Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,854,828	T→C	intergenic (+59901/‑79503)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,854,828	0	T	C	100.0%	205.7 / NA	73	intergenic (+59901/‑79503)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/37);  total (36/37)

AACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCACGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGTACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCGGCGCGCCCGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTGCGCAAGGTATCCAAC  >  1/1854587‑1855074                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       aaCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCGTCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTcccc                                                                                                                                                                                                                                                <  1:194663/250‑1 (MQ=255)  aCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCtt                                                                                                                                                                                                                                              <  1:344721/251‑1 (MQ=255)          tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                                                                                                                                                                                      <  1:577202/251‑1 (MQ=255)          tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                                                                                                                                                                                      <  1:753392/251‑1 (MQ=255)                    tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGtt                                                                                                                                                                                                                            <  1:848241/251‑1 (MQ=255)                              ccGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTg                                                                                                                                                                                                                  <  1:173154/250‑1 (MQ=255)                                  cAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                                                                                              <  1:853839/251‑1 (MQ=255)                                       aTGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                                                                                                                                                                         <  1:792026/251‑1 (MQ=255)                                            aaCACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAAt                                                                                                                                                                                                    <  1:769372/251‑1 (MQ=255)                                                     aaaCTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATca                                                                                                                                                                                           <  1:730571/251‑1 (MQ=255)                                                        cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                                                                                                                                                                        >  1:21712/1‑251 (MQ=255)                                                        cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                                                                                                                                                                        >  1:702108/1‑251 (MQ=255)                                                            aaGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATc                                                                                                                                                                                    <  1:314693/251‑1 (MQ=255)                                                              gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                                                                                              >  1:632251/1‑223 (MQ=255)                                                              gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                                                                                                                                                                         >  1:622001/1‑228 (MQ=255)                                                              gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaaaa                                                                                                                                                                                      <  1:121768/247‑1 (MQ=255)                                                              gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCg                                                                                                                                                                                  >  1:539257/1‑251 (MQ=255)                                                                     aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTa                                                                                                                                                                                               >  1:577582/1‑231 (MQ=255)                                                                     aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                                                                                                                                                                            >  1:660590/1‑250 (MQ=255)                                                                     aCCGAATGCAGCATCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGCCAGATCCGACATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                                                                                                                                                                            >  1:78286/1‑250 (MQ=255)                                                                               gCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                                                                                              <  1:699673/206‑1 (MQ=255)                                                                                       atTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc                                                                                                                                                         <  1:281614/251‑1 (MQ=255)                                                                                        tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc                                                                                                                                                         >  1:235853/1‑250 (MQ=255)                                                                                        tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc                                                                                                                                                         >  1:792460/1‑250 (MQ=255)                                                                                                  aGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCg                                                                                                                                                >  1:780508/1‑249 (MQ=255)                                                                                                  aGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCg                                                                                                                                                >  1:465670/1‑249 (MQ=255)                                                                                                   gCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCg                                                                                                                                                >  1:224412/1‑248 (MQ=255)                                                                                                       gATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCaaa                                                                                                                                         >  1:28706/1‑251 (MQ=255)                                                                                                            ggCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGaatt                                                                                                                                    >  1:360832/1‑251 (MQ=255)                                                                                                                  gCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCgtgt                                                                                                                                                    >  1:863444/1‑229 (MQ=255)                                                                                                                   ccTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGaattaatt                                                                                                                                <  1:119144/248‑1 (MQ=255)                                                                                                                          ctcGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATaa                                                                                                                      <  1:410702/251‑1 (MQ=255)                                                                                                                            cgcccgccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAg                                                                                                                    <  1:899684/251‑1 (MQ=255)                                                                                                                            cgcccgccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTCTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAg                                                                                                                    <  1:376517/251‑1 (MQ=255)                                                                                                                              cccgccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACCCCATAAAGCg                                                                                                                  <  1:892089/251‑1 (MQ=255)                                                                                                                                 gccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGaa                                                                                                                                                                                                     >  1:767417/1‑165 (MQ=255)                                                                                                                                  ccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTAc                                                                                                                                                                                                                       <  1:870549/146‑1 (MQ=255)                                                                                                                                  ccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                                                                                              <  1:169768/155‑1 (MQ=255)                                                                                                                                  ccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTaa                                                                                                               >  1:156473/1‑250 (MQ=255)                                                                                                                                     ccAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGtt                                                                                                                                                                                <  1:477425/182‑1 (MQ=255)                                                                                                                                     ccAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAAt                                                                                                           >  1:371483/1‑251 (MQ=255)                                                                                                                                                tGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcag                                                                                                 <  1:331277/250‑3 (MQ=255)                                                                                                                                                tGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcag                                                                                                 <  1:531213/250‑3 (MQ=255)                                                                                                                                                 gAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcagcg                                                                                               >  1:755563/1‑251 (MQ=255)                                                                                                                                                  aGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcagcg                                                                                               >  1:403211/1‑250 (MQ=255)                                                                                                                                                     tAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGa                                                                                                                                       <  1:541663/207‑1 (MQ=255)                                                                                                                                                              cGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAgtt                                                                                  >  1:556795/1‑251 (MQ=255)                                                                                                                                                                   gATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAgtttgttt                                                                             >  1:770728/1‑251 (MQ=255)                                                                                                                                                                     tCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGtttt                                                                            >  1:709317/1‑250 (MQ=255)                                                                                                                                                                      ccGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTc                                                                           >  1:482220/1‑250 (MQ=255)                                                                                                                                                                        gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACAtt                                                                                                                                                                                                                    >  1:617908/1‑111 (MQ=255)                                                                                                                                                                        gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                                                                                                                                                                         <  1:871816/122‑1 (MQ=255)                                                                                                                                                                        gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGc                                                                                                                   >  1:74681/1‑208 (MQ=255)                                                                                                                                                                        gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTc                                                                           >  1:543343/1‑248 (MQ=255)                                                                                                                                                                        gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTc                                                                           >  1:806905/1‑248 (MQ=255)                                                                                                                                                                                       tCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcag                                                                                                 >  1:231382/1‑209 (MQ=255)                                                                                                                                                                                       tCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGa                                                              <  1:205527/246‑1 (MQ=255)                                                                                                                                                                                       tCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATtggt                                                         >  1:847941/1‑251 (MQ=255)                                                                                                                                                                                        cTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgc                                                                                                   >  1:722054/1‑208 (MQ=255)                                                                                                                                                                                                     aaaTTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAgg                                           <  1:594602/251‑1 (MQ=255)                                                                                                                                                                                                       aTTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATaa                                                                                                                      <  1:82843/174‑1 (MQ=255)                                                                                                                                                                                                       aTTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGc                                                                                                         <  1:357180/187‑1 (MQ=255)                                                                                                                                                                                                              ggcggcAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGAt                                  >  1:753859/1‑251 (MQ=255)                                                                                                                                                                                                                  gcAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGtt                               >  1:40844/1‑250 (MQ=255)                                                                                                                                                                                                                       cacCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATtggtgg                                                       <  1:129479/221‑1 (MQ=255)                                                                                                                                                                                                                                  ccAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTgcgc              <  1:427804/251‑1 (MQ=255)                                                                                                                                                                                                                                        cttCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAgcg                                                                   >  1:42326/2‑192 (MQ=255)                                                                                                                                                                                                                                           cAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATg                                 <  1:835388/223‑1 (MQ=255)                                                                                                                                                                                                                                           cAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTGCGCAAGGTATcc     <  1:460408/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAAc                                                                                                              <  1:322642/144‑1 (MQ=255)                                                                                                                                                                                                                                             aTACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTGCGCAAGGTATCCaa   >  1:353191/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTGCGCAAGGTATCCaa   <  1:183427/251‑1 (MQ=255)                                                                                                                                                                                                                                              tACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTGCGCAAGGTATCCAAc  <  1:630018/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       AACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCACGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGTACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCGGCGCGCCCGGCGAGTTTGTTTTCCTGAAGCGCCAGATTGGTGGCTTGCCATAAGGTGATTTGATGTTCACGGGTAAGATTGCGCAAGGTATCCAAC  >  1/1854587‑1855074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑