Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	375,593	C→T	intergenic (‑30670/+36283)	unknown ← / ← unknown	tRNA‑Ser‑GCT/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	375,593	0	C	T	100.0%	180.9 / NA	67	intergenic (‑30670/+36283)	unknown/unknown	tRNA‑Ser‑GCT/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/30);  total (37/30)

ATGGTAATCCCACGTTCTTGTTCCTGTTCCATCCAGTCCATGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATACGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATACCTAATG  >  1/375353‑375833                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 aTGGTAACCCCACGTTTTTGTTCCTGTTCCATCCAGTCCATGGTTGCGGCCCCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAAAACAAGATACGCTCAGTAGTAGTATTTTTCCCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGc                                                                                                                                                                                                                                         <  1:105141/250‑1 (MQ=255)                 tttGTTCCTGTTCCATCCAGTCCATGGTGGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAAAAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATTTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATg                                                                                                                                                                                                                        <  1:891388/250‑1 (MQ=255)                    tttcctgttccACCCAGTCCTGGGTTGCGGCACCATCGTGTACTGCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTAAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATgaga                                                                                                                                                                                                                     <  1:267607/250‑1 (MQ=255)                    gttcctgttccATCCAGTCCATGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATgaga                                                                                                                                                                                                                     >  1:420411/1‑251 (MQ=255)                     ttcctgttccATCCAGTCCATGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTCCCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATgaga                                                                                                                                                                                                                     <  1:422319/250‑1 (MQ=255)                              catccaGTCCATGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCaa                                                                                                                                                                                                           >  1:676799/1‑251 (MQ=255)                               atccaGTCCATGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCaaa                                                                                                                                                                                                          >  1:715313/1‑251 (MQ=255)                                        ttGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTATTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAAt                                                                                                                                                                                                 <  1:239339/250‑1 (MQ=255)                                           gTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATAc                                                                                                                                                                                               <  1:198479/250‑1 (MQ=255)                                           gTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATAc                                                                                                                                                                                               <  1:794928/250‑1 (MQ=255)                                                      aTCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATAtt                                                                                                                                                                                   >  1:892708/1‑251 (MQ=255)                                                      aTCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATAtt                                                                                                                                                                                   >  1:812886/1‑251 (MQ=255)                                                             aCTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCAc                                                                                                                                                                                                                                    >  1:108972/1‑195 (MQ=255)                                                                      tttttGTGACTTACGCCGGTAGAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCATTGATACCAATGTTGCGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGc                                                                                                                                                                   <  1:9445/250‑1 (MQ=255)                                                                      aTTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAg                                                                                                                                                                        >  1:603282/1‑246 (MQ=255)                                                                                   cacCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGc                                                                                                                                                                                                                                         >  1:238156/1‑168 (MQ=255)                                                                                     ccGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGc                                                                                                                                                    <  1:587483/251‑1 (MQ=255)                                                                                        gTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATCCCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTAc                                                                                                                                                                                 <  1:111330/219‑1 (MQ=255)                                                                                        gTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGTTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGc                                                                                                                                                                                                                                         <  1:349628/163‑1 (MQ=255)                                                                                        gTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTAc                                                                                                                                                                                 >  1:258237/1‑219 (MQ=255)                                                                                           aaGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGc                                                                                                                                              <  1:450906/250‑1 (MQ=255)                                                                                                    aTACGCTCAGTAGTAGTAGTTTTCCCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACgg                                                                                                                                       <  1:713545/249‑1 (MQ=255)                                                                                                    aTACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGTTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACgg                                                                                                                                       <  1:840835/249‑1 (MQ=255)                                                                                                    aTACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACGGATACCAAGGTGACGATATCTTTAAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACgg                                                                                                                                       <  1:149838/249‑1 (MQ=255)                                                                                                           cAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGa                                                                                                                                                                     >  1:285338/1‑212 (MQ=255)                                                                                                             gtagtagtagtTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCtt                                                                                                                             >  1:283072/1‑250 (MQ=255)                                                                                                                            cccGCATCAATATGCGCACTGATACAAATGTTACGATATCTTTCAAGAGGGTTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGc                                                                                                             <  1:414989/251‑1 (MQ=255)                                                                                                                               gCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATAc                                                                                                                                         <  1:586254/220‑1 (MQ=255)                                                                                                                                  tCAATATGCGCACTGATACCAATTTTACGTTATCTTTCAATAGGGTTTGAACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCg                                                                                                                                                                                         <  1:549388/169‑1 (MQ=255)                                                                                                                                    aaTATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATAc                                                                                                                                                                                               >  1:484526/1‑161 (MQ=255)                                                                                                                                            cACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCg                                                                                             >  1:623991/1‑251 (MQ=255)                                                                                                                                            cACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCg                                                                                             >  1:572809/1‑251 (MQ=255)                                                                                                                                                gATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATAc                                                                                                                                         >  1:97737/1‑203 (MQ=255)                                                                                                                                                  tACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGc                                                                                                                                              >  1:495693/1‑196 (MQ=255)                                                                                                                                                  tACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCg                                                                                       >  1:283689/1‑251 (MQ=255)                                                                                                                                                         gTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACa                                                                                >  1:330845/1‑251 (MQ=255)                                                                                                                                                                  tCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGc                                                                                                                                              >  1:403182/1‑180 (MQ=255)                                                                                                                                                                  tCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAg                                                                       >  1:156470/1‑251 (MQ=255)                                                                                                                                                                    tttCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACg                                                                                                                         <  1:457998/199‑1 (MQ=255)                                                                                                                                                                     ttCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTa                                                                    <  1:495197/251‑1 (MQ=255)                                                                                                                                                                       cAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTa                                                                                                                  <  1:598166/203‑1 (MQ=255)                                                                                                                                                                       cAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTaaa                                                                  >  1:679709/1‑251 (MQ=255)                                                                                                                                                                         aTAGTGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACg                                                                <  1:19812/251‑1 (MQ=255)                                                                                                                                                                             gggTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAg                                                                       <  1:348280/240‑1 (MQ=255)                                                                                                                                                                             gggTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTaaa                                                            >  1:205091/1‑251 (MQ=255)                                                                                                                                                                              ggTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAAc                                                                                                                >  1:876910/1‑198 (MQ=255)                                                                                                                                                                                 tGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCa                                                        <  1:91188/251‑1 (MQ=255)                                                                                                                                                                                  gTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCAt                                                       >  1:876284/1‑251 (MQ=255)                                                                                                                                                                                         ccATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATgaga                                                                                                                                                                                                                     <  1:793064/86‑1 (MQ=255)                                                                                                                                                                                         ccATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTa                                                >  1:698459/1‑251 (MQ=255)                                                                                                                                                                                           attattattTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTAt                                               >  1:710638/1‑250 (MQ=255)                                                                                                                                                                                            ttattattTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCa                                             >  1:34735/1‑251 (MQ=255)                                                                                                                                                                                                                  ttATTTAAAATTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCaa                       <  1:617324/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaTTAAATACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAAcc                                                                                                                                                                                          >  1:637007/1‑81 (MQ=255)                                                                                                                                                                                                                                aTACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATa         >  1:889273/1‑251 (MQ=255)                                                                                                                                                                                                                                 tACGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATAc        <  1:222671/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATACCt      <  1:525084/251‑1 (MQ=255)                                                                                                                                                                                                                                    gAAATTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATACCTa     >  1:91919/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaTTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCAc                                            >  1:259749/1‑211 (MQ=255)                                                                                                                                                                                                                                       aTTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAgc                               >  1:790417/1‑222 (MQ=255)                                                                                                                                                                                                                                       aTTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACg                     <  1:138787/232‑1 (MQ=255)                                                                                                                                                                                                                                       aTTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATACCTAATg  <  1:382720/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATACCTAATg  >  1:678322/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTTTTATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAAACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCg                                  >  1:443506/1‑219 (MQ=255)                                                                                                                                                                                                                                        ttttATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGttt                                                                                                >  1:358504/1‑156 (MQ=255)                                                                                                                                                                                                                                        ttttATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGtt                                                                         >  1:827787/1‑179 (MQ=255)                                                                                                                                                                                                                                          ttATATATGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACa                                                                                >  1:406738/1‑170 (MQ=255)                                                                                                                                                                                                                                            atatatGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACgg                                                                                                                                       <  1:150334/113‑1 (MQ=255)                                                                                                                                                                                                                                            atatatGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGa                                                                                           >  1:136636/1‑157 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ATGGTAATCCCACGTTCTTGTTCCTGTTCCATCCAGTCCATGGTTGCGGCACCATCGTGTACTTCACCGATTTTGTGACTTACACCGGTATAGAACAAGATACGCTCAGTAGTAGTAGTTTTACCCGCATCAATATGCGCACTGATACCAATGTTACGATATCTTTCAATAGGGGTTGTACGAGCCATTATTATTTCCTTGACGATTTCTTATTTAAAATTAAATACGAAATTTTATATACGAATGAGGCTTCACCGCATAGAAATGAGATGAAGCCCAAAAACGTAAATACTAACCGAATATTACCAACGGTAGTGAGCAAACGCTTTGTTAGCTTCAGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCCGCGCCTTTGTTTTCGGAAGCGTCAGACAATTCGTTAGCTAAACGTAAAGCCATGGATTTATCACCGCGTTTGCGAGCTGCTTCAACGATCCAACGCATACCTAATG  >  1/375353‑375833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑