Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 2,030,104 T→G intergenic (‑37396/+10573) unknown ← / ← unknown tRNA‑Lys‑TTT/tRNA‑Ser‑TGA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*12,030,1040TG100.0% 83.0 / NA 30intergenic (‑37396/+10573)unknown/unknowntRNA‑Lys‑TTT/tRNA‑Ser‑TGA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/9);  total (21/9)

GATTGCTATAACATAGCGCCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTTCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATCCCAGAAGCAGAAGTCGCTTATCAAT  >  1/2029867‑2030349
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
gATTGCTATAACATAGCGCCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCAttt                                                                                                                                                                                                                                          >  1:106488/1‑251 (MQ=255)
    gCTATAACATAGCGCCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCAttttt                                                                                                                                                                                                                                        >  1:70549/1‑249 (MQ=255)
    gCTATAACATAGCGCCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCAttttt                                                                                                                                                                                                                                        >  1:340151/1‑249 (MQ=255)
     cTATAACATAGCGCCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCAttttt                                                                                                                                                                                                                                        >  1:47836/1‑248 (MQ=255)
                cgcCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACATATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGa                                                                                                                                                                                                                           <  1:451548/250‑1 (MQ=255)
                       tattGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCAt                                                                                                                                                                                                                   >  1:71375/1‑251 (MQ=255)
                                                                    ttACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCa                                                                                                                                                                                              <  1:36187/227‑1 (MQ=255)
                                                                           aaaGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCAtt                                                                                                                                                               >  1:155464/1‑251 (MQ=255)
                                                                                                  ttACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGt                                                                                                                                        >  1:761703/1‑251 (MQ=255)
                                                                                                       caGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGAt                                                                                                                                                                                                                                               <  1:285964/143‑1 (MQ=255)
                                                                                                          gAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATgc                                                                                                                                  <  1:199767/249‑1 (MQ=255)
                                                                                                                     ttATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGc                                                                                                                                             >  1:99316/1‑227 (MQ=255)
                                                                                                                                     tttttAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACt                                                                                                                                                                            >  1:527666/1‑180 (MQ=255)
                                                                                                                                               aaaCATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAAcaca                                                                                           >  1:883859/1‑251 (MQ=255)
                                                                                                                                                 aCATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCt                                                                                                                                                >  1:223930/1‑196 (MQ=255)
                                                                                                                                                        ttttCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGt                                                                                  <  1:764349/251‑1 (MQ=255)
                                                                                                                                                              ccTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATcaa                                                                            >  1:183143/1‑251 (MQ=255)
                                                                                                                                                                             tttttATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAatat                                                                                     <  1:285668/227‑1 (MQ=255)
                                                                                                                                                                                ttATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAAcc                                                                                                                                                                  <  1:742647/147‑1 (MQ=255)
                                                                                                                                                                                        gagTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTg                                                  >  1:15242/1‑251 (MQ=255)
                                                                                                                                                                                          gTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGc                                                              >  1:604314/1‑237 (MQ=255)
                                                                                                                                                                                             aataaatCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCg                                             >  1:533354/1‑251 (MQ=255)
                                                                                                                                                                                                      ttCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAat                                                                                       >  1:873607/1‑200 (MQ=255)
                                                                                                                                                                                                                 gAATTGATCTTTACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATcc                          >  1:185389/1‑250 (MQ=255)
                                                                                                                                                                                                                           ttACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATCCcagaagcagaa               >  1:148448/1‑251 (MQ=255)
                                                                                                                                                                                                                           ttACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATCCcagaagcagaa               >  1:218730/1‑251 (MQ=255)
                                                                                                                                                                                                                            tACAAAATAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATCCcagaagcagaag              <  1:773813/251‑1 (MQ=255)
                                                                                                                                                                                                                                aaaTAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTg                                                                                                                                                              >  1:367133/1‑103 (MQ=255)
                                                                                                                                                                                                                                aaaTAACTATTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACggg                              <  1:554249/231‑1 (MQ=255)
                                                                                                                                                                                                                                        aTTTTGCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATCCCAGAAGCAGAAGTCGCTTATCAat  >  1:124583/1‑251 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
GATTGCTATAACATAGCGCCGATTATTGGTAAAACGGGTTACATGATGAAATACGTAATTTTCTTGCATTACTGGAAAGCCAAGGGAGCTCAAACACATTACACAGGAAATCGGTCCTTATTTAGAGGCGAAATTTTTAAATAAAACATAATTTTTCGCCTTTATTTTTTCCTTTTTTATAGAAGAGTAAATAAATCGTTCTTTTTTGTGAATTGATCTTTACAAAATAACTATTTTTCGATAGATCATTTTTCTGACTCTTACGATAATTCATTGCTCATAACAAGGAGATTCAAATGCAACAAAAATTACTTGTCTCAACCATTGCGCTTGCCTTATCTTGCAGCGTTTATGCCGCCAAAGTACCTGAAGGAACACAGCTCGCAGAAACACAGAATATCGTGATCAACAACGGTTCCGAGCCACAAAGTTTTGACCCGCACAAAACCGACGGGATCCCAGAAGCAGAAGTCGCTTATCAAT  >  1/2029867‑2030349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑