Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,062,615	T→C	intergenic (‑6732/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,062,615	0	T	C	100.0%	183.9 / NA	64	intergenic (‑6732/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/37);  total (27/37)

TCGGAAATAAACACAATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGTATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCGGTGAGACACAACCGAAAGAAAGCATCGTTATAT  >  1/2062386‑2062859                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tCGGAAATAAACACAATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGt                                                                                                                                                                                                                                 <  1:701794/251‑1 (MQ=255)     aaaTAAACACAATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGc                                                                                                                                                                                                                             <  1:404994/251‑1 (MQ=255)      aaTAAACACAATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGca                                                                                                                                                                                                                            <  1:634121/251‑1 (MQ=255)         aaaCACAATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGcatca                                                                                                                                                                                                                         >  1:268993/1‑251 (MQ=255)              caATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATagag                                                                                                                                                                                                                    >  1:591395/1‑251 (MQ=255)               aaTTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGc                                                                                                                                                                                                                   <  1:573963/251‑1 (MQ=255)               aaTTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGc                                                                                                                                                                                                                   <  1:241987/251‑1 (MQ=255)               aaTTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTCCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGc                                                                                                                                                                                                                   <  1:4306/251‑1 (MQ=255)                      aaaaaCAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCt                                                                                                                                                                                                            <  1:255346/251‑1 (MQ=255)                         aaCAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATag                                                                                                                                                                                                                      >  1:290906/1‑238 (MQ=255)                                aaCCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTgtgt                                                                                                                                                                                                  <  1:521709/251‑1 (MQ=255)                                     cTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGAc                                                                                                                                                                                              <  1:266132/250‑1 (MQ=255)                                      tCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACgg                                                                                                                                                                                            <  1:167324/251‑1 (MQ=255)                                         aTTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGca                                                                                                                                                                                                                            >  1:201938/1‑216 (MQ=255)                                         aTTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATagag                                                                                                                                                                                                                    <  1:484711/224‑1 (MQ=255)                                                ggTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTa                                                                                                                                                                                                                                >  1:171474/1‑205 (MQ=255)                                                ggTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGcatcat                                                                                                                                                                                                                        >  1:634701/1‑213 (MQ=255)                                                       ccTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAAc                                                                                                                                                                                                                                                <  1:361395/182‑1 (MQ=255)                                                                    gCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGca                                                                                                                                                                                                                            <  1:11494/189‑1 (MQ=255)                                                                              tGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGa                                                                                                                                                    >  1:158478/1‑251 (MQ=255)                                                                                     tttttGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGc                                                                                                                                                                                                                             <  1:713438/171‑1 (MQ=255)                                                                                      ttttGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGcc                                                                                                                                            <  1:821796/251‑1 (MQ=255)                                                                                          gATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGc                                                                                                                                                                                                             >  1:523060/1‑182 (MQ=255)                                                                                                    gCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGc                                                                                                                                                                                                                   >  1:773267/1‑166 (MQ=255)                                                                                                     cTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTc                                                                                                                                <  1:513631/248‑1 (MQ=255)                                                                                                       aaTTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACt                                                                                                                                                                                                                                               >  1:786298/1‑135 (MQ=255)                                                                                                       aaTTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTaaa                                                                                                                           >  1:544404/1‑251 (MQ=255)                                                                                                         tttAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTaaaaa                                                                                                                         >  1:835609/1‑251 (MQ=255)                                                                                                             gATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGc                                                                                                                                                                                                                   >  1:525891/1‑157 (MQ=255)                                                                                                                     gTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTgtgt                                                                                                                                                                                                  <  1:70855/166‑1 (MQ=255)                                                                                                                          ctgctTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAg                                                                                                        <  1:384862/251‑1 (MQ=255)                                                                                                                          ctgctTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAg                                                                                                        <  1:411023/251‑1 (MQ=255)                                                                                                                            gctTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTg                                                                                                      >  1:731387/1‑251 (MQ=255)                                                                                                                                   tCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAAt                                                                                               <  1:96673/251‑1 (MQ=255)                                                                                                                                      cgTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAgg                                                                                            <  1:547023/251‑1 (MQ=255)                                                                                                                                          gATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTg                                                                                                                                                                                                                                         <  1:772435/106‑1 (MQ=255)                                                                                                                                          gATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGatat                                                                                                                                                 <  1:674603/194‑1 (MQ=255)                                                                                                                                          gATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCg                                                                                        >  1:422724/1‑251 (MQ=255)                                                                                                                                          gATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCg                                                                                        >  1:271321/1‑251 (MQ=255)                                                                                                                                             tAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTaa                                                                                     <  1:226248/251‑1 (MQ=255)                                                                                                                                              aaaCCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTaaa                                                                                    <  1:888068/251‑1 (MQ=255)                                                                                                                                               aaCCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAat                                                                                   <  1:118714/251‑1 (MQ=255)                                                                                                                                               aaCCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAat                                                                                   <  1:532348/251‑1 (MQ=255)                                                                                                                                                 ccTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAg                                                                                                        >  1:230144/1‑228 (MQ=255)                                                                                                                                                 ccTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAatat                                                                                 >  1:611417/1‑251 (MQ=255)                                                                                                                                                 ccTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAatat                                                                                 >  1:384737/1‑251 (MQ=255)                                                                                                                                                    ttAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCGAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGc                                                                               >  1:598142/1‑250 (MQ=255)                                                                                                                                                       gATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGt                                                                                                                                       >  1:464769/1‑191 (MQ=255)                                                                                                                                                                cTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATAc                                                                  <  1:380094/251‑1 (MQ=255)                                                                                                                                                                cTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATAc                                                                  <  1:800690/251‑1 (MQ=255)                                                                                                                                                                  gTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAgg                                                                                            <  1:674622/223‑1 (MQ=255)                                                                                                                                                                  gTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACtt                                                                >  1:889124/1‑251 (MQ=255)                                                                                                                                                                       caACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTc                                                           <  1:331190/251‑1 (MQ=255)                                                                                                                                                                         aCTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGc                                                                                                                                                                                                             <  1:240732/103‑1 (MQ=255)                                                                                                                                                                                          gAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTc                                                                                                                                      <  1:127675/157‑1 (MQ=255)                                                                                                                                                                                                ggCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAAc                                                                                                                                  >  1:228700/1‑155 (MQ=255)                                                                                                                                                                                                ggCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACtt                                                                >  1:458512/1‑221 (MQ=255)                                                                                                                                                                                                ggCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCgg                                  >  1:393543/1‑251 (MQ=255)                                                                                                                                                                                                 gCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCGGt                                 >  1:386436/1‑251 (MQ=255)                                                                                                                                                                                                     cagcagCTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCGGTgaga                             >  1:594677/1‑251 (MQ=255)                                                                                                                                                                                                        cagcTGCTTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCGGTGAGac                            <  1:647015/249‑1 (MQ=255)                                                                                                                                                                                                          gctgctTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTaaaa                                                                                                                          <  1:453472/153‑1 (MQ=255)                                                                                                                                                                                                           ctgctTTCTCGCCTGCGGCAACTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATAc                                                                  <  1:354895/208‑1 (MQ=255)                                                                                                                                                                                                                                cTTGTGCATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCGGTGAGACACAACCGAAAGAAAGCATCGTTatat  <  1:659687/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TCGGAAATAAACACAATTGATAAAAACAAAAAACCGCTCAATTGAGCGGTTTCTCCTTATTTGCTAAGCAAAAATTATGCTAATTTTTTGATTTGTGCAGCTAATTTAGATTTGTGGTTGGCTGCTTTGTTCGCGTGGATTAAACCTTTAGATGCCATTCTGTCCACAACTTTTTGCATTTCAACGAATGCGGCTTCAGCAGCTGCTTTCTCGCCTGCGGCAACTTGTGTATAAACTTTTTTGATGTAAGTACGCATCATAGAGCGTTGGCTTGCGTTGTGTTGACGGCGTTTTTCAGATTGAACCGCACGTTTTTTTGCTGACTTGATATTAGCCAAGGTCAAACTCCTAAAAATTTCTGTTAGTTAATAGTGACAAAATAGGGGCGTAAATATGCCGATTTCTTATACTTTTGTCAACGGGAATTTTGTGTTGATTTCGGTGAGACACAACCGAAAGAAAGCATCGTTATAT  >  1/2062386‑2062859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑