Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,857,446	T→C	intergenic (+62519/‑76885)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,857,446	0	T	C	100.0%	190.3 / NA	72	intergenic (+62519/‑76885)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/38);  total (34/38)

ATTTTGCACGGAGGTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACTGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCC  >  1/1857206‑1857689                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aTTTTGCACGGAGGTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCAt                                                                                                                                                                                                                                            >  1:497710/1‑251 (MQ=255)       cACGGAGGTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGc                                                                                                                                                                                                                                       <  1:364201/250‑1 (MQ=255)           gAGGTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGa                                                                                                                                                                                                                                   >  1:688252/1‑250 (MQ=255)            aGGTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGAc                                                                                                                                                                                                                                  <  1:612541/250‑1 (MQ=255)              gTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGa                                                                                                                                                                                                                               >  1:387776/1‑251 (MQ=255)                                 tgtTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGc                                                                                                                                                                                                             <  1:371374/250‑1 (MQ=255)                                 tgtTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCg                                                                                                                                                                                                            <  1:833792/251‑1 (MQ=255)                                                       ttCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCa                                                                                                                                                                                         <  1:836550/248‑1 (MQ=255)                                                           acaAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGac                                                                                                                                                                                  <  1:661103/251‑1 (MQ=255)                                                                          cTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCAt                                                                                                                                                                    >  1:846813/1‑250 (MQ=255)                                                                            gATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAGGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGaccacc                                                                                                                                                                              <  1:884244/238‑1 (MQ=255)                                                                            gATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGaccacc                                                                                                                                                                              <  1:107689/238‑1 (MQ=255)                                                                            gATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCaa                                                                                                                                                                 >  1:463352/1‑251 (MQ=255)                                                                            gATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGCAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCCAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAAACTGACCCCCACAACGGCATCaa                                                                                                                                                                 >  1:195441/1‑251 (MQ=255)                                                                              tGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCg                                                                                                                                                                                                                        <  1:794097/194‑1 (MQ=255)                                                                              tGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGAc                                                                                                                                                                                                 >  1:592357/1‑217 (MQ=255)                                                                              tGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAg                                                                                                                                                               >  1:309462/1‑251 (MQ=255)                                                                              tGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAg                                                                                                                                                               >  1:106516/1‑251 (MQ=255)                                                                              tGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCAACGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGGTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAg                                                                                                                                                               >  1:88883/1‑251 (MQ=255)                                                                              tGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATAATCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTAGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAg                                                                                                                                                               <  1:513180/251‑1 (MQ=255)                                                                                   aTACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGAAAGGCAACATGACCACCAGACCGGCATCAAAGaaaaa                                                                                                                                                          >  1:348137/1‑250 (MQ=255)                                                                                          aCAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAg                                                                                                                                                  >  1:522759/1‑251 (MQ=255)                                                                                             aaTAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCAt                                                                                                                                                                    >  1:699523/1‑231 (MQ=255)                                                                                             aaTAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCg                                                                                                                                               >  1:627809/1‑251 (MQ=255)                                                                                                  gTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGa                                                                                                                                                                                                                               <  1:847261/167‑1 (MQ=255)                                                                                                  gTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAg                                                                                                                                          >  1:544247/1‑251 (MQ=255)                                                                                                  gTACCGACGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGaaa                                                                                                                                                                                                                     <  1:446388/177‑1 (MQ=255)                                                                                                     ccGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCaa                                                                                                                                       <  1:553432/251‑1 (MQ=255)                                                                                                                ggTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGc                                                                                                                                                                                          >  1:6635/1‑190 (MQ=255)                                                                                                                ggTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATc                                                                                                                             >  1:482145/1‑250 (MQ=255)                                                                                                                ggTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATc                                                                                                                             >  1:758541/1‑250 (MQ=255)                                                                                                                       aTCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAgcgc                                                                                                                     <  1:900411/251‑1 (MQ=255)                                                                                                                             cAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAgg                                                                                                               <  1:885050/251‑1 (MQ=255)                                                                                                                               aaTTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATcccc                                                                                                                          <  1:174725/238‑1 (MQ=255)                                                                                                                               aaTTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGgcg                                                                                                             >  1:8776/1‑251 (MQ=255)                                                                                                                                           aaTCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAgcgc                                                                                                                     <  1:17104/231‑1 (MQ=255)                                                                                                                                            aTCACCATCAAGCGCATATTCACGCCGATTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACACGTGCATGACCGCAAAACCACCCTCAACCGGGGAGGCATAACGCAAGGCAGTACCACCGCAAGGTTTTGCGGCAATAAAGACGAAAGGCAACACGGCCACCAGACCGGCAGCAAAGAAAATGCGGG‑ATACGAACAACAACACGGTAAACCGCATCTCAAAAGACGCCTTCTTTTCGc                                                                                                 >  1:550626/1‑250 (MQ=255)                                                                                                                                            aTCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACAATAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGc                                                                                                 >  1:57821/1‑250 (MQ=255)                                                                                                                                                      aGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAt                                                                                                                                                          <  1:695467/184‑1 (MQ=255)                                                                                                                                                       gCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGAt                                                                                                                                                                                              >  1:177962/1‑147 (MQ=255)                                                                                                                                                        cccATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTaa                                                                                     <  1:739098/250‑1 (MQ=255)                                                                                                                                                        cccATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTaa                                                                                     <  1:251736/250‑1 (MQ=255)                                                                                                                                                        cccATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAAt                                                                                    >  1:755398/1‑251 (MQ=255)                                                                                                                                                           atatTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAg                                                                                                                >  1:620120/1‑220 (MQ=255)                                                                                                                                                            tatTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATcccc                                                                                                                          <  1:644884/209‑1 (MQ=255)                                                                                                                                                            tatTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCaa                                                                                 >  1:745200/1‑250 (MQ=255)                                                                                                                                                            tatTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCaa                                                                                 >  1:76148/1‑250 (MQ=255)                                                                                                                                                              ttCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGt                                                                              <  1:315498/251‑1 (MQ=255)                                                                                                                                                              ttCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAACGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGt                                                                              >  1:57966/1‑251 (MQ=255)                                                                                                                                                                 ccGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATc                                                                           <  1:565643/250‑1 (MQ=255)                                                                                                                                                                    ccGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCg                                                                         <  1:99264/250‑1 (MQ=255)                                                                                                                                                                      gAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTTAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACCAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CAGGAAGCCGAACAGCAACGCGGCAATCCCCAACGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCaa                                                                      >  1:429149/1‑251 (MQ=255)                                                                                                                                                                      gAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGa                                                                                                                                                                                   <  1:24185/143‑1 (MQ=255)                                                                                                                                                                          aaaTCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCAc                                                                  <  1:724483/251‑1 (MQ=255)                                                                                                                                                                                        cACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGctttc                                                                                                        >  1:495380/1‑199 (MQ=255)                                                                                                                                                                                             gACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGc                                                            >  1:396707/1‑238 (MQ=255)                                                                                                                                                                                             gACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGa                                                 >  1:262035/1‑249 (MQ=255)                                                                                                                                                                                                    aTGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGAAAGGCATAACGCAAGACGGAACCACCAAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAACCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATa                                        <  1:712394/251‑1 (MQ=255)                                                                                                                                                                                                            gTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATcccc                                                                                                                          <  1:165455/161‑1 (MQ=255)                                                                                                                                                                                                            gTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGt                                                                              <  1:421972/205‑1 (MQ=255)                                                                                                                                                                                                              aaaacCGCGTGCATGACCCCAAAAGCCCCGACAACCGGAAAGGCATAACCCAAGACGAACCCACCAAAACGTTTTCCGAGACTAAGAACAAAGGGAAACATGCCCACCAGCCCGGCATAAAAAAAAAT‑CGGGAAGCAAAACATCAACGCGCCAAGCCCCAGCGCAAAAGTCGCTTTCTTTTCGCCGAATTTATAAATCAACGTACCCGCAAGCTCTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGAt                              <  1:580417/251‑1 (MQ=255)                                                                                                                                                                                                                 aaCGCGTGCATGACCGCAAAGGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATAAAAGAAAAC‑CGGGAAGCCGAACAGCAACGCGGCAACCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATAAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCAt                           <  1:366325/251‑1 (MQ=255)                                                                                                                                                                                                                      gTGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTGTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATaaa                                      <  1:443288/235‑1 (MQ=255)                                                                                                                                                                                                                       tGCATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGACCCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAA‑TTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTAccc                    <  1:501429/251‑1 (MQ=255)                                                                                                                                                                                                                         cATGACCGCAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTAccc                    <  1:476762/250‑1 (MQ=255)                                                                                                                                                                                                                             aCCGCAAAGGCACCGGCAACCGGGAAGGCATAACGCAAGACGGACCCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGCCCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAAc                <  1:826992/250‑1 (MQ=255)                                                                                                                                                                                                                                 cAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCa                                                                       >  1:173204/1‑191 (MQ=255)                                                                                                                                                                                                                                 cAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGa             >  1:823170/1‑249 (MQ=255)                                                                                                                                                                                                                                 cAAAAGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGa             >  1:11219/1‑249 (MQ=255)                                                                                                                                                                                                                                    aaGCACCGGCAACCGGGAAGGCATAACGCAAGACGGAACCCCCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACtaat        <  1:698103/251‑1 (MQ=255)                                                                                                                                                                                                                                       cACCGGCACCCGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAAc     <  1:156087/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGGCAACCGGGAAGGCATAACGCAAGACGGACCCCCCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGcc  <  1:545012/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     ATTTTGCACGGAGGTTTCGCTGATGGCGGCGGTGTTCAAAAAGGTTTTCGGCAGTTCCACAAAAAATTTTTTACTGATGTACATACTGAACAAATAAGTACCGATGTACCAGGTCGGGATCGCGCAAATTAAGCCAACAATCACCATCAAGCCCATATTCACGCCGAGTAAATCACCGGAAGCCACCGGACCCGGATGCGGCGGTAAAAACGCGTGCATGACCGCAAAAGCACCGGCAACTGGGAAGGCATAACGCAAGACGGAACCACCGAAACGTTTTGCGACACTAAAGACGATAGGCAACATGACCACCAGACCGGCATCAAAGAAAAT‑CGGGAAGCCGAACAGCAACGCGGCAATCCCCAGCGCAAAAGGCGCTTTCTTTTCGCCGAATTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCC  >  1/1857206‑1857689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑