Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,855,806	C→T	intergenic (+60879/‑78525)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,855,806	0	C	T	100.0%	202.9 / NA	74	intergenic (+60879/‑78525)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/34);  total (40/34)

GCCGTAAATAGACTGGTCGTCATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCCTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATGGGCGCGTAGCAAT  >  1/1855575‑1856052                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gCCGTAAATAGACTGGTCGTCATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTgataaatttgataaattt                                                                                                                                                                                                                                     >  1:580216/1‑251 (MQ=255)    gTAAATAGACTGGTCGTCATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGa                                                                                                                                                                                                                                  >  1:439686/1‑251 (MQ=255)     tAAATAGACTGGTCGTCATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGAt                                                                                                                                                                                                                                 >  1:866781/1‑251 (MQ=255)      aaaTAGACTGGTCGTCATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATc                                                                                                                                                                                                                                <  1:67797/251‑1 (MQ=255)                 tcgtcATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTCGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTc                                                                                                                                                                                                                     <  1:433823/251‑1 (MQ=255)                              caATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTtg                                                                                                                                                                                                        >  1:298061/1‑251 (MQ=255)                              caATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTtg                                                                                                                                                                                                        >  1:343983/1‑251 (MQ=255)                              caATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTtg                                                                                                                                                                                                        >  1:551920/1‑251 (MQ=255)                              caATCATCACTTTGCCGGTTTTGACCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTtg                                                                                                                                                                                                        <  1:773167/251‑1 (MQ=255)                               aaTCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTtgt                                                                                                                                                                                                       >  1:622200/1‑251 (MQ=255)                                atcatcACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACg                                                                                                                                                                                                                        <  1:526776/233‑1 (MQ=255)                                atcatcACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTtgtg                                                                                                                                                                                                      >  1:754991/1‑251 (MQ=255)                                      aCTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGATGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGt                                                                                                                                                                                                 >  1:864374/1‑250 (MQ=255)                                       cTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGtt                                                                                                                                                                                                                      <  1:286230/228‑1 (MQ=255)                                         ttGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTtgataaa                                                                                                                                                                                                                                                 >  1:157192/1‑199 (MQ=255)                                            ccGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAAtgt                                                                                                                                                                                           <  1:850305/250‑1 (MQ=255)                                                      ccGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGc                                                                                                                                                                                <  1:12166/251‑1 (MQ=255)                                                         ccAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGtt                                                                                                                                                                                                                      <  1:26812/210‑1 (MQ=255)                                                         ccAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAAGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAg                                                                                                                                                                              >  1:446238/1‑250 (MQ=255)                                                              aaGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTc                                                                                                                                                                        <  1:534263/251‑1 (MQ=255)                                                                   aaTCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCAtct                                                                                                                                                                    <  1:725270/250‑1 (MQ=255)                                                                   aaTCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGCAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCAtctt                                                                                                                                                                   >  1:172719/1‑251 (MQ=255)                                                                          tCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCttatt                                                                                                                                                            <  1:336616/251‑1 (MQ=255)                                                                            atatTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTtgat                                                                                                                                                                                                                                                    >  1:518779/1‑161 (MQ=255)                                                                            atatTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTAGGACGCAGcc                                                                                                                                                                            >  1:235841/1‑233 (MQ=255)                                                                                 gAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCAtc                                                                                                                                                                     <  1:481207/235‑1 (MQ=255)                                                                                  aaTTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATg                                                                                                                                                     <  1:218697/250‑1 (MQ=255)                                                                                  aaTTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATg                                                                                                                                                     <  1:144801/250‑1 (MQ=255)                                                                                   aTTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGAc                                                                                                                                                                                  <  1:555483/220‑1 (MQ=255)                                                                                        gttgGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAAtgt                                                                                                                                                                                           >  1:337235/1‑206 (MQ=255)                                                                                         ttgGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAAc                                                                                                                                              <  1:478598/250‑1 (MQ=255)                                                                                              aTCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGa                                                                                                                                                                                                     >  1:540406/1‑190 (MQ=255)                                                                                              aTCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAAc                                                                                                                                              <  1:542337/245‑1 (MQ=255)                                                                                               tCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAACTCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCa                                                                                                                                                >  1:652028/1‑242 (MQ=255)                                                                                                cTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCAt                                                                                                                                                                      >  1:679655/1‑219 (MQ=255)                                                                                                     aaCTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCAtc                                                                                                                                                                     >  1:3470/1‑215 (MQ=255)                                                                                                                        gCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCTCCAACCCTGCACGGTCGCAGGCATCTTAATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGa                                                                                                                                                       <  1:179428/210‑1 (MQ=255)                                                                                                                         cTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAg                                                                                                                                            <  1:109537/220‑1 (MQ=255)                                                                                                                                cGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAAC‑TTTTTCGTCGAAATtgtg                                                                                                      >  1:286214/1‑250 (MQ=255)                                                                                                                                    gttgATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTtgat                                                                                                                                                                                                                                                    <  1:423882/105‑1 (MQ=255)                                                                                                                                    gttgATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATc                                                                                                                                                                                                                                <  1:547209/125‑1 (MQ=255)                                                                                                                                    gttgATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGAt                                                                                                                                                                                                    >  1:287729/1‑153 (MQ=255)                                                                                                                                    gttgATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCttatta                                                                                                                                                           <  1:680820/194‑1 (MQ=255)                                                                                                                                    gttgATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGt                                                                                                  >  1:36817/1‑251 (MQ=255)                                                                                                                                       gATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTAc                                                                                                                                                  >  1:85863/1‑200 (MQ=255)                                                                                                                                           aCGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCGACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTa                                                                                           <  1:28633/251‑1 (MQ=255)                                                                                                                                           aCGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTa                                                                                           <  1:394166/251‑1 (MQ=255)                                                                                                                                               tGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCg                                                                                       <  1:739441/251‑1 (MQ=255)                                                                                                                                                      tCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGc                                                                                                                                          >  1:288568/1‑193 (MQ=255)                                                                                                                                                                gtgtAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAAtg                                                                                                                                                                                            >  1:482436/1‑133 (MQ=255)                                                                                                                                                                gtgtAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCaa                                                                        >  1:59417/1‑249 (MQ=255)                                                                                                                                                                  gtAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGAt                                                                                 >  1:232767/1‑238 (MQ=255)                                                                                                                                                                   tAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGa                                                                    <  1:652447/250‑1 (MQ=255)                                                                                                                                                                       aaCAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTc                                                               <  1:763276/251‑1 (MQ=255)                                                                                                                                                                              cGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATc                                                         >  1:312756/1‑250 (MQ=255)                                                                                                                                                                               gTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCa                                                       >  1:202280/1‑251 (MQ=255)                                                                                                                                                                                        taataaAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCGAAATCTGGa                                              >  1:629586/1‑251 (MQ=255)                                                                                                                                                                                                        aaaTCCACCAACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGGGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATc                              <  1:430373/251‑1 (MQ=255)                                                                                                                                                                                                             caccaACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGAGATCGTCAATGTGATTCGGACGCAGCCCTTCAtctttc                                                                                                                                                                 >  1:69359/1‑115 (MQ=255)                                                                                                                                                                                                              accaACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATc                        <  1:813899/251‑1 (MQ=255)                                                                                                                                                                                                              accaACCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATc                        <  1:604028/251‑1 (MQ=255)                                                                                                                                                                                                                  aCCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGc                                   <  1:777913/236‑1 (MQ=255)                                                                                                                                                                                                                  aCCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAgg                    >  1:225901/1‑251 (MQ=255)                                                                                                                                                                                                                  aCCCTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAgg                    >  1:789877/1‑251 (MQ=255)                                                                                                                                                                                                                   cccTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCt                                         <  1:28343/229‑1 (MQ=255)                                                                                                                                                                                                                   cccTGCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGt                   >  1:489617/1‑251 (MQ=255)                                                                                                                                                                                                                       gCACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATg               >  1:895530/1‑251 (MQ=255)                                                                                                                                                                                                                        cACGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATg               <  1:858436/250‑1 (MQ=255)                                                                                                                                                                                                                         aCGATCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATggg             <  1:180755/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATGGgcg           >  1:67798/1‑250 (MQ=255)                                                                                                                                                                                                                            aTCGCAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATGGgcg           >  1:280179/1‑250 (MQ=255)                                                                                                                                                                                                                                cAGGCATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATGGGCGCGTAg      >  1:838981/1‑251 (MQ=255)                                                                                                                                                                                                                                    cATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCAt                         >  1:758472/1‑228 (MQ=255)                                                                                                                                                                                                                                    cATCTTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATGGGCGCGTAGCAAt  >  1:2166/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GCCGTAAATAGACTGGTCGTCATCGCCCACAATCATCACTTTGCCGGTTTTGCCCGCCAGCAAGCGAATCCATTCATATTGAATTTTGTTGGTATCTTGGAACTCATCCACCAAAATATGCTGAAAACGTTGTTGATAACGTTGCAAAATCAGCGGTTTGTGTAAAAACAGTTCGTAAGAACGTAATAAAATTTCGGCAAAATCCACCAACCCTGCACGATCGCAGGCATCCTGATAAATTTGATAAATTTTGATCCATTCACGTTCCTGACGATCGTTGTGATCGTCAATGTGATTCGGACGCAGCCCTTCATCTTTCTTATTATTGATGTACCAACAGGCTTGTTTCGGTGGAAAACTTTTTTCGTCGAAATTGTGCAGTTTCATTAAGCGTTTGATTAAACGCAACTGATCTTCCGAATCCAAAATCTGGAAATCTTGCGGCAGATCCGCATCAAGGTGATGGGCGCGTAGCAAT  >  1/1855575‑1856052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑