Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,850,334	A→G	intergenic (+55407/‑83997)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,850,334	0	A	G	100.0%	164.2 / NA	61	intergenic (+55407/‑83997)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/31);  total (30/31)

CTACGATCGCAACAAAAAGTTAATTTTAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACATCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAAAATTAAATTTAATCGAAAAGAATTTGTCTCATACTTACTGAATATCGGCATTATTTTTGCCTTTTTATTGGGTTTAATTTATTTATCG  >  1/1850087‑1850567                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          cTACGATCGCAACAAAAAGTTAATTTTAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGACTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCg                                                                                                                                                                                                                                        >  1:341206/1‑251 (MQ=255)   aCGATCGCAACAAAAAGTTAATTTTAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGtt                                                                                                                                                                                                                                      >  1:63795/1‑251 (MQ=255)                  aGTTAATTTTAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGa                                                                                                                                                                                                                        >  1:354152/1‑250 (MQ=255)                       aTTTTAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCtgtg                                                                                                                                                                                                                  >  1:586913/1‑251 (MQ=255)                          ttAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCTCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCAc                                                                                                                                                                                                               <  1:803120/251‑1 (MQ=255)                          ttAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCAc                                                                                                                                                                                                               <  1:99572/251‑1 (MQ=255)                          ttAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCAc                                                                                                                                                                                                               <  1:828759/251‑1 (MQ=255)                          ttAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCAc                                                                                                                                                                                                               <  1:294602/251‑1 (MQ=255)                              gCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGt                                                                                                                                                                                                            <  1:74542/250‑1 (MQ=255)                               cACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCGGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTAc                                                                                                                                                                                                          <  1:539946/251‑1 (MQ=255)                               cACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTAc                                                                                                                                                                                                          <  1:601138/251‑1 (MQ=255)                                   tCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGc                                                                                                                                                                                                       <  1:511455/250‑1 (MQ=255)                                        atatTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGc                                                                                                                                                                                                   >  1:56133/1‑249 (MQ=255)                                             ccTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCtattat                                                                                                                                                                                            <  1:237315/251‑1 (MQ=255)                                              cTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGc                                                                                                                                                                                                       >  1:609699/1‑239 (MQ=255)                                                 cTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTAttatttat                                                                                                                                                                                        <  1:649802/251‑1 (MQ=255)                                                   aTTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTc                                                                                                                                                                                      <  1:453203/251‑1 (MQ=255)                                                          cGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTgg                                                                                                                                                                               >  1:171358/1‑251 (MQ=255)                                                            ccATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGAcc                                                                                                                                                                                                                      >  1:816153/1‑210 (MQ=255)                                                                tttATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAA‑CATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACaa                                                                                                                                                                        <  1:574095/251‑1 (MQ=255)                                                                 ttATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACaa                                                                                                                                                                        <  1:717390/251‑1 (MQ=255)                                                                       gcgcCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGCTAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACCATCCggg                                                                                                                                                                  >  1:255014/1‑250 (MQ=255)                                                                               gttgtATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGt                                                                                                                                                                                                            >  1:2670/1‑201 (MQ=255)                                                                                  gtATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCt                                                                                                                                                                            >  1:834028/1‑230 (MQ=255)                                                                                          gCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATcc                                                                                                                                                                     <  1:61211/229‑1 (MQ=255)                                                                                          gCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCaaa                                                                                                                                               >  1:543404/1‑251 (MQ=255)                                                                                                  ttGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCAc                                                                                                                                                                                                               >  1:411271/1‑179 (MQ=255)                                                                                                  ttGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCAc                                                                                                                                       <  1:610418/251‑1 (MQ=255)                                                                                                    gCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTc                                                                                                                                                                                                     <  1:443960/187‑1 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGAcc                                                                                                                                                                                                                      >  1:88702/1‑165 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTGCCGCTCGCCTattatt                                                                                                                                                                                           <  1:191556/192‑1 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTAc                                                                                                                                                                                                          <  1:532714/177‑1 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTAc                                                                                                                                                                                                          >  1:351479/1‑177 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTAc                                                                                                                                                                          >  1:613214/1‑209 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTc                                                                                                                                            <  1:839332/239‑1 (MQ=255)                                                                                                         cccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGCTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCctca                                                                                                                                >  1:746153/1‑251 (MQ=255)                                                                                                          ccTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCctcac                                                                                                                               <  1:148134/251‑1 (MQ=255)                                                                                                                   gggCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCctcactc                                                                                                                             >  1:691018/1‑244 (MQ=255)                                                                                                                        aTCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCtattat                                                                                                                                                                                            <  1:355063/176‑1 (MQ=255)                                                                                                                        aTCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATc                                                                                                                                                                      <  1:54322/198‑1 (MQ=255)                                                                                                                             gtgCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCAcc                                                                                                                                      <  1:675621/225‑1 (MQ=255)                                                                                                                                  gCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCctcactc                                                                                                                             <  1:185193/229‑1 (MQ=255)                                                                                                                                     atatTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGc                                                                                                                                                                                                                 >  1:64708/1‑142 (MQ=255)                                                                                                                                      tatTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTttat                                                                                                   <  1:324701/251‑1 (MQ=255)                                                                                                                                      tatTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTttat                                                                                                   <  1:503649/251‑1 (MQ=255)                                                                                                                                      tatTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTttat                                                                                                   <  1:184515/251‑1 (MQ=255)                                                                                                                                          ccGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTaa                                                                                                >  1:341006/1‑250 (MQ=255)                                                                                                                                                  cTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGAcc                                                                                                                                                                                                                      <  1:676107/124‑1 (MQ=255)                                                                                                                                                  cTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCctcactc                                                                                                                             >  1:209775/1‑213 (MQ=255)                                                                                                                                                    ggTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAaaatta                                                                                     >  1:851248/1‑251 (MQ=255)                                                                                                                                                                          tCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTc                                                                                                             >  1:300880/1‑205 (MQ=255)                                                                                                                                                                          tCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAAAATTAAATTTAATCGAAAAGAATTTGt                                                               >  1:304035/1‑251 (MQ=255)                                                                                                                                                                          tCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGAACCGCTCGCCTATTATTTATTcac                                                                                                                                                                                    >  1:162901/1‑134 (MQ=255)                                                                                                                                                                           ccTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCAc                                                                                                                                                                                                               >  1:278821/1‑106 (MQ=255)                                                                                                                                                                                 tCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAAAATTAAATTTAATCGAAAAGAATTTGTCTCAtac                                                        <  1:35910/251‑1 (MQ=255)                                                                                                                                                                                    gcgcCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTAc                                                                                                                 >  1:420566/1‑191 (MQ=255)                                                                                                                                                                                               ggTTCCTACTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGt                                                                                                                                                                                                            <  1:186523/89‑1 (MQ=255)                                                                                                                                                                                                         ggATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAAAATTAAATTTAATCGAAAAGAATTTGTCTCATACTTACTGAATATCgg                                          >  1:618158/1‑241 (MQ=255)                                                                                                                                                                                                                                       ccATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGt                                                                                                                                                                                                            <  1:393241/49‑1 (MQ=255)                                                                                                                                                                                                                                       ccATCAAACGATACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAAAATTAAATTTAATCGAAAAGAATTTGTCTCATACTTACTGAATATCGGCATTATTTTTGCCTTTTTATTGGGTTTAATTTATTTATCg  >  1:809327/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aTACAACGTCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTAc                                                                                                                 >  1:857338/1‑130 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          CTACGATCGCAACAAAAAGTTAATTTTAGGCACGTCAAAATATTCCTTCTATTTAATCGCCATTTTATCGGCGCCGGTGTTGTATTTCAGCCATACATTGCTCACCCTTTGGCTGGGCGATCATGTGCCGCAATATTCCGATAAACTGGTGCAAGCCACCATTGTTTGTTCCTTAATCAGCGCCATGGCGGGTTCCTTCTGGATGACGGCGCTTGCCATCGGTTCGCACACCATCAAACGATACAACATCGTTTTGGCAACCATTGACCTGTGCACCGTACCGCTCGCCTATTATTTATTCACACTGGGCTACAATCCGGTGTTTGCGTTCATCGGCAAATTCTGCACCGCCTCACTCATGCAAATTTACCGTCTGTATTTTATTAACAACAAAATTAAATTTAATCGAAAAGAATTTGTCTCATACTTACTGAATATCGGCATTATTTTTGCCTTTTTATTGGGTTTAATTTATTTATCG  >  1/1850087‑1850567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑