Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,977	G→A	intergenic (+37050/‑102354)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,977	0	G	A	100.0%	154.3 / NA	58	intergenic (+37050/‑102354)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/36);  total (22/36)

CATTTGGCAAAGGTTGATAGCAAGGCTTTGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAGCCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGTTTACCAT  >  1/1831738‑1832221                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     cATTTGGCAAAGGTTGATAGCAAGGCTTTGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAacac                                                                                                                                                                                                                                           <  1:242233/251‑1 (MQ=255)     tGGCAAAGGTTGATAGCAAGGCTTTGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGATTCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCAtt                                                                                                                                                                                                                                       >  1:188656/1‑251 (MQ=255)                         gCTTTGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAAC‑TTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATc                                                                                                                                                                                                                   <  1:213564/250‑1 (MQ=255)                          cTTTGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATc                                                                                                                                                                                                                   <  1:851993/250‑1 (MQ=255)                            ttGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTc                                                                                                                                                                                                                <  1:289745/251‑1 (MQ=255)                                  aaCAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTaa                                                                                                                                                                                                          >  1:688441/1‑251 (MQ=255)                                            gTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGtatta                                                                                                                                                                                                >  1:189298/1‑251 (MQ=255)                                             tGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGtatta                                                                                                                                                                                                <  1:475349/250‑1 (MQ=255)                                                 ttttttACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAgg                                                                                                                                                                                           <  1:387971/251‑1 (MQ=255)                                                               tttGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACatat                                                                                                                                                                                                                    <  1:618926/212‑1 (MQ=255)                                                                ttGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGttc                                                                                                                                                                            <  1:420948/251‑1 (MQ=255)                                                                          aaaGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAacac                                                                                                                                                                   <  1:341309/250‑1 (MQ=255)                                                                                    cttcttTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGAt                                                                                                                                                        >  1:338070/1‑251 (MQ=255)                                                                                              tCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATAc                                                                                                                                                      <  1:204999/243‑1 (MQ=255)                                                                                                tGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAag                                                                                                                                            <  1:662812/251‑1 (MQ=255)                                                                                                tGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAag                                                                                                                                            <  1:51181/251‑1 (MQ=255)                                                                                                        tttttCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGAc                                                                                                                                    <  1:179722/251‑1 (MQ=255)                                                                                                          tttCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAag                                                                                                                                            >  1:250308/1‑241 (MQ=255)                                                                                                                            aTCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGc                                                                                                                 >  1:564112/1‑250 (MQ=255)                                                                                                                                 cccTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAag                                                                                                           >  1:563807/1‑251 (MQ=255)                                                                                                                                 cccTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAag                                                                                                           <  1:868835/251‑1 (MQ=255)                                                                                                                                       ttCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGa                                                                                                      <  1:553676/250‑1 (MQ=255)                                                                                                                                           tGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATc                                                                                                 <  1:854855/251‑1 (MQ=255)                                                                                                                                             cTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTg                                                                                               <  1:357513/251‑1 (MQ=255)                                                                                                                                                     cGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGAt                                                                                       <  1:721712/251‑1 (MQ=255)                                                                                                                                                      gCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTaa                                                                                                                    <  1:831032/221‑1 (MQ=255)                                                                                                                                                                        ttGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCg                                                                    >  1:70835/1‑251 (MQ=255)                                                                                                                                                                                    gCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATc                                                                                                                                                                                                                   <  1:541582/96‑1 (MQ=255)                                                                                                                                                                                     cTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATa                                                       >  1:267170/1‑251 (MQ=255)                                                                                                                                                                                      ttAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAg                                                      <  1:666062/251‑1 (MQ=255)                                                                                                                                                                                      ttAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAg                                                      <  1:852739/251‑1 (MQ=255)                                                                                                                                                                                         aGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAgcgt                                                   >  1:842988/1‑251 (MQ=255)                                                                                                                                                                                          ggTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAg                                                      >  1:358454/1‑247 (MQ=255)                                                                                                                                                                                          ggTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAg                                                      <  1:305423/247‑1 (MQ=255)                                                                                                                                                                                           gTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCGACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGa                                                                                                      >  1:414337/1‑198 (MQ=255)                                                                                                                                                                                           gTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATAc                                                                                                                                                      <  1:525999/150‑1 (MQ=255)                                                                                                                                                                                           gTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAaga                                                                                                          <  1:118888/194‑1 (MQ=255)                                                                                                                                                                                           gTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAg                                                      >  1:217967/1‑246 (MQ=255)                                                                                                                                                                                           gTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGCTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAg                                                      <  1:441476/246‑1 (MQ=255)                                                                                                                                                                                                gTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGc                                                                                                                            <  1:184814/171‑1 (MQ=255)                                                                                                                                                                                                         aTGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTg                                                                                               <  1:410862/191‑1 (MQ=255)                                                                                                                                                                                                           gAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAgg                                  >  1:357051/1‑250 (MQ=255)                                                                                                                                                                                                            aaaTACCGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCg                                >  1:117615/1‑251 (MQ=255)                                                                                                                                                                                                                 ccGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGttcttc                                                                                                                                                                         <  1:136993/109‑1 (MQ=255)                                                                                                                                                                                                                 ccGTTGCTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGaa                           <  1:279250/251‑1 (MQ=255)                                                                                                                                                                                                                       cTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCg                     <  1:651107/251‑1 (MQ=255)                                                                                                                                                                                                                       cTTCCGCACAATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCg                     >  1:19960/1‑251 (MQ=255)                                                                                                                                                                                                                               caATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTaa                                                           <  1:689154/205‑1 (MQ=255)                                                                                                                                                                                                                               caATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGaa                           >  1:828219/1‑237 (MQ=255)                                                                                                                                                                                                                               caATAGGCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGCTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAAt                                                        >  1:736300/1‑208 (MQ=255)                                                                                                                                                                                                                                     gCAACTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGt         <  1:122263/249‑1 (MQ=255)                                                                                                                                                                                                                                       aaCTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGTTTAc     <  1:604813/251‑1 (MQ=255)                                                                                                                                                                                                                                        aCTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGTTTAc     >  1:541728/1‑250 (MQ=255)                                                                                                                                                                                                                                         cTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGta                                                                                                                                                                                                   <  1:65549/59‑1 (MQ=255)                                                                                                                                                                                                                                         cTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGttc                                                                                                                                                                            >  1:866138/1‑82 (MQ=255)                                                                                                                                                                                                                                         cTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTa                                                            >  1:922/1‑194 (MQ=255)                                                                                                                                                                                                                                         cTTTATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGTTTACCa   >  1:67781/1‑251 (MQ=255)                                                                                                                                                                                                                                          tttATAACCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGTTTACCAt  <  1:31240/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CATTTGGCAAAGGTTGATAGCAAGGCTTTGGGTAACAAGTGCGGTGCATTTTTTACAAGAAATTTGAGTTCAGAAAGCACAAACTTCTTTCCTTCTGAAGCCATTTTTCCAAAGGTTTGCTGAATCCACCCTTGTTCTTGCTGAAACACGCCTGTGTCAAAATAGCGTTGTAATTCCTGGCTTAAGGTATAGTTGTGGCTATGAAATACCGTTGCTTCCGCACAATAGGCAACTTTATAGCCTGCTAACACCATTTTGGCGGTTAAGTACATATCTTCCGCTAAAATTGTATTATCAGGAAAGCCCCCAAGTTCTTCAAACACCGAGCGACGATACGCCGCAAAAGAGTTCGACATAAAAGCCGTTTTAATGCCTAAAGAGGGAATATCTGCTTTTGATTTTAATTTCGATTGCGCCGGGTAATTAAAATAGCGTGCGTGAGCGGCAACAGGCGTGGAATTAGCGTGTGGCAACTGTTTACCAT  >  1/1831738‑1832221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑