Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,890,109	T→G	intergenic (+95182/‑44222)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,890,109	0	T	G	100.0%	219.6 / NA	77	intergenic (+95182/‑44222)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/38);  total (39/38)

CCCGATGCCACTTTTAAATAACACATCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTTAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAATATTCATAATGACC  >  1/1889862‑1890348                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                cccGATGCCACTTTTAAATAACACATCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAAc                                                                                                                                                                                                                                              <  1:186303/251‑1 (MQ=255)           cTTTTAAATAACACATCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATggg                                                                                                                                                                                                                                    >  1:15847/1‑251 (MQ=255)                      cacaTCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTaaa                                                                                                                                                                                                                           >  1:229086/1‑249 (MQ=255)                      cacaTCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACt                                                                                                                                                                                                                         <  1:267177/251‑1 (MQ=255)                        caTCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAgg                                                                                                                                                                                                                                         >  1:290673/1‑233 (MQ=255)                        caTCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTaaa                                                                                                                                                                                                                           >  1:116203/1‑247 (MQ=255)                        caTCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACtgt                                                                                                                                                                                                                       <  1:359917/251‑1 (MQ=255)                           cACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAAc                                                                                                                                                                                                                                              >  1:124887/1‑225 (MQ=255)                             ccGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGTTGGGCACATTAAACTGTTGttt                                                                                                                                                                                                                  >  1:826686/1‑251 (MQ=255)                             ccGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAAc                                                                                                                                                                                                                          >  1:851103/1‑243 (MQ=255)                             ccGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGttt                                                                                                                                                                                                                  >  1:281251/1‑251 (MQ=255)                               ggTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAAc                                                                                                                                                                                                                          >  1:882100/1‑241 (MQ=255)                               ggTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACt                                                                                                                                                                                                                         >  1:337668/1‑242 (MQ=255)                                            cGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGa                                                                                                                                                                                                                                        <  1:168178/214‑1 (MQ=255)                                            cGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAAc                                                                                                                                                                                                                          >  1:898306/1‑228 (MQ=255)                                              tttttGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCcgcg                                                                                                                                                                                                  >  1:126961/1‑250 (MQ=255)                                                      gTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGa                                                                                                                                                                                         >  1:65513/1‑251 (MQ=255)                                                        tCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATc                                                                                                                                                                                       <  1:13020/251‑1 (MQ=255)                                                         cGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATcc                                                                                                                                                                                      <  1:571231/251‑1 (MQ=255)                                                              aaaCCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACt                                                                                                                                                                                                                         <  1:858164/211‑1 (MQ=255)                                                                 ccGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATc                                                                                                                                                                                       >  1:520810/1‑242 (MQ=255)                                                                 ccGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGcc                                                                                                                                                                               >  1:392496/1‑250 (MQ=255)                                                                   gATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCAt                                                                                                                                                                            >  1:214314/1‑251 (MQ=255)                                                                        gCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCAtgagtg                                                                                                                                                                       >  1:642244/1‑251 (MQ=255)                                                                        gCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCAtgagtg                                                                                                                                                                       >  1:72438/1‑251 (MQ=255)                                                                             ggCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGAc                                                                                                                                                                                                               <  1:483513/206‑1 (MQ=255)                                                                               cGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCAtg                                                                                                                                                                           <  1:615196/240‑1 (MQ=255)                                                                                gtgtgtCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTACACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGATGTTTGACTTTTTCTGCCGCGCCCACATGATCCAACTGCCCATGAGTGAGTAAAAt                                                                                                                                                               >  1:377427/1‑251 (MQ=255)                                                                                gtgtgtCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACtg                                                                                                                                                                                                                        <  1:255952/194‑1 (MQ=255)                                                                                  gtgtCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCg                                                                                                                                                             >  1:26700/1‑251 (MQ=255)                                                                                  gtgtCCCGGTAAATGCAGAACCTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACt                                                                                                                                                                                                                         <  1:430516/191‑1 (MQ=255)                                                                                      cccGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGc                                                                                                                                                            >  1:579415/1‑248 (MQ=255)                                                                                         ggTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATg                                                                                                                                                                                 >  1:651266/1‑224 (MQ=255)                                                                                                      cTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAAt                                                                                                                                         >  1:539600/1‑251 (MQ=255)                                                                                                       ttCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAATAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAAtt                                                                                                                                        <  1:435855/251‑1 (MQ=255)                                                                                                              gTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAAGATGGGCACATTAAGCTGTTGTTTGTCTTTTTCCGCCGCGCCCACATGTTCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCaa                                                                                                                                  <  1:729786/250‑1 (MQ=255)                                                                                                                aaaaTCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTc                                                                                                                               <  1:559652/251‑1 (MQ=255)                                                                                                                   aTCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGtt                                                                                                                            >  1:471674/1‑251 (MQ=255)                                                                                                                           tAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAAt                                                                                                                    >  1:641420/1‑251 (MQ=255)                                                                                                                            aGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACt                                                                                                                                                                                                                         >  1:515081/1‑149 (MQ=255)                                                                                                                            aGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATcc                                                                                                                                                                                      >  1:196042/1‑184 (MQ=255)                                                                                                                            aGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAAtt                                                                                                                   >  1:25317/1‑251 (MQ=255)                                                                                                                              ttAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAAttt                                                                                                                  <  1:663694/250‑1 (MQ=255)                                                                                                                                 aaaTTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATcc                                                                                                              <  1:682013/251‑1 (MQ=255)                                                                                                                                  aaTTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGc                                                                                                                                                                                                                                   >  1:274528/1‑133 (MQ=255)                                                                                                                                   aTTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGc                                                                                                            <  1:558429/251‑1 (MQ=255)                                                                                                                                           ttcttTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTcgccg                                                                                                     >  1:211433/1‑250 (MQ=255)                                                                                                                                               ttATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAAttt                                                                                                                  <  1:174670/233‑1 (MQ=255)                                                                                                                                               ttATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGccgccg                                                                                                  <  1:815641/249‑1 (MQ=255)                                                                                                                                                    caaccaacGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCaa                                                                                           >  1:127882/1‑251 (MQ=255)                                                                                                                                                      accaacGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCaata                                                                                         >  1:162828/1‑251 (MQ=255)                                                                                                                                                       ccaacGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAAttc                                                                                                                                       >  1:283344/1‑204 (MQ=255)                                                                                                                                                       ccaacGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCaataa                                                                                        <  1:249601/251‑1 (MQ=255)                                                                                                                                                           cGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGc                                                                                    <  1:700177/251‑1 (MQ=255)                                                                                                                                                             aTCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCAtgag                                                                                                                                                                         <  1:8361/164‑1 (MQ=255)                                                                                                                                                                  gTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCAttt                                                                             >  1:312175/1‑251 (MQ=255)                                                                                                                                                                           cggcgACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTatc                                                                     <  1:238654/250‑1 (MQ=255)                                                                                                                                                                                aCTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAGGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCa                                                               >  1:441921/1‑251 (MQ=255)                                                                                                                                                                                    cAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAAtt                                                           <  1:898545/251‑1 (MQ=255)                                                                                                                                                                                    cAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAAtt                                                           <  1:275298/251‑1 (MQ=255)                                                                                                                                                                                    cAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAAtt                                                           <  1:411807/251‑1 (MQ=255)                                                                                                                                                                                    cAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGAACCAAGTGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAAtt                                                           <  1:174419/251‑1 (MQ=255)                                                                                                                                                                                        caacaTCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACg                                                       <  1:118445/251‑1 (MQ=255)                                                                                                                                                                                                   aTTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTttgt                                            <  1:823466/251‑1 (MQ=255)                                                                                                                                                                                                      tttCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGa                                         >  1:267528/1‑251 (MQ=255)                                                                                                                                                                                                                   cGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACg                             <  1:676421/250‑1 (MQ=255)                                                                                                                                                                                                                    ggCAAGCCCTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCaataa                                                                                        >  1:36022/1‑190 (MQ=255)                                                                                                                                                                                                                          cccTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATcc                                                                                                              <  1:570158/162‑1 (MQ=255)                                                                                                                                                                                                                          cccTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGaataa                      <  1:81965/250‑1 (MQ=255)                                                                                                                                                                                                                           ccTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATcc                                                                 >  1:189587/1‑206 (MQ=255)                                                                                                                                                                                                                           ccTTAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAAtt                    <  1:72638/251‑1 (MQ=255)                                                                                                                                                                                                                             ttAAACCAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTc                  <  1:374271/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAatat             >  1:640129/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccAATAATCATCTTTGGAATTTGAACCGAGGATGGGCACATGAAACTGTTGTTTGACTTTTTCCGCCGCGCCCGCATGATCCAAATACCCATGAGTGAGTAAAATCGCCTGTAGATTCAAGCCTAATTCTTCAATTCGTTTGAGTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAatat             <  1:577626/251‑1 (MQ=255)                                                                                                                                                                                                                                    aataatCATCTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAATATTc           <  1:691299/251‑1 (MQ=255)                                                                                                                                                                                                                                            tcTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAATATTCATAATGAc   <  1:858599/251‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTGGAATTTGAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAATATTCATAATGAcc  <  1:402421/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                CCCGATGCCACTTTTAAATAACACATCACCGGTAAACGCCACACGTTTTTGATGTTCGATAAAACCGATATGCCCCGGCGTGTGTCCCGGTAAATGCAGAACTTCAAAGGTAAAATCGCCGATAGTTAAAATTTCGCCTTCTTTATCCAACCAACGATCCGGTTCAAAGGCGGCGACTTCAAACAACATCCCGAATTTTTCCGATTGTTGCGGCAAGCCCTTAAACCAATAATCATCTTTGGAATTTTAACCGAGGATGGGCACATTAAACTGTTGTTTGACTTTTTCCGCCGCGCCCACATGATCCAAATGCCCATGAGTGAGTAAAATCGCCTGTAAATTCAAGCCTAATTCTTCAATTCGTTTGATTAATTTATCCGCTTCGCCGCCGGGATCAATAATTGCCGCATTTTTCTTATCATCCCAAATTAACGAACAATTTTGTTGAAATGCGGTGACGGGAATAATTTCAATATTCATAATGACC  >  1/1889862‑1890348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑