Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,823,571	G→A	intergenic (+28644/‑110760)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,823,571	0	G	A	100.0%	177.8 / NA	66	intergenic (+28644/‑110760)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/34);  total (32/34)

AAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGACACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCGCCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCT  >  1/1823327‑1823814                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aaGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGtat                                                                                                                                                                                                                                               <  1:515682/251‑1 (MQ=255) aaGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGtat                                                                                                                                                                                                                                               <  1:211432/251‑1 (MQ=255)     cATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaat                                                                                                                                                                                                                                           <  1:393209/251‑1 (MQ=255)     cATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaat                                                                                                                                                                                                                                           <  1:883919/251‑1 (MQ=255)     cATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                                                                                                                                                                                                                                            <  1:351456/250‑1 (MQ=255)      aTTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaata                                                                                                                                                                                                                                          <  1:752291/251‑1 (MQ=255)                         gATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGtat                                                                                                                                                                                                                                               >  1:58399/1‑227 (MQ=255)                         gATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATccg                                                                                                                                                                                                                       >  1:619144/1‑251 (MQ=255)                              gAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATTATTGAAATTGGACAATccgccgc                                                                                                                                                                                                                   >  1:832750/1‑250 (MQ=255)                                     gcacCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTAc                                                                                                                                                                                                            <  1:611273/249‑1 (MQ=255)                                       acCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCACCGAGTACGAt                                                                                                                                                                                                         <  1:556908/251‑1 (MQ=255)                                        ccTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaata                                                                                                                                                                                                                                          >  1:538514/1‑217 (MQ=255)                                        ccTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGAt                                                                                                                                                                                                         >  1:89629/1‑250 (MQ=255)                                        ccTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGAt                                                                                                                                                                                                         >  1:589413/1‑250 (MQ=255)                                             gATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATg                                                                                                                                                                                                   >  1:618977/1‑251 (MQ=255)                                              aTAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGc                                                                                                                                                                                                  <  1:641674/251‑1 (MQ=255)                                                          aaCCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATg                                                                                                                                                                                                   <  1:717340/238‑1 (MQ=255)                                                          aaCCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCg                                                                                                                                                                                      >  1:635954/1‑251 (MQ=255)                                                           aCCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGCTTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                                                                                                                                                                                                                                            >  1:420182/1‑196 (MQ=255)                                                            ccATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                                                                                                                                                                                                                                            <  1:762731/195‑1 (MQ=255)                                                             cATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCGGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaat                                                                                                                                                                                                                                           <  1:840505/195‑1 (MQ=255)                                                                  tttCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGat                                                                                                                                                                              <  1:438671/251‑1 (MQ=255)                                                                         cAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCg                                                                                                                                                                       <  1:9141/251‑1 (MQ=255)                                                                           aaaaTAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGAt                                                                                                                                                                     >  1:594663/1‑251 (MQ=255)                                                                            aaaTAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATg                                                                                                                                                                    >  1:364175/1‑251 (MQ=255)                                                                              aTAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGa                                                                                                                                                                  >  1:335007/1‑251 (MQ=255)                                                                                       gAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTTGGGAAGGCACCGTTTAAATAATCGAAATTGGACAATCCGCCGCCGCGGACGATCa                                                                                                                                                                                                       >  1:134657/1‑205 (MQ=255)                                                                                       gAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                                                                                                                                                                                                                                            >  1:876423/1‑168 (MQ=255)                                                                                             ggATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGAt                                                                                                                                                                                                         <  1:713656/197‑1 (MQ=255)                                                                                                    gCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATg                                                                                                                                                                                >  1:41519/1‑215 (MQ=255)                                                                                                    gCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGc                                                                                                                                                                         <  1:126699/222‑1 (MQ=255)                                                                                                     ccTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACaaa                                                                                                                                             <  1:525643/249‑1 (MQ=255)                                                                                                        tttttATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                                                                                                                                                                                                                                            <  1:605647/151‑1 (MQ=255)                                                                                                              ttGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACaa                                                                                                                                  <  1:867922/251‑1 (MQ=255)                                                                                                                 cGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTAc                                                                                                                                                                                                            >  1:158798/1‑174 (MQ=255)                                                                                                                     aaaCATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                                                                                                                                                                                                                                            <  1:856078/138‑1 (MQ=255)                                                                                                                       aCATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGat                                                                                                                                                                              <  1:860557/198‑1 (MQ=255)                                                                                                                        cATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCg                                                                                                                         >  1:45370/1‑250 (MQ=255)                                                                                                                                ggATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTAt                                                                                                                >  1:460519/1‑251 (MQ=255)                                                                                                                                  aTAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCg                                                                                                              <  1:834085/251‑1 (MQ=255)                                                                                                                                              ccgcGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGaa                                                                                                   <  1:658108/250‑1 (MQ=255)                                                                                                                                                   gcgcCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTg                                                                                             >  1:851696/1‑251 (MQ=255)                                                                                                                                                           cacgcCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAAttt                                                                                     <  1:614966/251‑1 (MQ=255)                                                                                                                                                                         cGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAg                                                                       >  1:95645/1‑251 (MQ=255)                                                                                                                                                                                       tttttAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCGCTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGcc                                                               <  1:786499/245‑1 (MQ=255)                                                                                                                                                                                               cACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGGAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAACGCGGGTCAAAGGCGGGAATGATATTGCCGATGGAAATCGCGGCAAGCCCCACAAAAACGCCGACAAAATCCACCGCCCCTTTATCGCTCGCGTAGAAAAGTTGAATAATTTCTTTGGCCGATTAGGATTCGCCTTTTAAATCGCGGt                                                 >  1:742911/1‑251 (MQ=255)                                                                                                                                                                                                    gCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATaaa                                            >  1:769952/1‑250 (MQ=255)                                                                                                                                                                                                            gcgcTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACAt                                          >  1:807714/1‑245 (MQ=255)                                                                                                                                                                                                               cTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAAc                                  <  1:334905/250‑1 (MQ=255)                                                                                                                                                                                                                    cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGa                                                                     >  1:690646/1‑210 (MQ=255)                                                                                                                                                                                                                    cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAAc                                            >  1:375141/1‑235 (MQ=255)                                                                                                                                                                                                                    cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGa                             >  1:473112/1‑250 (MQ=255)                                                                                                                                                                                                                    cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGa                             >  1:349153/1‑250 (MQ=255)                                                                                                                                                                                                                               gcggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTa                                                          >  1:505283/1‑210 (MQ=255)                                                                                                                                                                                                                               gcggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAg                 >  1:861776/1‑251 (MQ=255)                                                                                                                                                                                                                                cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                <  1:616008/251‑1 (MQ=255)                                                                                                                                                                                                                                cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                <  1:62504/251‑1 (MQ=255)                                                                                                                                                                                                                                cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                <  1:709260/251‑1 (MQ=255)                                                                                                                                                                                                                                cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAg                 <  1:606210/250‑1 (MQ=255)                                                                                                                                                                                                                                  gTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGgtg              >  1:874367/1‑251 (MQ=255)                                                                                                                                                                                                                                        cTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCaaa        >  1:812659/1‑251 (MQ=255)                                                                                                                                                                                                                                         tttCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAAc       <  1:434579/251‑1 (MQ=255)                                                                                                                                                                                                                                         tttCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAAc       <  1:153327/251‑1 (MQ=255)                                                                                                                                                                                                                                              gTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                <  1:197444/237‑1 (MQ=255)                                                                                                                                                                                                                                              gTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAgg    >  1:206167/1‑249 (MQ=255)                                                                                                                                                                                                                                               tAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCt  <  1:584278/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGACACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCGCCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCT  >  1/1823327‑1823814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑