Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,028,855	A→G	intergenic (‑36147/+11822)	unknown ← / ← unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,028,855	0	A	G	100.0%	192.9 / NA	67	intergenic (‑36147/+11822)	unknown/unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/38);  total (29/38)

TCGCCGATTATCCGTTTACCACCTTGGTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCAGAATTGTTCCAATACAGCGACAGCGAGTCCTTGGCGGAAAAACCGCGCTGGCTGGTGTTCAACAAAATCGATACGCTCAGTGATGAAGAAGCCCATGCGCGAGCGAAAGAGATCACCGAACGTCTGGGCCGGGAGGAAGGTTATTATTTAATTTCCGCCGCCACCGGTAAAAACGCCCCGCAACTGTGCCGCGATATTATGGACTTCCTGGAAGCCCACCCGC  >  1/2028620‑2029078                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                tCGCCGATTATCCGTTTACCACCTTGGTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATac                                                                                                                                                                                                                  >  1:364565‑M2/1‑249 (MQ=255) tCGCCGATTATCCGTTTACCACCTTGGTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATac                                                                                                                                                                                                                  >  1:473217‑M2/1‑249 (MQ=255)          aTCCGTTTACCACCTTGGTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTAGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtc                                                                                                                                                                                                          <  1:432900‑M2/250‑11 (MQ=255)               tttACCACCTTGGTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggc                                                                                                                                                                                                    >  1:678171‑M2/1‑235 (MQ=255)                          ggTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccg                                                                                                                                                                                          <  1:643003‑M2/250‑27 (MQ=255)                           gTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcg                                                                                                                                                                                        <  1:877077‑M2/251‑29 (MQ=255)                           gTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATac                                                                                                                                                                                                                  <  1:42952‑M2/225‑3 (MQ=255)                             aCCAAGTTTAGGCGTGGTGAAAGTAGAAGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcg                                                                                                                                                                                        <  1:111792‑M2/249‑29 (MQ=255)                                 aGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGCGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctgg                                                                                                                                                                                    <  1:285764‑M2/249‑33 (MQ=255)                                  gTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctgg                                                                                                                                                                                    <  1:527201‑M2/248‑33 (MQ=255)                                   tttAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggct                                                                                                                                                                                  >  1:509153‑M2/1‑215 (MQ=255)                                   tttAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggct                                                                                                                                                                                  >  1:91569‑M2/1‑215 (MQ=255)                                           tggtgAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATac                                                                                                                                                                                                                  <  1:349862‑M2/209‑3 (MQ=255)                                            ggtgAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgt                                                                                                                                                                             >  1:336240‑M2/1‑206 (MQ=255)                                                aaaGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATTGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAAAAATTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATAAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcaagtccttggcggaaaaaccgcgctggctggtgttcaacaaaat                                                                                                                                                                   >  1:712235‑M2/1‑202 (MQ=255)                                                aaaGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaat                                                                                                                                                                   >  1:201247‑M2/1‑202 (MQ=255)                                                 aaGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATac                                                                                                                                                                                                                  <  1:865511‑M2/203‑3 (MQ=255)                                                        tGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgc                                                                                                                                                           >  1:291550‑M2/1‑194 (MQ=255)                                                               cGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtga                                                                                                                                                    >  1:413279‑M2/1‑187 (MQ=255)                                                               cGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtga                                                                                                                                                    >  1:830120‑M2/1‑187 (MQ=255)                                                                 cAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatg                                                                                                                                                  <  1:134771‑M2/251‑67 (MQ=255)                                                                   gTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaa                                                                                                                                                                      <  1:602564‑M2/229‑47 (MQ=255)                                                                    ttttGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaag                                                                                                                                               >  1:847607‑M2/1‑182 (MQ=255)                                                                    ttttGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatga                                                                                                                                                 >  1:661147‑M2/1‑182 (MQ=255)                                                                            tCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtg                                                                                                                                                                              <  1:321178‑M2/212‑39 (MQ=255)                                                                             cGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatg                                                                                                                                      >  1:386385‑M2/1‑173 (MQ=255)                                                                                     ttCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcg                                                                                                                              <  1:554037‑M2/251‑87 (MQ=255)                                                                                     ttCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcg                                                                                                                              <  1:773411‑M2/251‑87 (MQ=255)                                                                                            cTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaag                                                                                                                                               >  1:801651‑M2/1‑158 (MQ=255)                                                                                               aaTTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatca                                                                                                                    <  1:321556‑M2/251‑97 (MQ=255)                                                                                               aaTTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaag                                                                                                                                               <  1:890261‑M2/224‑70 (MQ=255)                                                                                                             cGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggc                                                                                                      >  1:765280‑M2/1‑141 (MQ=255)                                                                                                              ggAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccga                                                                                                                <  1:813779‑M2/240‑101 (MQ=255)                                                                                                               gAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccg                                                                                                    <  1:544289‑M2/251‑113 (MQ=255)                                                                                                               gAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTAACGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccg                                                                                                    <  1:409037‑M2/251‑113 (MQ=255)                                                                                                                   gAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggag                                                                                                <  1:795238‑M2/251‑117 (MQ=255)                                                                                                                        ggCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtga                                                                                                                                                    <  1:558328‑M2/194‑65 (MQ=255)                                                                                                                                tATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattat                                                                                    >  1:572816‑M2/1‑122 (MQ=255)                                                                                                                                tATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacaccgattccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattat                                                                                    >  1:611582‑M2/1‑122 (MQ=255)                                                                                                                                         ccTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatc                                                                                                                     <  1:298575‑M2/208‑96 (MQ=255)                                                                                                                                                cacTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaac                                                                                                              <  1:554295‑M2/208‑103 (MQ=255)                                                                                                                                                  cTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgata                                                                                                                                                              <  1:295228‑M2/158‑55 (MQ=255)                                                                                                                                                                  gtgtTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgca                                                 <  1:667067‑M2/251‑164 (MQ=255)                                                                                                                                                                     ttGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaa                                                          >  1:269327‑M2/1‑85 (MQ=255)                                                                                                                                                                      tGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactg                                             >  1:100327‑M2/1‑84 (MQ=255)                                                                                                                                                                       gATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaa                                                <  1:520852‑M2/247‑165 (MQ=255)                                                                                                                                                                         ttCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtg                                           <  1:535325‑M2/250‑170 (MQ=255)                                                                                                                                                                         ttCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtg                                           <  1:214399‑M2/250‑170 (MQ=255)                                                                                                                                                                                 gtggATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatat                                  <  1:207431‑M2/251‑179 (MQ=255)                                                                                                                                                                                 gtggATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgc                                          <  1:474514‑M2/243‑171 (MQ=255)                                                                                                                                                                                 gtggATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgcccagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatat                                  <  1:89276‑M2/251‑179 (MQ=255)                                                                                                                                                                                      tatCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatgg                             <  1:417614‑M2/251‑184 (MQ=255)                                                                                                                                                                                      tatCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatgg                             >  1:276682‑M2/1‑68 (MQ=255)                                                                                                                                                                                      tatCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatgg                             >  1:720892‑M2/1‑68 (MQ=255)                                                                                                                                                                                        tCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggac                           >  1:92549‑M2/1‑66 (MQ=255)                                                                                                                                                                                        tCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggac                           >  1:3875‑M2/1‑66 (MQ=255)                                                                                                                                                                                        tCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggac                           >  1:341828‑M2/1‑66 (MQ=255)                                                                                                                                                                                              cAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttc                        <  1:807703‑M2/248‑189 (MQ=255)                                                                                                                                                                                                aTTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttcctgga                   >  1:104175‑M2/1‑58 (MQ=255)                                                                                                                                                                                                  tgatgaTTCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttcctgga                   <  1:383971‑M2/249‑194 (MQ=255)                                                                                                                                                                                                        ttCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttcctggaagcccacc           >  1:179017‑M2/1‑50 (MQ=255)                                                                                                                                                                                                        ttCCAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttcctggaagcccac            <  1:708674‑M2/250‑201 (MQ=255)                                                                                                                                                                                                          ccAACCCGGCGGATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttcctggaagcccacccg         >  1:130593‑M2/1‑48 (MQ=255)                                                                                                                                                                                                                  gcggATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattat                                                                                    <  1:844117‑M2/168‑129 (MQ=255)                                                                                                                                                                                                                  gcggATAACGTGGCGATTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggcgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggttattatttaatttccgccgccaccggtaaaaacgccccgcaactgagccgcgatattatggacttcctggaagcccacccgcgcaaaa  <  1:78620‑M2/250‑211 (MQ=255)                                                                                                                                                                                                                                 aTTATCGAATCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaagaagcccatgcgcgagcgaaagagatcaccgaacgtctgggccgggaggaaggctattatttaatttccgccgccaccggtaaaaacgccccgcaactgtgccgcgatattatggacttcctgga                   <  1:491160‑M2/218‑194 (MQ=255)                                                                                                                                                                                                                                        aaTCGGAATTGTTCCAATacagcgagtccttggcggaaaaaccgcgctggctggtgttcaacaaaatcgatacgctcagtgatgaag                                                                                                                                               >  1:102461‑M2/1‑18 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                TCGCCGATTATCCGTTTACCACCTTGGTACCAAGTTTAGGCGTGGTGAAAGTAGATGACAATCGCAGTTTTGTGATCGCCGACATTCCTGGCTTAATTGAAGGCGCATCGGAAGGAGCAGGCTTGGGTATCCGCTTCCTGAAACACTTGGAACGTTGCCGCGTGTTGATTCATTTGGTGGATATCAACCCAATTGATGATTCCAACCCGGCGGATAACGTGGCGATTATCGAATCAGAATTGTTCCAATACAGCGACAGCGAGTCCTTGGCGGAAAAACCGCGCTGGCTGGTGTTCAACAAAATCGATACGCTCAGTGATGAAGAAGCCCATGCGCGAGCGAAAGAGATCACCGAACGTCTGGGCCGGGAGGAAGGTTATTATTTAATTTCCGCCGCCACCGGTAAAAACGCCCCGCAACTGTGCCGCGATATTATGGACTTCCTGGAAGCCCACCCGC  >  1/2028620‑2029078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑