Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,853,714	A→T	intergenic (+58787/‑80617)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,853,714	0	A	T	100.0%	168.6 / NA	62	intergenic (+58787/‑80617)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (39/23);  total (39/23)

AACGTGATCAATTTGCCGACATTTCTTCTATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGAGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCGGCGCCGTCATTACGTAT  >  1/1853470‑1853960                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       aaCGTGATCAATTTGCCGACATTTCTTCTATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGc                                                                                                                                                                                                                                                  <  1:120322/251‑1 (MQ=255)    gTGATCAATTTGCCGACATTTCTTCTATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCAtt                                                                                                                                                                                                                                               >  1:58787/1‑251 (MQ=255)                       ttcttcTATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATg                                                                                                                                                                                                                            <  1:33256/251‑1 (MQ=255)                       ttcttcTATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATAATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATg                                                                                                                                                                                                                            >  1:67379/1‑251 (MQ=255)                         cttctATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATgcg                                                                                                                                                                                                                          >  1:84807/1‑251 (MQ=255)                         cttctATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATg                                                                                                                                                                                                                            >  1:861028/1‑249 (MQ=255)                                          tATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTc                                                                                                                                                                                                                                            <  1:102217/216‑1 (MQ=255)                                          tATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACg                                                                                                                                                                                                         >  1:226969/1‑251 (MQ=255)                                               ggACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCAt                                                                                                                                                                                                                                                >  1:399474/1‑207 (MQ=255)                                               ggACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGtt                                                                                                                                                                                                    >  1:755906/1‑251 (MQ=255)                                                gACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCATAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAg                                                                                                                                                                                                      <  1:454505/248‑1 (MQ=255)                                                      ggCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATg                                                                                                                                                                                                                            >  1:506995/1‑220 (MQ=255)                                                           gAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTc                                                                                                                                                                                         >  1:468033/1‑250 (MQ=255)                                                                 aTTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTaa                                                                                                                                                                                  >  1:200340/1‑251 (MQ=255)                                                                        cTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAg                                                                                                                                                                                >  1:247216/1‑246 (MQ=255)                                                                               tCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGa                                                                                                                                                                     >  1:634696/1‑250 (MQ=255)                                                                                               cGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTg                                                                                                                                                    >  1:470155/1‑251 (MQ=255)                                                                                                         gaCTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACgaaga                                                                                                                                                               <  1:411144/230‑1 (MQ=255)                                                                                                           cTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATg                                                                                                                                                                                                                            <  1:328421/167‑1 (MQ=255)                                                                                                                   cccGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCAcaaaggcaa                                                                                                                                 <  1:716668/250‑1 (MQ=255)                                                                                                                         tGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAg                                                                                                                          >  1:658111/1‑251 (MQ=255)                                                                                                                           gCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGtt                                                                                                                        >  1:665483/1‑251 (MQ=255)                                                                                                                                aTTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGc                                                                                                                            >  1:660346/1‑242 (MQ=255)                                                                                                                                aTTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGaaa                                                                                                                   >  1:126191/1‑251 (MQ=255)                                                                                                                                aTTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGaaa                                                                                                                   >  1:563508/1‑251 (MQ=255)                                                                                                                                 ttAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAg                                                                                                                  <  1:362334/251‑1 (MQ=255)                                                                                                                                        ccTATTTGGCGCAAAACCCAAGCGACTCTATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGACACCGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACgaaga                                                                                                                                                               >  1:31052/1‑199 (MQ=255)                                                                                                                                           aTTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGa                                                                                                        <  1:115632/251‑1 (MQ=255)                                                                                                                                            tttGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGAt                                                                                                       <  1:494394/251‑1 (MQ=255)                                                                                                                                                gcgcAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGc                                                                                                                                                                                                                                      <  1:287121/120‑1 (MQ=255)                                                                                                                                                 cgcAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTc                                                                                                  <  1:438286/251‑1 (MQ=255)                                                                                                                                                                aCTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACGACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTc                                                                                                                                                                                         <  1:195959/149‑1 (MQ=255)                                                                                                                                                                   atatTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCAcc                                                                                                                                                        >  1:70314/1‑179 (MQ=255)                                                                                                                                                                   atatTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGa                                                                                >  1:631576/1‑251 (MQ=255)                                                                                                                                                                            ttattGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGc                                                                                                                                                           >  1:543595/1‑167 (MQ=255)                                                                                                                                                                            ttattGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCAcaaaggc                                                                                                                                   <  1:452343/191‑1 (MQ=255)                                                                                                                                                                            ttattGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAg                                                                                                                  >  1:376494/1‑208 (MQ=255)                                                                                                                                                                            ttattGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAAtt                                                                        >  1:557466/1‑250 (MQ=255)                                                                                                                                                                                      gATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATAc                                                                                                                                                                                           >  1:26350/1‑125 (MQ=255)                                                                                                                                                                                        ttCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCg                                                           >  1:337933/1‑251 (MQ=255)                                                                                                                                                                                          ccTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACgaaagaa                                                                                                                                                                                                             <  1:786005/103‑1 (MQ=255)                                                                                                                                                                                          ccTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTc                                                                       <  1:613782/237‑1 (MQ=255)                                                                                                                                                                                          ccTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCggg                                                         >  1:900250/1‑251 (MQ=255)                                                                                                                                                                                           cTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTc                                                                                                                                                                                         >  1:17815/1‑122 (MQ=255)                                                                                                                                                                                                    gCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAAt                                                                  >  1:114/1‑232 (MQ=255)                                                                                                                                                                                                    gCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTg                                               >  1:409151/1‑251 (MQ=255)                                                                                                                                                                                                      ccACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCAcaaaggcaa                                                                                                                                 >  1:711741/1‑167 (MQ=255)                                                                                                                                                                                                         caacaTATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCaaa                                                                                                                                                                                              <  1:806332/103‑1 (MQ=255)                                                                                                                                                                                                         caacaTATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCt                                                                                                   >  1:504562/1‑194 (MQ=255)                                                                                                                                                                                                          aacaTATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCAcaaagg                                                                                                                                    >  1:264274/1‑160 (MQ=255)                                                                                                                                                                                                                    gCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAAc                                >  1:572336/1‑250 (MQ=255)                                                                                                                                                                                                                                gcagGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAAt                                                                  <  1:724377/204‑1 (MQ=255)                                                                                                                                                                                                                                 cagGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGAt                     <  1:281083/248‑1 (MQ=255)                                                                                                                                                                                                                                  agGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCaaa                                                                                                                                                                                              >  1:657903/1‑78 (MQ=255)                                                                                                                                                                                                                                  agGTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCgg                  >  1:617872/1‑250 (MQ=255)                                                                                                                                                                                                                                   ggTGGAACAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATc                    >  1:135635/1‑247 (MQ=255)                                                                                                                                                                                                                                          cAGCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCGGCGCCGTCAt         >  1:869814/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCaaa                                                                                                                                                                                              <  1:134775/68‑1 (MQ=255)                                                                                                                                                                                                                                            gCTCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGgatg                                           <  1:636160/215‑1 (MQ=255)                                                                                                                                                                                                                                              tCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCGGCGCCGTCATTACg     <  1:800666/251‑1 (MQ=255)                                                                                                                                                                                                                                              tCAAAGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCGGCGCCGTCATTACg     <  1:875722/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaGGTGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCGGCGCCGTCATTACGtat  >  1:344493/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       AACGTGATCAATTTGCCGACATTTCTTCTATTGTTCGCGCTTATCAGGACAAAGGCTGGAATAAATTAAAGCTGTATGTCTTTGATGTGCCTGACGCTGAAGGAGACTTATTTGCCCGCTTGGCACAATTAAAAACCTATTTGGCGCAAAACCCAAGCGACTATATTGAAATTATTGAACAGATTCCTATTGAGAGCCCACAACATATTCAGCAATTTTTGCAGCAGGTGGAACAGCTCAAAGGAGAAGGCATTGTCATTCGCAATCCTAATGCGCCCTACGAAAGAAAACGCAGTTCGCAAATACTCAAATTAAAGACCGCACTTGACGAAGAATGCACCGTTGTCGCCCACCACAAAGGCAAAGGGCAGTTTGAAAGTGTGATGGGATCGCTCACTTGCGAAAACCATCGAGGGCAATTCAAAATCGGTTCGGGTTTCAAGTTGGATGATCGCATTAACCCGCCCCCGATCGGCGCCGTCATTACGTAT  >  1/1853470‑1853960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑