Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	372,028	A→G	intergenic (‑27105/+39848)	unknown ← / ← unknown	tRNA‑Ser‑GCT/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	372,028	0	A	G	100.0%	83.7 / NA	32	intergenic (‑27105/+39848)	unknown/unknown	tRNA‑Ser‑GCT/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/8);  total (24/8)

GCGTCAACCCACAATTTGACAGGAGTAAAGGTCAATGACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACCCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGATAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAATTTTAGCTACCACGCCGGCACCCACTGTACGGCCACCTTCACGGATAGCGAAACGTAAACCTTG  >  1/371789‑372264                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             gCGTCAACCCACAATTTGACAGGAGTAAAGGTCAATGACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCtt                                                                                                                                                                                                                                   >  1:26591/1‑251 (MQ=255)       aCCCACAATTTGACAGGAGTAAAGGTCAATGACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGcgatgaat                                                                                                                                                                                                                              >  1:597137/1‑243 (MQ=255)                       gAGTAAAGGTCAATGACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATg                                                                                                                                                                                                                              <  1:376165/234‑1 (MQ=255)                       gAGTAAAGGTCAATGACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTAttt                                                                                                                                                                                                                       <  1:394065/241‑1 (MQ=255)                                     gACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGGCCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATg                                                                                                                                                                                                                              <  1:302132/220‑1 (MQ=255)                                      aCTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGt                                                                                                                                                                                               <  1:224533/250‑1 (MQ=255)                                            gCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGtat                                                                                                                                                                                        >  1:29716/1‑251 (MQ=255)                                                          gCATAGCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAg                                                                                                                                                                          >  1:499380/1‑251 (MQ=255)                                                               gCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAAt                                                                                                                                                                                                                               >  1:411958/1‑193 (MQ=255)                                                               gCTATGCATAAATAATGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGt                                                                                                                                                                     >  1:802522/1‑251 (MQ=255)                                                                       taaataaTGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCAt                                                                                                                                                              >  1:813837/1‑250 (MQ=255)                                                                           taatGTCACCTGCCTACTCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCgtg                                                                                                                                                                                           >  1:112643/1‑217 (MQ=255)                                                                                                   gTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGtatttatt                                                                                                                                                    >  1:571439/1‑232 (MQ=255)                                                                                                                                      aTGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATc                                                                                                                                                                                                                                        <  1:279924/113‑1 (MQ=255)                                                                                                                                                 tATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGt                                                                                     >  1:385342/1‑249 (MQ=255)                                                                                                                                                      ggCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCCTAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCAATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCa                                                                              >  1:424828/1‑251 (MQ=255)                                                                                                                                                         ttGAGGTGTTGCTGAGAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAatt                                                                           <  1:458591/251‑1 (MQ=255)                                                                                                                                                             ggTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAAt                                                                                                                                                                                                                    <  1:261102/110‑1 (MQ=255)                                                                                                                                                                   gCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAAt                                                                 >  1:85975/1‑251 (MQ=255)                                                                                                                                                                                   ttACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATg                                                                                                                                                                                    >  1:97304/1‑120 (MQ=255)                                                                                                                                                                                     aCAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAAt                                                                                                                                                                                                                    >  1:378160/1‑86 (MQ=255)                                                                                                                                                                                      cAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAAt                                                                                                                                                                                                                    >  1:707771/1‑85 (MQ=255)                                                                                                                                                                                                 attattCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCa                                                                              >  1:712266/1‑208 (MQ=255)                                                                                                                                                                                                    attCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGtat                                                                                                                                                         >  1:469120/1‑130 (MQ=255)                                                                                                                                                                                                           tCTGAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATg                                                                                                                                                                                                                              >  1:94997/1‑54 (MQ=255)                                                                                                                                                                                                              gAACGATATACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCaa                                                                             >  1:211356/1‑196 (MQ=255)                                                                                                                                                                                                                   atatACAGATTCAGACATTGAAAAATACGGTAAATc                                                                                                                                                                                                                                        <  1:388573/36‑1 (MQ=255)                                                                                                                                                                                                                   atatACAGATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAATTTTAGCTACCACGCCGGCACCCACTGTACGGCCACCTTCACGGATAg                  >  1:658488/1‑250 (MQ=255)                                                                                                                                                                                                                          gATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGAt                                                                                                                 >  1:889600/1‑148 (MQ=255)                                                                                                                                                                                                                          gATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAATTTTAGCTACCACGCCGGCACCCACTGTACGGc                                 >  1:82516/1‑228 (MQ=255)                                                                                                                                                                                                                          gATTCAGACATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAATTTTAGCTACCACGCCGGCACCCACTGTACGGCCACCTTCACGGATAGCGAAACGt          >  1:64600/1‑251 (MQ=255)                                                                                                                                                                                                                                  cATTGAAAAATACGGTAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAATTTTAGCTACCACGCCGGCACCCACTGTACGGCCACCTTCACGGATAGCGAAACGTAAACCTTg  >  1:76015/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             GCGTCAACCCACAATTTGACAGGAGTAAAGGTCAATGACTATGGCTAAATACAGTCAGCATAGCTATGCATAAATAATGTCACCTGCCTACCCCCCCGGTTTTTGCCTGCACATTAACTTGTTAATTTAACTAATGATTGGTTTTATCAGGCTTGAGGTGTTGCTGAAAGTTAGACCGTTACAATATGGACTATTATTCACGTCTGAACGATATACAGATTCAGACATTGAAAAATACGATAAATCGGCTTAAATGATTATTTAATAGAATTAAAATAAAAATTAGTCGTGTATAATGAATTAAAAAGGAAGTACAGCATAGTATTTATTAAAGATTTCATAAACAAAAAGGCGTACCTCTCGATACGCCTTTTCAGTTTTACTTTGGTTTGTCTTATCAATTATTTGATAATTTTAGCTACCACGCCGGCACCCACTGTACGGCCACCTTCACGGATAGCGAAACGTAAACCTTG  >  1/371789‑372264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑