                                                                                                                                                                                                   g‑tTTAACCTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAAc                <  1:380074/231‑1 (MQ=255)

TTGGCAAAAATTCTTTTTCCTTGCGTTGGCAGTGGGGATTTCCGCCATGTTGGTTTATTTTTTAATGAAAAATCGCCATGAACAAAAACTGCTGAATGCCGCCTACGCTTTGATTATCGGCGGCGCTTTGGGCAATGCGGCGGATCGTCTGTATCACGGCTATGTGGTGGATTTTTTAGATTTCTATTGGCGGGATTGGCATTATCCCGT‑G‑TTTAACGTGGCGGATA‑TTGCCATTTGTGT‑GGGTGCCGGTTTGATTGCCTTGGATGCGTTCAAAAACGGCAATAAACAGGAATGTAAATAATGAAAATTATTTTAGCCAACCCGCGCGGGTTCTGTGCCGGCGTGGATCGTGCTATCAGCATCGTAGAACTGGCGTTGGAAATTCACGGTGCGCCCATTTATGTGCGGCATGAAGTGGTGCATAACCGTTTCGTGGTGAACGGCTTGCGTGAGCG  >  1/2069084‑2069538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`