Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,860,502	A→G	intergenic (+65575/‑73829)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,860,502	0	A	G	100.0%	99.3 / NA	36	intergenic (+65575/‑73829)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

ATTACAAACTTCTTCAATATCAATACTATAAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTATTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAGCTTCGATTTTGCTTAAATATCATGTGATTTTAACAAAACCGATCGTAAATTATCTACTCAGATACGAATTTATAACTATCAAAA  >  1/1860282‑1860747                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      aTTACAAACTTCTTCAATATCAATACTATAAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACttttt                                                                                                                                                                                                                         >  1:657398/1‑251 (MQ=255)  ttACAAACTTCTTCAATATCAATACTATAAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACtttttt                                                                                                                                                                                                                        >  1:441903/1‑251 (MQ=255)         cttcttCAATATCAATACTATAAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGAt                                                                                                                                                                                                                  >  1:70583/1‑250 (MQ=255)                          cTATAAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTAT‑AACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATc                                                                                                                                                                                                >  1:697352/1‑250 (MQ=255)                           tataAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATcc                                                                                                                                                                                               <  1:141912/251‑1 (MQ=255)                            ataAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATccc                                                                                                                                                                                              <  1:594036/251‑1 (MQ=255)                            ataAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATcc                                                                                                                                                                                               >  1:225779/1‑250 (MQ=255)                                    cACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAAc                                                                                                                                                                                      <  1:74806/251‑1 (MQ=255)                                      cTCGGTTTGTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACta                                                                                                                                                                                    <  1:59380/251‑1 (MQ=255)                                            ttttCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTAttatta                                                                                                                                                                              <  1:304888/251‑1 (MQ=255)                                             tttCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTAc                                                                                                                                                                             <  1:224932/251‑1 (MQ=255)                                             tttCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTAc                                                                                                                                                                             <  1:581258/251‑1 (MQ=255)                                              ttCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACt                                                                                                                                                                            <  1:503298/251‑1 (MQ=255)                                                 aataatGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCAACTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTtat                                                                                                                                                                         <  1:400578/251‑1 (MQ=255)                                                     atGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGAt                                                                                                                                                                     <  1:122838/251‑1 (MQ=255)                                                                  gCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAAc                                                                                                                                                                                      >  1:680656/1‑221 (MQ=255)                                                                                     aTCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTAttatta                                                                                                                                                                              >  1:642162/1‑210 (MQ=255)                                                                                       ccTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAg                                                                                                                                                    <  1:302751/234‑1 (MQ=255)                                                                                                             catcatTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAAc                                                                                                                                                                                      <  1:105614/178‑1 (MQ=255)                                                                                                                catTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTtg                                                                                                                                                                                                                      >  1:641136/1‑143 (MQ=255)                                                                                                                catTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAAc                                                                                                          <  1:288747/251‑1 (MQ=255)                                                                                                                     ccGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTAttatta                                                                                                                                                                              >  1:704125/1‑178 (MQ=255)                                                                                                                            ggAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAg                                                                                                                                                             <  1:313320/188‑1 (MQ=255)                                                                                                                             gAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAAc                                                                                                                     <  1:784261/227‑1 (MQ=255)                                                                                                                                 tatCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAAc                                                                                                                     <  1:832594/223‑1 (MQ=255)                                                                                                                                   tCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTAccc                                                                                                                                         <  1:20889/201‑1 (MQ=255)                                                                                                                                     aTTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAGAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAg                                                                                      >  1:899099/1‑250 (MQ=255)                                                                                                                                      ttATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTAccc                                                                                                                                         <  1:263931/198‑1 (MQ=255)                                                                                                                                      ttATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCattta                                                                                           <  1:539641/244‑1 (MQ=255)                                                                                                                                                            ccTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAGCTTCGATTTTGCTTAAATATCAtg                                                              >  1:365517/1‑251 (MQ=255)                                                                                                                                                               ctTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTAc                                                                                                                                                                             >  1:750282/1‑137 (MQ=255)                                                                                                                                                                                 gTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAgg                                                                                                                                                   >  1:864383/1‑145 (MQ=255)                                                                                                                                                                                           ggTGATGATTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAAc                                                                                                          >  1:558116/1‑176 (MQ=255)                                                                                                                                                                                               atgatTGTGTTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAGCTTCGATTTTGCTTAAATATCATGTGATTTTAACAAAACCGATCGTAAATTATCTACTc                           <  1:757498/251‑1 (MQ=255)                                                                                                                                                                                                      tgtTTTGATTTTGTTTCTCATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAGCTTCGATTTTGCTTAAATATCATGTGATTTTAACAAAACCGATCGTAAATTATCTACTCAGATAc                     <  1:534560/250‑1 (MQ=255)                                                                                                                                                                                                                        cATTTGTTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAGCTTCGATTTTGCTTAAATATCATGTGATTTTAACAAAACCGATCGTAAATTATCTACTCAGATACGAATTTATAACTATCaaaa  >  1:811957/1‑251 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      ATTACAAACTTCTTCAATATCAATACTATAAATATCACTCGGTTTTTCAATAATGCCTTCCGATAGCGAAAATCCCATAGCGAAATCCTCTAAATCACGGGGCGAACACATCATTACCGTATGGGAAATATCATTATAAACCAGCGCCACAGGCACCTCTTTTGCGAGGGTTTCTTGTTTCTCGGAGGTGATGATTGTGTTTTGATTTTGTTTCTCATTTATTAATTTTTTGATAAAAAAAATTACTTTTTTTGTGATCCAGTTCAAAATTTTATCCCATTATAACTATTATTACTTATAGATTTCCGTAGAAAAAATAGGTATTCTACCCACCGCTAACAAATGTATAACAGATATTTAACATATCACATCATTTATTTAGCTTCGATTTTGCTTAAATATCATGTGATTTTAACAAAACCGATCGTAAATTATCTACTCAGATACGAATTTATAACTATCAAAA  >  1/1860282‑1860747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑