Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,854,766	A→G	intergenic (+59839/‑79565)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,854,766	0	A	G	100.0%	192.6 / NA	71	intergenic (+59839/‑79565)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/32);  total (39/32)

GTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCACGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGTACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCGGCGCGCCCGGCGAGTTTGTTTTC  >  1/1854529‑1855001                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            gTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                                                                                                 <  1:95355/250‑1 (MQ=255) gTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                                                                                                 <  1:880498/250‑1 (MQ=255)          aCGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAAtt                                                                                                                                                                                                                        >  1:20983/1‑250 (MQ=255)          aCGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTc                                                                                                                                                                                                                       <  1:894966/251‑1 (MQ=255)             tATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACg                                                                                                                                                                                                                    >  1:532592/1‑251 (MQ=255)                           ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACAcc                                                                                                                                                                                                       <  1:852175/250‑1 (MQ=255)                           ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCa                                                                                                                                                                                                      >  1:227467/1‑251 (MQ=255)                           ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATAAAGCGCGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCa                                                                                                                                                                                                      >  1:434712/1‑251 (MQ=255)                                       aCGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGt                                                                                                                                                                                          <  1:571353/251‑1 (MQ=255)                                                  cttcAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAGATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACg                                                                                                                                                                                >  1:232659/1‑250 (MQ=255)                                                           aaCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCGTCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTcccc                                                                                                                                                                       <  1:194663/250‑1 (MQ=255)                                                            aCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCtt                                                                                                                                                                     <  1:344721/251‑1 (MQ=255)                                                                    tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                                                                                                             <  1:577202/251‑1 (MQ=255)                                                                    tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                                                                                                             <  1:753392/251‑1 (MQ=255)                                                                     cGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCaa                                                                                                                                                                                    >  1:396035/1‑227 (MQ=255)                                                                              tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAAttc                                                                                                                                                                                                  >  1:388727/1‑204 (MQ=255)                                                                              tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGtt                                                                                                                                                   <  1:848241/251‑1 (MQ=255)                                                                                    ttcCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCTGACACCAAttc                                                                                                                                                                                                  <  1:235705/198‑1 (MQ=255)                                                                                        ccGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTg                                                                                                                                         <  1:173154/250‑1 (MQ=255)                                                                                            cAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                     <  1:853839/251‑1 (MQ=255)                                                                                             aaTCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                                                                                                 >  1:703286/1‑158 (MQ=255)                                                                                                 aTGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                                                                                                <  1:792026/251‑1 (MQ=255)                                                                                                      aaCACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAAt                                                                                                                           <  1:769372/251‑1 (MQ=255)                                                                                                               aaaCTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATca                                                                                                                  <  1:730571/251‑1 (MQ=255)                                                                                                                  cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                                                                                               >  1:702108/1‑251 (MQ=255)                                                                                                                  cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                                                                                               >  1:21712/1‑251 (MQ=255)                                                                                                                      aaGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATc                                                                                                           <  1:314693/251‑1 (MQ=255)                                                                                                                        gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                     >  1:632251/1‑223 (MQ=255)                                                                                                                        gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                                                                                                >  1:622001/1‑228 (MQ=255)                                                                                                                        gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaaaa                                                                                                             <  1:121768/247‑1 (MQ=255)                                                                                                                        gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCg                                                                                                         >  1:539257/1‑251 (MQ=255)                                                                                                                               aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTa                                                                                                                      >  1:577582/1‑231 (MQ=255)                                                                                                                               aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                                                                                                   >  1:660590/1‑250 (MQ=255)                                                                                                                               aCCGAATGCAGCATCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGCCAGATCCGACATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                                                                                                   >  1:78286/1‑250 (MQ=255)                                                                                                                                         gCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                     <  1:699673/206‑1 (MQ=255)                                                                                                                                           gTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTc                                                                                                                                                                                                                                         >  1:502665/1‑104 (MQ=255)                                                                                                                                                 atTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc                                                                                <  1:281614/251‑1 (MQ=255)                                                                                                                                                  tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc                                                                                >  1:792460/1‑250 (MQ=255)                                                                                                                                                  tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc                                                                                >  1:235853/1‑250 (MQ=255)                                                                                                                                                            aGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACgc                                                                                                                                                                               >  1:306674/1‑144 (MQ=255)                                                                                                                                                            aGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCg                                                                       >  1:780508/1‑249 (MQ=255)                                                                                                                                                            aGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCg                                                                       >  1:465670/1‑249 (MQ=255)                                                                                                                                                             gCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCg                                                                       >  1:224412/1‑248 (MQ=255)                                                                                                                                                                 gATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCaaa                                                                >  1:28706/1‑251 (MQ=255)                                                                                                                                                                      ggCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGaatt                                                           >  1:360832/1‑251 (MQ=255)                                                                                                                                                                            gCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCgtgt                                                                           >  1:863444/1‑229 (MQ=255)                                                                                                                                                                             ccTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGaattaatt                                                       <  1:119144/248‑1 (MQ=255)                                                                                                                                                                                    ctcGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATaa                                             <  1:410702/251‑1 (MQ=255)                                                                                                                                                                                      cgcccgccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAg                                           <  1:899684/251‑1 (MQ=255)                                                                                                                                                                                      cgcccgccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTCTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAg                                           <  1:376517/251‑1 (MQ=255)                                                                                                                                                                                        cccgccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACCCCATAAAGCg                                         <  1:892089/251‑1 (MQ=255)                                                                                                                                                                                           gccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGaa                                                                                                                            >  1:767417/1‑165 (MQ=255)                                                                                                                                                                                            ccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTAc                                                                                                                                              <  1:870549/146‑1 (MQ=255)                                                                                                                                                                                            ccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                                                                                                     <  1:169768/155‑1 (MQ=255)                                                                                                                                                                                            ccTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTaa                                      >  1:156473/1‑250 (MQ=255)                                                                                                                                                                                               ccAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGtt                                                                                                       <  1:477425/182‑1 (MQ=255)                                                                                                                                                                                               ccAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAAt                                  >  1:371483/1‑251 (MQ=255)                                                                                                                                                                                                          tGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcag                        <  1:331277/250‑3 (MQ=255)                                                                                                                                                                                                          tGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcag                        <  1:531213/250‑3 (MQ=255)                                                                                                                                                                                                           gAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcagcg                      >  1:755563/1‑251 (MQ=255)                                                                                                                                                                                                            aGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAgcagcg                      >  1:403211/1‑250 (MQ=255)                                                                                                                                                                                                               tAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGa                                                              <  1:541663/207‑1 (MQ=255)                                                                                                                                                                                                                        cGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAgtt         >  1:556795/1‑251 (MQ=255)                                                                                                                                                                                                                             gATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAgtttgttt    >  1:770728/1‑251 (MQ=255)                                                                                                                                                                                                                               tCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGtttt   >  1:709317/1‑250 (MQ=255)                                                                                                                                                                                                                                ccGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTc  >  1:482220/1‑250 (MQ=255)                                                                                                                                                                                                                                  gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACAtt                                                                                                                                           >  1:617908/1‑111 (MQ=255)                                                                                                                                                                                                                                  gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                                                                                                <  1:871816/122‑1 (MQ=255)                                                                                                                                                                                                                                  gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGc                                          >  1:74681/1‑208 (MQ=255)                                                                                                                                                                                                                                  gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTc  >  1:806905/1‑248 (MQ=255)                                                                                                                                                                                                                                  gTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCAGCGCGCCAGGCGAGTTTGTTTTc  >  1:543343/1‑248 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            GTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCACGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGTACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTCCGTGTCCCGCTGCAAAGAATTAATTAACTCCATAAAGCGTAACAATGCCGTAGCGGCGCGCCCGGCGAGTTTGTTTTC  >  1/1854529‑1855001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑